43 results about "Succinic dehydrogenase" patented technology

The invention relates to the field of pesticide bactericides, and discloses a difluoromethyl thiazole amide-containing compound and application thereof as a bactericide. The difluoromethyl thiazole-containing amide compound has a structure as shown in a formula (I), and the difluoromethyl thiazole-containing amide compound with efficient activity on diseases such as rice sheath blight disease is designed by introducing a difluoromethyl thiazole-containing structure and a diphenyl ether fragment. The compound can be used as a brand new succinate dehydrogenase inhibitor or bactericide. # imgabs0 #

The invention discloses a method for detecting drug resistance of botrytis cinerea to succinate dehydrogenase (SDHI) bactericides. On the basis of an RPA-CRISPR / Cas12a system, a specific primer pair amplification target fragment and crRNA for activating Cas12a trans-cleavage activity are designed aiming at key mutation sites related to drug resistance in a bactericide action target SdhB gene, so that fluorescent light visible to naked eyes can be generated only when a resistant strain sequence is matched with the crRNA, and the fluorescent light is opposite to the fluorescent light generated when a sensitive strain sequence is matched with the crRNA. The method provided by the invention overcomes the dependence on laboratories and large instruments in the traditional drug resistance detection method, provides key technical support for accurate prevention and control and drug resistance management of grape gray mold, and provides direct drug use basis for scientific prevention and control in the field.

A fungicidal composition containing a fungicidally effective amount of the compound of Formula I:5-fluoro-4-imino-3-methyl-1-tosyl-3,4-dihydropyrimidin-2(1H)-one, and at least one fungicide to provide synergistic control of selected fungi, wherein the at least one fungicide is a succinate dehydrogenase-inhibitor.

The invention discloses a preparation method and application of a multifunctional layered nanosheet. The biomimetic hydrogel is prepared through a hydrothermal method, folic acid (FA) is modified on the surface of the biomimetic hydrogel, and the biomimetic hydrogel has the capacity of targeted recognition of pro-inflammatory phenotype macrophages, has the biomimetic activity of multiple enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and can effectively remove ROS; meanwhile, a succinate dehydrogenase (SDH) inhibitor dimethylmalonic acid (DMM) is loaded in an intercalation mode of the nano material, SDH activity is inhibited in a slow release mode, ROS outbreak caused by mitochondrial reverse electron transfer (RET) is blocked from the source, then a mitochondrial autophagy pathway mediated by BNIP3-LC3B is started, and dynamic and steady state recovery of mitochondria is achieved. The system finally realizes macrophage mitochondrial function reconstruction, oxidation environment improvement and transfer of healthy mitochondria to osteoblasts, so that the osteogenic differentiation capability is recovered, and the effect of treating osteoporosis is achieved.

The invention discloses application of hydrogen-rich water in preparation of a drug for treating Duchenne type muscular dystrophy, and particularly relates to the technical field of medicines.The random control experiment is adopted, homologous c57 mice serve as a normal control group, mdx mice are randomly divided into a model group and a hydrogen-rich water group, and the exercise ability is evaluated through a four-limb holding power test and a rotating bar test; detecting mouse creatine kinase by an enzyme-linked immunosorbent assay; carrying out hematoxylin-eosin, NADH-tetrazole reductase and improved Goori three-color dyeing, and carrying out cytochrome C oxidase, succinate dehydrogenase and SDH-COX combined dyeing to observe the pathological change of the skeletal muscle tissue; the invention discloses that the muscle injury condition of a Duchenne type muscular dystrophy model mouse is improved by the hydrogen-rich water through a mitochondrial protection mechanism, and the hydrogen-rich water has a relatively high application prospect and a relatively high economic value.

