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Method for identifying tobacco mosaic virus species and its special barcode primers

A technology of tobacco mosaic virus and barcode primers, which is applied in the biological field, can solve the problems that have not yet been developed in biological barcode technology, and achieve the effect of preventing the introduction of viruses

Active Publication Date: 2016-01-13
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of plant viruses, there is no research on biological barcoding technology

Method used

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  • Method for identifying tobacco mosaic virus species and its special barcode primers
  • Method for identifying tobacco mosaic virus species and its special barcode primers
  • Method for identifying tobacco mosaic virus species and its special barcode primers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, be used for the synthesis of the barcode primer set of identification virus species in the tobacco mosaic virus genus

[0028] Taking the representative species of 22 species of Tobacco mosaic virus in Genbank as the virus voucher samples, the conserved region of its sequence was analyzed. Variability designed and synthesized primer sets TobamoF6 and TobamoR6 for the identification of Tobamovirus species, and the length of the amplified product of the barcode primer set was 200bp.

[0029] The nucleotide sequence of TobamoF6 is sequence 1 in the sequence listing, and the nucleotide sequence of TobamoR6 is sequence 2 in sequence listing China.

Embodiment 2

[0030] Embodiment 2, barcode primer set identifies the virus species of Tobamovirus genus

[0031] 1. Identification of Tobacco Mosaic Virus Species by Barcode Primer Set

[0032] In order to verify the versatility of the barcode primer set used to identify Tobamovirus prepared in Example 1, the present embodiment selects from watermelon, pepper, pumpkin, tomato, kidney bean, tobacco and cucumber samples with obvious mosaic symptoms. Four Tobamoviruses (pepper light mottle virus, cucumber green mottle mosaic virus, tobacco mosaic virus and tomato mosaic virus) were evaluated.

[0033]Pepper mild mottle virus (Peppermildmottlevirus): recorded in "Detection of pepper mild mottle virus in pepper seeds. Anhui Agricultural Sciences, 2007, 35(14): 4228-4229.", the public can obtain from the Chinese Academy of Inspection and Quarantine .

[0034] Cucumbergreenmottlemosaicvirus: recorded in "Occurrence of CucumbergreenmottlemosaicvirusonCucurbitaceousPlantsinChina.Plantdisease, 2009...

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Abstract

The invention discloses a method for authenticating the species of tobacco mosaic viruses and a special bar code primer thereof. A bar code primer group provided by the invention consists of Tobamo F6 and Tobamo R6. An experiment shows that by the use of the primer group provided by the invention, a sample for representing the symptom can be amplified to tobacco mosaic virus type viruses by 100 percent, and the sequencing success rate is 100 percent; the method can be used for effectively performing species authentication on the viruses in the species. The special bar code primer can be applied to quarantine of imported plants and products of the imported plants, and virus introduction is prevented; the special bar code primer can be also popularized and applied to a plant protection and detection station, a scientific research institution, an agricultural production enterprise and a peasant household in a large range in China.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying tobacco mosaic virus species and special barcode primers thereof. Background technique [0002] The genus Tobamovirus is a member of Virgaviridae in the order Tymovirales; according to the ninth report on the latest virus classification issued by the International Committee Taxonomy of Viruses (ICTV) in 2012, The virus genus includes Brugmansiamildmottlevirus (BrMMV), cucumber fruit mottlemosaicvirus (CFMMV), cucumber green mottlemosaicvirus (CGMMV), frangipanimosaicvirus (FrMV) , Hibiscus latent Fort Pierce virus (Hibiscus latent Fort Pierce virus, HLFPV), hibiscus latent Singapore virus (Hibiscus latent Singapore virus, HLSV), Kyuri green mottle mosaic virus (KGMMV), old Buda pepper virus (Obudapeppervirus, ObPV), tooth blue ring spot virus ( Odontoglossum ringspotvirus (ORSV), Paprikamildmottlevirus (PaMMV), Peppermildmottlevirus (PMMoV), Rehm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/94
CPCC12Q1/6869C12Q1/70C12Q2563/185C12Q2531/113
Inventor 张永江马荣群辛言言马洁
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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