13 results about "Succinic dehydrogenase" patented technology

The invention discloses application of hydrogen-rich water in preparation of a drug for treating Duchenne type muscular dystrophy, and particularly relates to the technical field of medicines.The random control experiment is adopted, homologous c57 mice serve as a normal control group, mdx mice are randomly divided into a model group and a hydrogen-rich water group, and the exercise ability is evaluated through a four-limb holding power test and a rotating bar test; detecting mouse creatine kinase by an enzyme-linked immunosorbent assay; carrying out hematoxylin-eosin, NADH-tetrazole reductase and improved Goori three-color dyeing, and carrying out cytochrome C oxidase, succinate dehydrogenase and SDH-COX combined dyeing to observe the pathological change of the skeletal muscle tissue; the invention discloses that the muscle injury condition of a Duchenne type muscular dystrophy model mouse is improved by the hydrogen-rich water through a mitochondrial protection mechanism, and the hydrogen-rich water has a relatively high application prospect and a relatively high economic value.

The invention discloses a (-)-menthol group-containing nicotinamide derivative with antifungal activity as well as a preparation method and application thereof, and relates to the technical field of synthesis of agricultural chemicals. The derivative has a structure as shown in a general formula (I), in the formula, n is an integer from 1 to 5, and R1, R2, R3 and R4 are specific substituent groups. Through a molecular hybridization strategy, the (-)-menthol, the N-fatty acyl piperazine bridge and the nicotinamide fragment are covalently linked, and a compound with a brand new structure is created. A biological activity test shows that the series of compounds have remarkable inhibitory activity on various plant pathogenic fungi, especially sclerotinia sclerotiorum and valsa mali of apple trees, and are low in cytotoxicity. Molecular docking research proves that the action target of the gene is succinate dehydrogenase (SDH). The invention provides a valuable leading structure for developing novel SDHI bactericides.

The application belongs to the technical field of pesticide preparation processing, and particularly relates to application of a dispersant composition in inhibiting crystal growth of a pesticide suspension preparation and a pesticide suspension preparation. The application provides application of a dispersant composition in inhibiting crystal growth of a pesticide suspension preparation, wherein the dispersant composition comprises a polycarboxylate dispersant and a mixed high-molecular dispersant containing sulfonic acid groups and carboxyl groups; and a fungicide component in the pesticide suspension preparation is a succinate dehydrogenase inhibitor. The polycarboxylate dispersant and the mixed high-molecular dispersant containing sulfonic acid groups and carboxyl groups are combined, which can effectively inhibit the growth of succinate dehydrogenase inhibitor crystals in the pesticide suspension preparation, and has the effects of viscosity reduction and anti-paste of the pesticide suspension preparation, so that the pesticide suspension preparation can be free of crystal precipitation at variable temperatures, has good fluidity, and has high cold and hot storage stability.

The invention discloses a succinate dehydrogenase mutant and application thereof, and belongs to the technical field of gene engineering. The succinate dehydrogenase mutant is obtained by mutation on the basis of an amino acid sequence as shown in SEQ ID NO.1, and the mutation site of the succinate dehydrogenase mutant is selected from at least one of the 117th site, the 119th site, the 134 site, the 256th site and the 402th site. The mutant has L-aspartic acid oxidase activity and shows high catalytic activity and specificity on aspartic acid when being applied to production of quinolinic acid, the catalytic efficiency of the mutant is superior to that of natural L-aspartic acid oxidase, and meanwhile, the substrate inhibition effect of the natural L-aspartic acid oxidase can be avoided; and the high activity can still be maintained under the high NAD < + > concentration. Therefore, by utilizing the mutant and the quinolinic acid synthesis method, the synthesis efficiency of the quinolinic acid can be remarkably improved, and the mutant and the quinolinic acid synthesis method have a good industrial application prospect.

The invention relates to the technical field of bioengineering, in particular to a recombinant halophilic bacterium for producing PHBV by using acetate as a single carbon source and application of the recombinant halophilic bacterium. The expression and / or activity of 2-methyl citrate synthetase and succinate dehydrogenase assembly factor 2 of the recombinant halophilic bacteria provided by the invention are reduced, 3-hydroxyvaleric acid synthetic genes scpA and scpB are overexpressed, an ADP-dependent acetyl coenzyme A synthetase gene acd is overexpressed, and a heterologous monocarboxylic acid transporter gene mctC is expressed. The strain can generate PHBV without adding precursors such as propionic acid and valeric acid, acetic acid or acetate can be used as a sole carbon source to produce PHBV, the yield is close to that when glucose is used as a carbon source, and the problems that the added precursor substances have certain toxicity, so that the growth of thalli is influenced, and the yield is reduced are solved; environmental pollution caused by addition of precursor substances is avoided, and meanwhile, the fermentation production cost of the PHBV is effectively reduced.

