43 results about "Succinic dehydrogenase" patented technology

The instant invention is drawn to the methods and compositions necessary to provide recombinant proteins with a substantially reduced or eliminated content of norleucine or other non-standard amino acids. Various embodiments of the invention provide for the substantial elimination of the incorporation of non-standard amino acids into recombinant proteins by the co-expression or enhanced expression of a protein (or the enzymatically active portion thereof) capable of degrading norleucine or other non-standard amino acids, including norvaline, beta-methylnorleucine, and homoisoleucine. In certain particular embodiments of the invention, the norleucine is degraded by a glutamate dehydrogenase, a leucine dehydrogenase, a valine dehydrogenase, a phenylalanine dehydrogenase, a glutamate/leucine/phenylalanine/valine dehydrogenase, or an opine dehydrogenase. Also provided are the cells and DNA constructs for carrying out these methods.

The present invention discloses a bactericidal composition containing benziothiazolinone and succinic dehydrogenase inhibitor. The composition comprises two active components, a component A and a component B; the component A is benziothiazolinone, and the component B is a succinic dehydrogenase inhibitor fungicide selected from the group consisting of thifluzamide, boscalid, Penthiopyrad, Isopyrazam, biphenyl penthiopyrad, fluorine myclobutanil amide, Fluxapyroxad, penflufen, fluopyram or Benzovindiflupyr. The weight ratio of the component A and component B is 1:50-50:1, preferably 1:10-10:1. The composition can be prepared into a wettable powder, a water dispersion granule, a suspending agent and an emulsion in water for prevention and control of wheat rust, and downy mildew and epidemic diseases of vegetables, melons and fruits.

The invention discloses an allele of a succinate dehydrogenase B subunit gene sdHB of Corynespora cassiicola and a use thereof. The invention provides a protein. The protein is obtained through mutation of a succinate dehydrogenase B subunit amino acid I at 280th site in the amino acid sequence into V, wherein other amino acid residues are not changed. Novel unreported allele mutation is found in the Corynespora cassiicola group collected in the Liaoning province and can cause high resistance of the strain to fluopyram. The mutation has the important guiding significance for detection of SDHIs resistance in the field and the later drug resistance management.

The invention discloses a method for fast identifying drug resistance of corynespora cassiicola on fluopyram and a special primer pair. The invention provides application of a substance for detecting succinic dehydrogenase B subunit coding gene A1020G site basic group in the corynespora cassiicola genome to the detection of the resistance of the corynespora cassiicola resistance; the A1020G site is the 1020-th site in the succinic dehydrogenase B subunit coding gene; the basic group of the A1020G site is A or G. The method for detecting the point mutation of the drug resistance of the corynespora cassiicola on fluopyram provided by the invention has the advantages that the method can be used for fast and sensitively detecting the resistance occurring and development dynamic state of the field corynespora cassiicola on fluopyram so that the prevention and control strategy on the cucumber target leaf spot diseases can be timely regulated; the further development of the drug resistance can be delayed and controlled.

The invention belongs to the field of medical technology, and specifically relates to an application of escherichia coli succinodehydrogenase iron-sulfur protein SdhB. According to active immunization and passive immunization tests, the escherichia coli succinodehydrogenase iron-sulfur protein SdhB and the corresponding antibody have an obvious immune protection effect on the infection of clinical pathogenic bacteria of escherichia coli. Therefore, the escherichia coli succinodehydrogenase iron-sulfur protein SdhB and the corresponding antibody can be used for preparing a medicine for preventing and treating diseases caused by the infection of clinical pathogenic bacteria of animal escherichia coli. The medicine does not generate drug resistance and can effectively prevent and treat the diseases caused by the infection of clinical pathogenic bacteria of escherichia coli.

The invention discloses a method for increasing the yield of 5-aminolevulinic acid synthesized by corynebacterium glutamicum, and belongs to the technical field of bioengineering. According to the method, the construction of the recombinant corynebacterium glutamicum for knocking out alpha-ketoglutaric acid inhibitory protein and succinate dehydrogenase and overexpressing 5-aminolevulinic acid synthetase is successfully realized. A 5-L fermentation tank batch fermentation strategy is adopted, fermentation conditions are optimized, finally, recombinant corynebacterium glutamicum (C. glutamicum) 13032/delta odhI delta sdhA/pXMJ19-hemA is fermented for 84 h, 25.05 g/L of 5-ALA is synthesized within 48 h under the condition that glycine with the final concentration of 4 g/L is added, the productivity is 0.52 g/L/h, and at the moment, the sugar-acid conversion rate reaches 16.79%. The high yield of the 5-aminolevulinic acid is realized, and the highest yield of one-step fermentation starting from glucose as a carbon source to the current is obtained.