The invention discloses a (-)-menthol group-containing nicotinamide derivative with antifungal activity as well as a preparation method and application thereof, and relates to the technical field of synthesis of agricultural chemicals. The derivative has a structure as shown in a general formula (I), in the formula, n is an integer from 1 to 5, and R1, R2, R3 and R4 are specific substituent groups. Through a molecular hybridization strategy, the (-)-menthol, the N-fatty acyl piperazine bridge and the nicotinamide fragment are covalently linked, and a compound with a brand new structure is created. A biological activity test shows that the series of compounds have remarkable inhibitory activity on various plant pathogenic fungi, especially sclerotinia sclerotiorum and valsa mali of apple trees, and are low in cytotoxicity. Molecular docking research proves that the action target of the gene is succinate dehydrogenase (SDH). The invention provides a valuable leading structure for developing novel SDHI bactericides.

The invention discloses application of carnosic acid as an agricultural bactericide and a synergist in plant diseases and a composition of the carnosic acid, and the carnosic acid has a remarkable inhibition effect on colletotrichum sorghum and is superior to a positive control drug azoxystrobin. In addition, carnosic acid is used as a synergist and is compounded with a QoI bactericide capable of inhibiting mitochondrial respiration and / or an SDHI succinate dehydrogenase inhibitor, so that the bactericidal activity can be remarkably improved. The antibacterial effect of the bactericidal composition is greatly improved by compounding the bactericidal composition with azoxystrobin, and the anti-colletotrichum sorghum result shows that when the compounding ratio of azoxystrobin to carnosic acid is 1: 4, the synergistic coefficient SR value is 1.637, and the bactericidal composition has a remarkable synergistic effect.

The application belongs to the technical field of pesticide preparation processing, and particularly relates to application of a dispersant composition in inhibiting crystal growth of a pesticide suspension preparation and a pesticide suspension preparation. The application provides application of a dispersant composition in inhibiting crystal growth of a pesticide suspension preparation, wherein the dispersant composition comprises a polycarboxylate dispersant and a mixed high-molecular dispersant containing sulfonic acid groups and carboxyl groups; and a fungicide component in the pesticide suspension preparation is a succinate dehydrogenase inhibitor. The polycarboxylate dispersant and the mixed high-molecular dispersant containing sulfonic acid groups and carboxyl groups are combined, which can effectively inhibit the growth of succinate dehydrogenase inhibitor crystals in the pesticide suspension preparation, and has the effects of viscosity reduction and anti-paste of the pesticide suspension preparation, so that the pesticide suspension preparation can be free of crystal precipitation at variable temperatures, has good fluidity, and has high cold and hot storage stability.

A combination comprising a multi-site contact fungicide, a succinate dehydrogenase inhibitor fungicide and a second systemic fungicide and a method using the same.

The invention discloses a succinate dehydrogenase mutant and application thereof, and belongs to the technical field of gene engineering. The succinate dehydrogenase mutant is obtained by mutation on the basis of an amino acid sequence as shown in SEQ ID NO.1, and the mutation site of the succinate dehydrogenase mutant is selected from at least one of the 117th site, the 119th site, the 134 site, the 256th site and the 402th site. The mutant has L-aspartic acid oxidase activity and shows high catalytic activity and specificity on aspartic acid when being applied to production of quinolinic acid, the catalytic efficiency of the mutant is superior to that of natural L-aspartic acid oxidase, and meanwhile, the substrate inhibition effect of the natural L-aspartic acid oxidase can be avoided; and the high activity can still be maintained under the high NAD < + > concentration. Therefore, by utilizing the mutant and the quinolinic acid synthesis method, the synthesis efficiency of the quinolinic acid can be remarkably improved, and the mutant and the quinolinic acid synthesis method have a good industrial application prospect.

