Bacteriostatic composition of lactobacillus plantarum fermentation liquid and nano zinc oxide

A technology of Lactobacillus plantarum and nano-zinc oxide, which is applied in the field of medicine, can solve the problems that it is difficult to reflect the antibacterial performance and nano-zinc oxide is difficult to dissolve in water, and achieve the effect of enhancing solubility and enhancing antibacterial performance

Active Publication Date: 2014-08-13
安信生物科技(湖南)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that nano-zinc oxide is difficult to dissolve in water so that it is difficult to reflect its antibacterial performance, the inven

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0013] Example 1

[0014] 1. The preparation of Lactobacillus plantarum fermentation broth includes the following steps: Take Lactobacillus plantarum as a starting strain, MRS is a liquid medium, and a single colony of Lactobacillus plantarum (purchased from ATCC No. ATCC 8014) is placed in 5 mL of MRS as a liquid medium. , Incubate at a constant temperature in an anaerobic incubator at 37°C for 24 hours, and the bacteria concentration can be obtained at 10 8 CFU / mL Lactobacillus plantarum fermentation broth.

[0015] 2. Preparation of nano-zinc oxide colloidal solution: Dissolve 0.01 g of nano-zinc oxide produced by Aladdin in 1 mL of distilled water, shake and mix well to obtain a 10 mg / mL nano-zinc oxide colloidal solution.

[0016] 3. The combined antibacterial effect of Lactobacillus plantarum and nano-zinc oxide.

[0017] a Cultivation of bacteria

[0018] Pick a single colony of E. coli O157 on the LB agar medium and place it in 10 mL LB broth medium, culture it in a 37°C incub...

Example Embodiment

[0026] Example 2

[0027] 1. The preparation of Lactobacillus plantarum fermentation broth includes the following steps: the same as in Example 1.

[0028] 2. Preparation of nano zinc oxide colloidal solution: the same as in Example 1.

[0029] 3. The combined antibacterial effect of Lactobacillus plantarum fermentation broth and nano zinc oxide

[0030] a Cultivation of bacteria

[0031] Pick a single colony of Bacillus cereus on the LB agar medium in 5 mL LB broth medium, culture it in a 37 ℃ incubator for 12 h, and then inoculate 1% of the inoculum into 5 mL LB broth for culture In the base, after 5h incubation at 37℃. Take 1 mL of the above bacterial solution and dilute two gradients with 1xPBS water. The final bacterial solution is about 10 8 CFU / mL.

[0032] b The combined effect of Lactobacillus plantarum fermentation broth and nano-zinc oxide on the antibacterial properties

[0033] Take 900μL of the above-diluted bacterial solution in a 1.5mL sterile centrifuge tube, add 100μL...

Example Embodiment

[0039] Example 3

[0040] Preparation of freeze-dried bacterial powder from the fermentation broth of Lactobacillus plantarum: ① Preparation of bacterial cells: The bacterial strains were inoculated into MRS liquid medium at 5% inoculum, and cultured anaerobic in an anaerobic incubator at 37°C for 24 hours. Microscopic examination confirmed that there are no bacteria. ②Preparation of protective agent: pre-prepared trehalose 4.47%, D-sorbitol 13.42%, skimmed milk powder 9.24% were taken out and dissolved in 100mL sterile water, and then placed in a stoppered glass bottle, 121 ℃, sterilized 30 m in. ③On the aseptic operation table, mix the fermentation broth sample of Lactobacillus obtained from fermentation with the sterilized protectant solution and pour it into a sterile petri dish, pre-frozen at -80℃ for 2h, and quickly after the sample is completely frozen Transfer to a freeze-drying machine and freeze-dry for 24 hours, and the final product can be harvested after the water ...

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Abstract

The invention discloses a bacteriostatic composition of a lactobacillus plantarum fermentation liquid and nano zinc oxide, and belongs to the field of medicine. Combined use of the lactobacillus plantarum fermentation liquid and nano zinc oxide in the bacteriostatic composition plays a combined bacteriostatic effect on inhibition of escherichia coli O157 and bacillus cereus. Compared with the prior art, the bacteriostatic composition of the lactobacillus plantarum fermentation liquid and nano zinc oxide solves the problem that nano zinc oxide is difficult to dissolve in water in a conventional bacteriostatic method, and at the same time, with adopting of the two bacteriostatic substances, the bacteriostatic composition reduces the occurrence probability of microbial acid resistance, reduces toxicity to human bodies, and is safer.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a bacteriostatic mixture. Background technique [0002] Inorganic antibacterial agents and their products have become a research and development hotspot in the field of antibacterial materials due to their unique functions such as safe, reliable, stable and durable antibacterial properties, and have generally attracted people's attention and become the main research object of everyone. Among them, nano-zinc oxide is a white solid with good stability, no discoloration at high temperature, no decomposition, low price, and abundant resources. It has become one of the hot spots in the research of inorganic antibacterial agents. It has a wide range of physiological and biochemical functions in the body, and is also called "life element". As a bacteriostatic agent, nano-zinc oxide has attracted widespread attention in the medical field. [0003] Nano-zinc oxide is made into a bacteriostatic a...

Claims

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Application Information

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IPC IPC(8): A61K33/30A61P31/04A61K35/74
CPCY02A50/30
Inventor 许恒毅况慧娟段宏沈骏邴双飞
Owner 安信生物科技(湖南)有限公司
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