The invention belongs to the technical field of waste water processing, and especially relates to a method for obtaining plant capable of efficiently absorbing nitrate. The method comprises the following steps: 1) using a potassium nitrate solution for immersing the plant seeds under dark condition and vibrating by a shaking table; 2) transferring the seeds in the step 1) to a watch glass with wet filter paper; 3) after 7 days, transferring the seeds in the step 2) to a flat vessel with the wet filter paper; and 4) placing the seeds in the step 3) in a warm room with irritation of a fluorescent lamp. Compared with a blank sample, the total phenols substance in the plant is increased, and total antioxidation substance activity is increased; activity of nitrate reductase, glucose-6-phosphate dehydrogenase and succinic dehydrogenase is increased, content of total proline is increased, activity of proline dehydrogenase is increased; the activity of superoxide dismutase, catalase and guaiacol oxidase is increased, and the above biochemical indexes are effective when waste water containing ammonia nitrogen and nitrate is processed.

The invention provides a TET enzyme activity determination method. The TET enzyme activity determination method is characterized by comprising the following steps: mixing 5mC DNA with a TET enzyme to be detected, and incubating; adding SDH and DCPIP into a reaction system for reaction; adding trichloroacetic acid to terminate the reaction; measuring the absorption peak value of the reaction liquid at 600nm by a microplate reader, wherein the higher the peak value is, the stronger the enzyme activity is. The invention also discloses an application of the combination of SDH and DCPIP in determination of TET enzyme activity, and a high-throughput screening method of a TET enzyme activity small molecule activator or inhibitor. The invention provides a simple and rapid TET enzyme activity determination method, a TET enzyme oxidation reaction product succinate releases reducing hydrogen under the catalysis of succinate dehydrogenase, the reducing hydrogen is captured by DCPIP to generate DCPIPH2 (blue), and a specific absorption peak appears at the wavelength of 600 nm. The whole process of the method only needs four-step operation and one-tube reaction, can be completed within 1 hour, and has the advantages of low cost, large flux, wide application range and high accuracy.

The invention discloses a full-biosynthesis method of malonic acid, and belongs to the technical field of bioengineering. According to the full-biosynthesis method of malonic acid, escherichia coli BL21 (DE3) is taken as a host, a phosphoenolpyruvate carboxylase gene (ppc), a succinate dehydrogenase gene (sdhC), a succinate semialdehyde dehydrogenase gene (yne1) and an asparaginase gene (apa) from the escherichia coli, and a heterologous gene aspartic acid-alpha-dehydrogenase gene (panD) from Corynebacterium glutamicum are subjected to modular overexpression, and a heterologous gene beta-alanine pyruvate transaminase gene (pa0123) from pseudomonas aeruginosa constructs a full biosynthetic pathway of the malonic acid, so that the engineering bacteria successfully accumulate the malonic acid. Biological manufacturing of the malonic acid and a precursor thereof has the advantages of low pollution, high product quality and the like, and has a great development prospect.

The invention discloses a target drug for treating thyroid cancer and applications thereof. Through long term research, for the first time, the inventor finds that aluminum sulfate can recognize the normal tissues and cancer tissues of human body, can change the physiological features of cancer cells, can inhibit cancer cells from secreting multiple proteases, and inhibits the metabolism of mitochondria of cancer cells. The drug can treat cancers. The drug can be combined with the DNA of cancer cells to promote the DNA cleavage, glycoprotein on the cell surface can be rapidly astringed and agglomerated, the structure of proteins on the cell surface is effectively changed, the signal channel is obstructed, the cell microstructure is changed, the drug combines with succinodehydrogenase in cell mitochondria, the proliferation and migration of cancer cells are controlled effectively, and the cancer cell inhibition rate is as high as 99.8 to 99.9% or even 100%. The side effect of aluminum sulfate is small, the safety is high, the oral administration has the advantages of low dosage, rapid effect, and short treatment course; the tumor size can be reduced rapidly, the apoptosis is induced, the proliferation is inhibited, finally the cancer cells fall off in fragments, and the drug has an important meaning for thyroid cancer treatment.

The invention discloses a target drug for treating lung cancer and applications thereof. Through long term research, for the first time, the inventor finds that aluminum sulfate can recognize the normal tissues and cancer tissues of human body, can change the physiological features of cancer cells, can inhibit cancer cells from secreting multiple proteases, and inhibits the metabolism of mitochondria of cancer cells. The drug can treat cancers. The drug can be combined with the DNA of cancer cells to promote the DNA cleavage, glycoprotein on the cell surface can be rapidly astringed and agglomerated, the structure of proteins on the cell surface is effectively changed, the signal channel is obstructed, the cell microstructure is changed, the drug combines with succinodehydrogenase in cellmitochondria, the proliferation and migration of cancer cells are controlled effectively, and the cancer cell inhibition rate is as high as 99.8 to 99.9% or even 100%. The side effect of aluminum sulfate is small, the safety is high, the oral administration has the advantages of low dosage, rapid effect, and short treatment course; the tumor size can be reduced rapidly, the apoptosis is induced, the proliferation is inhibited, finally the cancer cells fall off in fragments, and the drug has an important meaning for lung cancer treatment.