Disclosed are five fungicidal compositions and uses thereof in prevention and treatment of Fusarium diseases of crops, belonging to the technical field of plant disease prevention and treatment. The active ingredients of the compositions comprise a compound ZJS178 of formula (I) and a triazole fungicide, or a compound ZJS178 and a succinate dehydrogenase inhibitor fungicide, or a compound ZJS178 and a pyrrole fungicide, or a compound ZJS178 and an imidazole fungicide, or a compound ZJS178 and a strobilurin fungicide. The compositions of the present invention have a compounded synergistic effect and no cross-resistance, can significantly improve the fungicidal activity and efficacy, are beneficial for overcoming and delaying the resistance of pathogens, have good crop safety, and meet the requirements of pesticide quantity reduction and efficacy increase.

The application discloses a molecular marker of citrus full mite resistance to ethyl acetazolamide and application thereof, and the molecular marker is SNP mutation A269G and / or T270A of succinate dehydrogenase SDHD subunit of the citrus full mite. A detection method of the molecular marker of the citrus full mite resistance to ethyl acetazolamide is also disclosed, total DNA of the citrus full mite to be detected is extracted, PCR amplification is carried out with the total DNA as a template, a succinate dehydrogenase SDHD subunit gene fragment containing a mutation region is obtained, and the obtained gene fragment is subjected to sequence comparison with the molecular marker, so that the resistance of the citrus full mite to be detected to ethyl acetazolamide can be judged. The molecular marker and the detection method can simultaneously detect a large number of samples, can quickly and effectively detect whether the resistance SNP molecular marker and the mutation proportion exist in a field population of the citrus full mite, and can evaluate the mutation frequency of the corresponding site in a certain geographical population.

The invention relates to the technical field of bioengineering, in particular to a recombinant halophilic bacterium for producing PHBV by using acetate as a single carbon source and application of the recombinant halophilic bacterium. The expression and / or activity of 2-methyl citrate synthetase and succinate dehydrogenase assembly factor 2 of the recombinant halophilic bacteria provided by the invention are reduced, 3-hydroxyvaleric acid synthetic genes scpA and scpB are overexpressed, an ADP-dependent acetyl coenzyme A synthetase gene acd is overexpressed, and a heterologous monocarboxylic acid transporter gene mctC is expressed. The strain can generate PHBV without adding precursors such as propionic acid and valeric acid, acetic acid or acetate can be used as a sole carbon source to produce PHBV, the yield is close to that when glucose is used as a carbon source, and the problems that the added precursor substances have certain toxicity, so that the growth of thalli is influenced, and the yield is reduced are solved; environmental pollution caused by addition of precursor substances is avoided, and meanwhile, the fermentation production cost of the PHBV is effectively reduced.

The invention discloses a bactericide and device for improving wheat stem rot resistance in the field of agricultural biocides, and the bactericide comprises the following components in parts by weight: 10-15 parts of magnolol, 30-50 parts of carvacrol and 1 part of ethapyroxad, and the bactericide comprises the following components in parts by weight: 10-15 parts of magnolol, 30-50 parts of carvacrol and 1 part of ethapyroxad. And the bactericide comprises the following components in parts by weight: 15 parts of magnolol, 40 parts of carvacrol and 1 part of ethapyroxad, or 10 parts of magnolol, 30 parts of carvacrol and 1 part of ethapyroxad. The biological activity of the bactericide and the affinity of succinate dehydrogenase are improved by modifying ethane on amino nitrogen of pydiflumetofen, the inhibition effect and stability are improved by mixing natural plant phenol, meanwhile, environmental pollution is reduced, and the mutation speed of pathogenic bacteria is delayed.

Five fungicidal compositions and their use in controlling Fusarium diseases in crops are disclosed, belonging to the field of plant disease control technology. The active ingredients of the compositions include the compound ZJS178 having the structural formula shown in formula (I) and a triazole fungicide, or the compound ZJS178 and a succinate dehydrogenase inhibitor fungicide, or the compound ZJS178 and a pyrrole fungicide, or the compound ZJS178 and an imidazole fungicide, or the compound ZJS178 and a strobilurin fungicide. The compositions of the present invention have a multi-component synergistic effect, are free of cross-resistance, and can significantly improve fungicidal activity and control efficacy, are advantageous in resolving and delaying resistance in disease fungi, are safe for crops, and meet the requirements of reducing the amount of pesticide application and improving efficiency.

