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Composite biocontrol agent as well as preparation method and application thereof

A biocontrol fungicide and fungicide technology are applied in the field of composite biocontrol fungicide for preventing and controlling soil-borne diseases of crops and the field of preparation thereof, which can solve the problems of unsatisfactory control effect, crisis of human daily life, loss of resistance and the like

Active Publication Date: 2014-07-30
浙江延杭智能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the use of plants with disease-resistant strains can achieve cost-effective control effects, but it is not easy to obtain an ideal disease-resistant strain, and resistant varieties often appear resistant to disease after a few years of promotion. on the other hand, chemical control plays a very important role in the control of soil-borne diseases of crops due to its advantages of high control efficiency, fast speed, and wide antibacterial spectrum. Limitations of two factors: first, there are few types of high-efficiency and low-toxic chemical fungicides, and most of them are still highly toxic, so it is not a long-term solution to kill bacteria with poison; second, the impact of environmental factors on the control effect is not small , when the environmental conditions are suitable for the invasion and spread of pathogenic bacteria, the control effect is often unsatisfactory; the third is that chemical agents are likely to cause environmental pollution, endangering human daily life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 composite bacterial agent

[0021] (1) Preparation of liquid seed medium: Weigh 10g of peptone, 5g of beef extract, and 5g of NaCl, add purified water, stir and mix well, make up water to 1000mL, adjust pH=7.0, and distribute into ten 150mL Erlenmeyer flasks, 121 Sterilize at ℃ for 30 minutes.

[0022] (2) Preparation of liquid fermentation medium: Weigh 1 kg of peptone, 0.5 kg of beef extract, and 0.5 kg of NaCl, add purified water, stir and mix, make up water to 100L, adjust pH to 7.0, and sterilize at 121°C for 30 minutes.

[0023] (3) Insert Bacillus cereus into the liquid medium in the above-mentioned Erlenmeyer flask, shake and culture at 30°C and 180r / min for 30 hours to form a seed liquid; inoculate the seed liquid into the fermentation medium at a volume ratio of 1:500 Fermentation production is carried out in the liquid medium in the tank. The basic working parameters of the fermenter are: temperature 30°C, stirring speed 200...

Embodiment 2

[0026] Embodiment 2 Different bacterial strains are to the inhibition test research of Botrytis cinerea

[0027] Preparation of PDA medium: Weigh 200g of peeled potatoes, cut into pieces and boil for 30min, then filter the juice with gauze, add 20.0g of glucose and 20.0g of agar, add water to 1000mL after dissolving, and divide into 10 triangles of 150mL bottle, sterilized at 121°C for 30 minutes.

[0028] Under sterile conditions, take 20 mL of PDA medium that has been sterilized and cooled to about 45°C, and add it to a petri dish to make a PDA plate. After standing for 24 hours, inoculate the mycelium of the pathogen Botrytis cinerea in the center of the plate, and culture at 22°C for 4 days. In the gray mold plate that has grown, use a 1 cm diameter hole punch to cut out colony circles at the edge of the colony and set aside.

[0029] Take respectively the fermentation liquid of Bacillus cereus and the fermentation liquid of Pseudomonas chloropinus prepared in steps (3...

Embodiment 3

[0035] Example 3 Preparation of Biocontrol Bacterial Fertilizer

[0036] Crush the wheat straw into granules, take fresh pig manure and wheat straw granules and mix evenly at a weight ratio of 1:2.5, adjust the water content to about 32%, pile up a height of 1.2 meters, and turn the pile when the pile temperature rises to 78-80°C , and add water to keep the water content of the compost at 30-33%. After turning the compost 2-3 times, turn the compost body to make the compost temperature drop to 25-32°C, according to the access of 0.1kg compost per ton. For the prepared compound bacterial agent, the water content is adjusted to 47-48%, the heap height is 0.50 meters, the heap is turned over when the heap temperature reaches 35°C, and fermented for 5 days, and the water content of the fertilizer is evaporated to 30% under the condition that the temperature does not exceed 35°C. % below get bio-control bacterial fertilizer. The biocontrol bacterial fertilizer made by the presen...

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Abstract

The invention discloses a composite biocontrol agent for controlling crop soil-borne diseases as well as a preparation method and application of composite biocontrol agent. The composite biocontrol agent is prepared from bacillus cereus and pseudomonas chlororaphis according to a colony-forming unit number ratio of 1 : (5-20), has a good inhibition effect on botrytis cinerea, and is capable of preventing and controlling vegetable botrytis cinerea diseases to increase the vegetable yield, so that a novel biocontrol product is provided for preventing and controlling the soil-borne diseases of crops and necessary conditions are provided for developing novel bio-organic fertilizers.

Description

technical field [0001] The invention belongs to the technical field of microbial control, and in particular relates to a compound biocontrol agent for preventing and controlling soil-borne diseases of crops, a preparation method and application thereof. [0002] Background technique [0003] Soil-borne diseases of crops cause huge economic losses to agricultural production every year, so the prevention and control of diseases has become the top priority of current agricultural science. At present, the prevention and control methods of soil-borne diseases of crops are mainly based on the use of disease-resistant strains of plants and chemical control. On the one hand, the use of plants with disease-resistant strains can achieve cost-effective control effects, but it is not easy to obtain an ideal disease-resistant strain, and resistant varieties often appear resistant to disease after a few years of promotion. on the other hand, chemical control plays a very important r...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P3/00C05F11/08C05F3/00C12R1/085C12R1/38
CPCY02A40/20
Inventor 段占娥
Owner 浙江延杭智能科技有限公司
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