A combination comprising a multi-site contact fungicide, a succinate dehydrogenase inhibitor fungicide and a second systemic fungicide and a method using the same.

The invention relates to a bactericidal mixture containing a succinate dehydrogenase inhibitor and application thereof. The bactericidal mixture comprises a fungicidally effective amount of a compoundas shown in a formula I and at least one compound selected from succinate dehydrogenase inhibitors including fluxapyroxad, penflufen, pydiflumetofen and isoflucypram. The bactericidal mixture provides synergistic control of selected plant diseases.

The invention discloses a bactericidal composition containing isotianil and Xinjunan acetate, and relates to the field of pesticides. The active ingredients of the bactericidal composition are isotianil, Xinjunan acetate and a bactericidal assistant, the bactericidal assistant inhibits respiration of pathogenic bacteria by inhibiting succinate dehydrogenase of composite II iron on a bacterial respiration transmission chain through an amide group, so that the sterilization effect is achieved; fluorine atoms are small in size and can be taken into the body as hydride by organisms, so the action of the amide group can be further promoted; due to the fact that introduced fluorine-containing groups have the characteristics of super acid, normal electron transfer of harmful substances is destroyed, and the sterilization effect is achieved; and fluorocarbon bonds are large in bond energy, high in stability and not prone to decomposition in the acting process in organisms, so the long-acting sterilization effect can be achieved, and the bactericidal composition has the excellent sterilization effect under reasonable compatibility of the fluorine-containing groups, the rice morbidity is reduced, the rice growth is facilitated, and the rice yield is improved.

The invention discloses a drug for targeted therapy of breast cancer and application thereof. It is discovered for the first time through long-term researches that aluminum sulfate is capable of identifying human normal tissues from cancer tissues, changing physiological characteristics of cancer cells, inhibiting the cancer cells from secreting various protein hydrolases and inhibiting the metabolic function of mitochondria of the cancer cells, thereby arriving at anti-tumor effect; the drug can be combined to DNA of cancer cells to promote their lysis, can quickly converge and gather cell surface glycoproteins, effectively change the surface protein structure of the cells, block the signal pathways and change the microstructure of the cells, and can combine with succinic dehydrogenase inthe mitochondria of the cells to effectively control the proliferation and metastasis of the cancer cells, and the inhibition rate for cancer cells is up to 99.8-99.9%, and even 100%; aluminum sulfate has small side effects and high safety, features low dosage, high acting speed, short treatment term and the like in treating breast cancer, can quickly narrow tumors, induce death and inhibit proliferation so as to finally separate in fragments, and is important for human to overcome breast cancer.

The invention discloses a method for detecting the drug resistance of Corynespora cassiicoa to SDHI fungicides by using MAS-PCR technology and a used primer composition. Specific primers D95E-R, H105R-R, and G109V-R in the primer composition are reverse primers designed targeted to mutation sites D95E, H105R and G109V of succinate dehydrogenase D subunit of Corynespora cassiicoa resistant to boscalid correspondingly, corresponding sequences are at the mutation sites, and a 3'end base is specially designed, so that the specificity of the primers is improved. The method for detecting or auxiliary detecting the drug resistance of Corynespora cassiicoa to the SDHI fungicides can simultaneously detect the three mutation types of D95E, H105R and G109V in a PCR reaction tube, compared with otherdetection methods that can only detect a single mutation, the method has the advantages of time-saving, labor-saving, fast and high-throughput, and a large number of samples can be accurately detected.

The invention discloses a drug for treating gastric cancer and its application. Through long-term study, an inventor finds that aluminum sulfate can distinguish and identify normal tissues and cancertissues of a human body, changes physiological feature of cancer cells, inhibits cancer cells to secrete multiple protein hydrolases, and inhibit the metabolism of cancer cell mitochondria for the first time, thus the anti-tumor effect is realized; the drug can be combined to DNA of cancer cell to promote its splitting, rapidly and strongly restrain and gather glycoprotein of cell surface, effectively change protein structure of the cell structure, stop signal channel, and change the cell micro-structure; besides, the drug can be combined with succinodehydrogenase in the cell mitochondria, thus the proliferation and transfer of the cancer cell are effectively controlled, and the inhibition rate of the cancer cell is up to 99.8-99.9% and even 100%. The aluminum sulfate is small in side effect and high in safety; the drug for treating gastric cancer by orally taking has the advantages of being trace in dosage, quick in effect, and short in treatment course; the drug can rapidly decreasemyoma volume, induce apoptosis, and suppress proliferation; finally, the drug is fragmented and droped, and has important realistic meaning for human in attacking the gastric cancer.