The invention discloses a succinate dehydrogenase mutant and application thereof in increasing the yield of glutamic acid, and belongs to the technical field of microorganisms. The technical problem to be solved by the invention is how to improve the glutamic acid yield of microorganisms. In order to solve the technical problem, the protein provided by the invention comprises a protein obtained by mutating the 133rd amino acid of succinate dehydrogenase from arginine (R) to cysteine (C), the protein may be a protein comprising an amino acid sequence of SEQ ID NO: 4. The invention provides the succinate dehydrogenase mutant (SdhAR133C) capable of improving the glutamic acid yield in a target microorganism, and a recombinant bacterium verification result shows that compared with an original target microorganism, the glutamic acid yield of recombinant corynebacterium glutamicum expressing the succinate dehydrogenase mutant (SdhAR133C) is remarkably improved, and an unexpected technical effect is achieved.

The invention provides a propionic acid engineering bacterium as well as a construction method and application thereof. The strain is obtained by taking escherichia coli E.coli W3110 as an original strain through a gene modification method: a Lac l repressor protein gene lacl, a succinate dehydrogenase gene sdhA and an isocitrate lyase inhibitory factor gene iclR are knocked out from a genome, and the escherichia coli E.coli W3110 is obtained through a gene modification method. The method comprises the following steps of: replacing a promoter of methylmalonyl-coenzyme A carboxylase scpA on a genome with a Ptrc promoter, overexpressing a pTrc99a-RM25-1795-1792 plasmid in a constructed strain, knocking out a propionate-coenzyme A synthetase gene prpE, knocking out acs and prpC genes, and carrying out gene knockout; the operation for producing propionic acid by fermentation of the strain is simple, propionic acid can be directly synthesized by using a cheap carbon source, the yield reaches 8.53 g / L after 24 hours through fermentation culture in a 500mL shake flask, and the strain has a very good industrial application prospect.

The invention discloses a high-safety N-[(1-substituted aryl)-alpha-substituted alkyl] formamide compound as well as a preparation method and application thereof in prevention and treatment of plant fungal diseases, enzyme activity and fusarium graminearum in-vitro bacteriostatic activity tests show that the small molecule has efficient bactericidal activity, has no inhibitory effect on human sources, and has a good application prospect. The purposes of specificity and safety are achieved, the configuration of the small molecules is analyzed and confirmed through a cryoelectron microscope structure, and it is found that the small molecules with the R configuration are combined with the fusarium graminearum succinate dehydrogenase.

Disclosed is a composition comprising (a1) a succinate dehydrogenase inhibitor (SDHI) and (a2) a picolinamide.Also disclosed is a method for controlling plant diseases caused by fungal plant pathogens comprising applying to the plant or portion thereof, or to the plant seed, a fungicidally effective amount of a composition comprising (a1) a succinate dehydrogenase inhibitor (SDHI) and (a2) a picolinamide.

The invention discloses an N-pyrazole-4-yl benzamide derivative of a diphenyl ether fragment containing a halogen-mixed substituent group and a preparation method and application thereof, and relates to the field of pesticide bactericides, and the structural general formula is as shown in formula (I). A bacteriostatic activity test is carried out on plant pathogenic fungi by using the pyrazole amide derivative disclosed by the invention, and a test result shows that the compounds have a good bacteriostatic effect on the plant pathogenic fungi such as pyricularia oryzae, fusarium graminearum, cucumber target leaf spot bacteria, rhizoctonia solani and botrytis cinerea; the invention provides a direction for further development of a novel succinate dehydrogenase inhibitor.

Compositions comprising (a1) a succinate dehydrogenase inhibitor (SDHI) and (a2) a picolinamide are disclosed. Also disclosed is a method for controlling plant disease caused by a fungal plant pathogen comprising applying to the plant or part thereof, or to the plant seed, a fungicidally effective amount of a composition comprising (a1) a succinate dehydrogenase inhibitor (SDHI) and (a2) picolinamide.