Disclosed is a composition comprising (a1) a succinate dehydrogenase inhibitor (SDHI) and (a2) a picolinamide.Also disclosed is a method for controlling plant diseases caused by fungal plant pathogens comprising applying to the plant or portion thereof, or to the plant seed, a fungicidally effective amount of a composition comprising (a1) a succinate dehydrogenase inhibitor (SDHI) and (a2) a picolinamide.

The invention discloses an N-pyrazole-4-yl benzamide derivative of a diphenyl ether fragment containing a halogen-mixed substituent group and a preparation method and application thereof, and relates to the field of pesticide bactericides, and the structural general formula is as shown in formula (I). A bacteriostatic activity test is carried out on plant pathogenic fungi by using the pyrazole amide derivative disclosed by the invention, and a test result shows that the compounds have a good bacteriostatic effect on the plant pathogenic fungi such as pyricularia oryzae, fusarium graminearum, cucumber target leaf spot bacteria, rhizoctonia solani and botrytis cinerea; the invention provides a direction for further development of a novel succinate dehydrogenase inhibitor.

The application discloses a diflunisal derivative, a preparation method and application thereof, and belongs to the technical field of agricultural fungicides. The preparation method comprises the following steps: mixing 2', 4'-difluoro-4-hydroxy-[1, 1'-biphenyl]-3-carboxylic acid, a condensing agent and an organic base in an ice bath and an inert organic solvent, and activating for 10-30 minutes; then adding an amine compound with a general formula of R-NH2, and stirring and reacting at room temperature for 20-28 hours; after the reaction is completed, extraction, washing, drying, concentration and column chromatography purification are performed, so that the target diflunisal derivative is obtained. The compound disclosed by the application has a novel structure, shows excellent inhibitory activity on cucumber botrytis cinerea, rice pellicularia sasakii, brassica rapa sclerotium, wheat fusarium graminearum and other plant pathogenic fungi, can be used as an active ingredient for preparing an agricultural fungicide or a fungicide composition, and provides a new lead structure for developing a new succinate dehydrogenase inhibitor fungicide.

The present application relates to the field of microbial technology, in particular to succinate dehydrogenase mutant, recombinant microorganism and its preparation method and application. The present application finds that the yield and conversion rate of products such as lysine taking aspartic acid as a synthetic precursor can be significantly improved by reducing the expression and enzyme activity of succinate dehydrogenase. The succinate dehydrogenase mutant provided by the present application can weaken succinate dehydrogenase, promote the accumulation of products such as lysine taking aspartic acid as a synthetic precursor, and the yield and conversion rate of lysine of the recombinant microorganism expressing the mutant are significantly improved compared with the original strain. The mutant and the recombinant microorganism provide an advantageous modification target, mutant and strain resource for the breeding of aspartic acid, its derivatives and aspartic acid family amino acid production strains.

The invention discloses polypeptide-assisted molybdenum disulfide nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of polymer composite materials. The preparation method comprises the following steps: (1) dissolving a polypeptide polymer with a flexible main chain and a charged side chain in a solvent to prepare a polypeptide polymer solution; (2) mixing molybdenum source molecules, sulfur source molecules and a reducing agent in water to prepare a precursor solution; and (3) adding the polypeptide polymer solution obtained in the step (1) into the precursor solution obtained in the step (2), uniformly mixing, carrying out hydrothermal reaction, cooling, centrifuging, washing and drying to obtain the polypeptide-assisted molybdenum disulfide nano-enzyme. The polypeptide-assisted molybdenum disulfide nano-enzyme shows excellent succinate-like dehydrogenase and peroxidase catalytic activity, can effectively promote algae metabolism and increase the biomass yield, and has good practical value in the fields of biological catalysis, energy conversion and environmental protection.

The present invention concerns an agrochemical composition comprising: - particles of at least one silicate; - one or more fungicide(s) selected from the group consisting of strobilurin fungicides, triazole fungicides, dithio-carbamate fungicides, succinate dehydrogenase inhibitors and biofungicides. The invention also concerns a method for treating a plant wherein a composition as described above is applied onto or next to at least one part of said plant.

The invention discloses an oral medicine containing sodium dehydroacetate, which is used for treating helicobacter pylori infection. In order to solve the problems of antibiotic resistance and gastrointestinal tract microecological disorder caused by the existing quadruple therapy, free molecules of the medicine disclosed by the invention under the gastric acid condition have strong lipophilicity, can efficiently penetrate through a gastric mucous layer and bacterial cell membranes, and can cause bacterial energy depletion death by blocking tricarboxylic acid cycle and energy metabolic centers such as succinate dehydrogenase. Animal experiments prove that the curative effect of reducing Hp colonization is equivalent to or even better than that of quadruple therapy. As a non-antibiotic bacteriostatic agent, the product has the advantages that drug resistance is not easy to generate due to multi-target action, normal intestinal flora is not damaged, flora imbalance and secondary infection are effectively avoided, and the safety is obviously improved.