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a rehmannia glutinosa libosch. root decoction piece and a preparation method thereof. According to the invention, a vitamin C aqueous solution is adopted for spraying a fresh rootstock slice of rehmannia glutinosa libosch. to prepare the rehmannia glutinosa libosch. root decoction piece; andthe prepared rehmannia glutinosa libosch. root decoction piece has catalpol content higher than or equal to 79.35 mg.g<-1>, has the effect of removing heat to promote salivation equivalent to that offresh rehmannia glutinosa libosch. root, and keeps the colour of the fresh rehmannia glutinosa libosch. root. According to experiments, the rehmannia glutinosa libosch. root decoction piece can obviously improve content of Na<+>-K<+>-ATP enzyme, Ca<2+>-Mg<2+>-ATP enzyme, lactic dehydrogenase (LDH), succinic dehydrogenase (SDH), interleukin-6(IL-6) and interleukin-8(IL-8) in yeast and LPS (Lipopolysaccharide) induced heating model rat serum, has good antipyretic effect, and has treatment effect equivalent to that of fresh rehmannia glutinosa libosch. root juice. The preparation method involvedin the invention is simple and convenient in operation, is high in efficiency, is stable in process, is low in cost, is convenient in storage and transportation, and is suitable for industrial production.

The invention discloses a rapid determination method of vitality of spleen aminopeptide oral lyophilized powder. When the spleen aminopeptide oral lyophilized powder and splenic lymphocytes of healthymice are incubated together, immunocompetence of the spleen aminopeptide oral lyophilized powder can be adopted to the splenic lymphocytes of the mice to make the splenic lymphocytes of the mice be sensitized lymphocytes. The sensitized lymphocytes release leukocyte adhesion inhibitors, so that the leukocyte adhesion inhibition index is increased. Therefore, the vitality of a finished product canbe verified by determining the leukocyte adhesion inhibition index. According to the rapid determination method, methyl thiazolyl tetrazolium (MTT) is introduced since that the MTT can be reduced toa blue-violet compound (Formazan) by succinodehydrogenase in living cell mitochondria, the number of the reduction product is proportional to the number of cells within a certain range, the Formazan deposited in the cells is dissolved through dimethyl sulfoxide, the A value of the 490 nm wavelength is determined through an enzyme-linked detector, the value is substituted into a formula, and thus the adhesion inhibition index can be solved. The rapid determination method is simple, and detection data are more accurate.

The invention discloses application of an SDHC subunit genetically differentiated by fusarium as a drug target resource in drug research and development. The application shows that SDHC1 gene regulation and control on drug resistance of a succinate dehydrogenase inhibitor bactericide and SDHC2 gene regulation and control on sensitivity of the succinate dehydrogenase inhibitor bactericide provide atheoretical basis for revealing drug resistance research of the fusarium on a succinate dehydrogenase inhibitor and action mechanism research of the succinate dehydrogenase inhibitor on the fusarium,and provide important theoretical data for drug research and development with the SDHC1 gene and/or the SDHC2 gene as a drug target.

The invention relates to the field of pesticide bactericides, and discloses an amide compound containing 3-trifluoromethylpyridine, a preparation method and applications thereof, and a bactericide, wherein the amide compound containing 3-trifluoromethylpyridine has a structure represented by a formula (1). According to the invention, by introducing the structure containing 3-trifluoromethylpyridine and the diphenyl ether fragment, the amide compound containing 3-trifluoromethylpyridine with wide activity is designed, and can be used as a completely-new succinate dehydrogenase inhibitor or bactericide.

The invention provides compounds that are inhibitors or covalent modifiers of succinate dehydrogenase subunit B (SDHB) and/or inhibitors of apoptosis, and pharmaceutically acceptable salts, hydrates and stereoisomers thereof. The compounds are employed in pharmaceutical compositions, and methods of making and use, including treating a person in need thereof with an effective amount of the compound or composition.

The invention relates to the use of succinate dehydrogenase SDH inhibitors (SDHIs), in particular bixafen, penflufen or fluopyram for controlling wood diseases in grape, to a method for treating plants or plant parts for controlling wood diseases in grape and to a method for controlling wood diseases in grape plants and plant parts, and in plants which grow from the seedlings, grafts and cuttings, by treating them with SDHIs.

The invention relates to a succinate dehydrogenase inhibitor or a prodrug and/or a pharmaceutically acceptable salt thereof for use in the treatment or prevention of reperfusion injury, such as ischemia-reperfusion injury, by inhibiting the accumulation of succinate, wherein the inhibitor or prodrug and/or pharmaceutically acceptable salt thereof is a cell-permeable reversible inhibitor of succinate dehydrogenase.