Pesticidal mixtures comprising as active compounds 1) 1-[(1RS)-1,2-dimethylpropyl]-N-ethyl-5-methyl-N-pyridazin-4-yl-1H-pyrazole-4-carboxamide and 2) at least one further compound B selected from B.1) Succinate dehydrogenase inhibitors as defined in the specification; B.2) Inhibitors of oxysterol binding protein as defined in the specification; B.3) Inhibitors of complex Ill at Qi site selected from: a) florylpicoxamid, and b) fenpicoxamid; B.4) Strobilurins as defined in the specification; B.5) Inhibitors of C14-demethylase in sterol biosynthesis as defined in the specification; B.6) Inhibitors of cell division and cytoskeleton as defined in the specification, B.7) Other compounds of unknown mode of action as defined in the specification; and B.8) N-[[2-fluoro-4-[(2S,3S)-2-hydroxy-3-(3,4,5-trichlorophenyl)-3-(trifluoromethyl)pyrrolidin-1-yl]phenyl]methyl]cyclopropanecarboxamide; wherein component 1) and component 2) are present in a weight ratio of from 1000:1 to 1:1000; methods and use of these mixtures for combating invertebrate pests such as insects, arachnids, nematodes and / or harmful fungi in and on plants, and for protecting such plants being infested with pests and / or harmful fungi.

The application discloses a diflunisal derivative, a preparation method and application thereof, and belongs to the technical field of agricultural fungicides. The preparation method comprises the following steps: mixing 2', 4'-difluoro-4-hydroxy-[1, 1'-biphenyl]-3-carboxylic acid, a condensing agent and an organic base in an ice bath and an inert organic solvent, and activating for 10-30 minutes; then adding an amine compound with a general formula of R-NH2, and stirring and reacting at room temperature for 20-28 hours; after the reaction is completed, extraction, washing, drying, concentration and column chromatography purification are performed, so that the target diflunisal derivative is obtained. The compound disclosed by the application has a novel structure, shows excellent inhibitory activity on cucumber botrytis cinerea, rice pellicularia sasakii, brassica rapa sclerotium, wheat fusarium graminearum and other plant pathogenic fungi, can be used as an active ingredient for preparing an agricultural fungicide or a fungicide composition, and provides a new lead structure for developing a new succinate dehydrogenase inhibitor fungicide.

The invention discloses application of a succinate dehydrogenase mutant in glutamic acid production, and belongs to the technical field of genetic engineering. In order to improve the glutamic acid yield of microorganisms, the invention provides a succinate dehydrogenase mutant, and the succinate dehydrogenase mutant at least comprises protein obtained by mutation of the 301st amino acid residue site of succinate dehydrogenase. Recombinant bacterium verification results show that compared with original target microorganisms, the glutamic acid yield of recombinant corynebacterium glutamicum expressing the succinate dehydrogenase mutant or overexpressing the succinate dehydrogenase is remarkably increased, and an unexpected technical effect is achieved.

The present application discloses a succinate dehydrogenase mutant and its application in increasing glutamate production, belonging to the field of microbial technology. The technical problem to be solved by the present application is: how to increase the glutamate production of microorganisms. To solve the above technical problem, the present application provides the following protein, which comprises a protein obtained by mutating the 133rd amino acid of succinate dehydrogenase from arginine (R) to cysteine (C); the protein may be a protein comprising the amino acid sequence of SEQ ID NO: 4. The present application provides a succinate dehydrogenase mutant (SdhA) with the ability to increase the glutamate production in a target microorganism. R133C ), the verification results of the recombinant bacteria showed that compared with the original target microorganism, the expression of the succinate dehydrogenase mutant (SdhA R133C )'s recombinant Corynebacterium glutamicum significantly increased its glutamate production, achieving unexpected technical results.

The present application relates to the field of microbial technology, in particular to succinate dehydrogenase mutant, recombinant microorganism and its preparation method and application. The present application finds that the yield and conversion rate of products such as lysine taking aspartic acid as a synthetic precursor can be significantly improved by reducing the expression and enzyme activity of succinate dehydrogenase. The succinate dehydrogenase mutant provided by the present application can weaken succinate dehydrogenase, promote the accumulation of products such as lysine taking aspartic acid as a synthetic precursor, and the yield and conversion rate of lysine of the recombinant microorganism expressing the mutant are significantly improved compared with the original strain. The mutant and the recombinant microorganism provide an advantageous modification target, mutant and strain resource for the breeding of aspartic acid, its derivatives and aspartic acid family amino acid production strains.

The application discloses a method for producing fumaric acid by metabolically engineering escherichia coli, and belongs to the fermentation field. In the escherichia coli E.coli FMME-N-5, fumarase gene, fumarate reductase gene, aspartase gene and lactate dehydrogenase gene are knocked out, and phosphoenolpyruvate carboxylase gene and succinate dehydrogenase coding gene are overexpressed. The obtained strain E.coli FMME-N-5 (△fumB△frdBC△aspA△ldhA)-ppc-sdhCDAB is used for fermenting and producing fumaric acid. In a 7.5L fermenter, the fermentation lasts for 65h, the yield of fumaric acid reaches 45g / L, the production intensity is 0.69g / L / h, the yield of fumaric acid to glucose is 0.52g / g, the byproduct formic acid does not accumulate, the accumulation amounts of succinic acid, lactic acid and acetic acid are all lower than 1g / L, and the method has certain industrial production potential.

Provided herein is a method of treating a disease, disorder or condition associated with succinate dehydrogenase- (SDH-) deficiency with a multi-targeted tyrosine kinase inhibitor (TKI). Also provided herein is a method of treating a disease with the combination of multi-targeted TKI with Bcl-2 inhibitor. Further provided herein is a method of treating a disease, disorder or condition associated with abnormal lipid metabolism with a multi-targeted tyrosine kinase inhibitor (TKI).

Pesticidal mixtures comprising as active compounds 1) 1-[(1RS)-1,2-dimethylpropyl]-N-ethyl-5-methyl-N-pyridazin-4-yl-1H-pyrazole-4-carboxamide and 2) at least one further compound B selected from B.1) Succinate dehydrogenase inhibitors as defined in the specification; B.2) Inhibitors of oxysterol binding protein as defined in the specification; B.3) Inhibitors of complex III at Qi site selected from: a) florylpicoxamid, and b) fenpicox-amid; B.4) Strobilurins as defined in the specification; B.5) Inhibitors of C14-demethylase in sterol biosynthesis as defined in the specification; B.6) Inhibitors of cell division and cytoskeleton as defined in the specification, B.7) Other compounds of unknown mode of action as defined in the specification; and B.8) N-[[2-fluoro-4-[(2S,3S)-2-hydroxy-3-(3,4,5-trichlorophenyl)-3-(trifluoromethyl)pyrrolidin-1-yl]phenyl]methyl]cyclopropanecarboxamide; wherein component 1) and component 2) are present in a weight ratio of from 1000:1 to 1:1000; methods and use of these mixtures for combating invertebrate pests such as insects, arachnids, nematodes and / or harmful fungi in and on plants, and for protecting such plants being infested with pests and / or harmful fungi.

The present invention relates to the use of a fungicide selected from the group of phosphonates, in particular Fosetyl-Aluminium, either alone or in combination with a fungicide selected from the group of succinate dehydrogenase inhibitors, in particular Fluopyram for reducing the fitness costs associated with infections of scab resistant and scab susceptible apple plant varieties with Venturia inaequalis. The invention further relates to the use of said fungicides to delay the leaf senescence process in apple.