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Bacteriostatic composition of lactobacillus plantarum fermentation liquid and nano zinc oxide

A technology of Lactobacillus plantarum and nano-zinc oxide, which is applied in the field of medicine, can solve the problems that it is difficult to reflect the antibacterial performance and nano-zinc oxide is difficult to dissolve in water, and achieve the effect of enhancing solubility and enhancing antibacterial performance

Active Publication Date: 2014-08-13
安信生物科技(湖南)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that nano-zinc oxide is difficult to dissolve in water so that it is difficult to reflect its antibacterial performance, the inventor has conducted a large number of experiments and found that the fermentation liquid of Lactobacillus plantarum can enhance the solubility of nano-zinc oxide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. The preparation of Lactobacillus plantarum fermentation broth comprises the following steps: take Lactobacillus plantarum as the starting strain, MRS as the liquid medium, and connect Lactobacillus plantarum (purchased from ATCC numbering as ATCC 8014) single colony in 5mL of MRS as the liquid medium , in an anaerobic incubator at 37°C for 24 hours at a constant temperature, you can get the bacteria concentration at 10 8 CFU / mL of Lactobacillus plantarum fermentation broth.

[0015] 2. Preparation of nano-zinc oxide colloidal solution: 0.01 g of nano-zinc oxide produced by Aladdin Company was dissolved in 1 mL of distilled water, oscillated and mixed to obtain a 10 mg / mL nano-zinc oxide colloidal solution.

[0016] 3. The combined antibacterial effect of Lactobacillus plantarum and nano zinc oxide.

[0017] a Culture of bacteria

[0018] Pick a single colony of Escherichia coli O157 on LB agar medium and put it in 10mL LB broth medium, culture it in a 37°C incubat...

Embodiment 2

[0027] 1. The preparation of Lactobacillus plantarum fermentation broth comprises the following steps: same as in Example 1.

[0028] 2. Preparation of nano-zinc oxide colloidal solution: same as in Example 1.

[0029] 3. Combined antibacterial effect of Lactobacillus plantarum fermentation broth and nano zinc oxide

[0030] a Culture of bacteria

[0031] Pick a single colony of Bacillus cereus on LB agar medium and place it in 5 mL of LB broth medium, culture it in a 37°C incubator for 12 h, and then inoculate it into 5 mL of LB broth according to the inoculation amount of 1%. In the base, after 5 hours of incubation at 37°C. Take 1 mL of the above bacterial solution and serially dilute the two gradients with 1xPBS water, the final bacterial solution is about 10 8 CFU / mL.

[0032] b The combined effect of Lactobacillus plantarum fermentation broth and nano-zinc oxide on antibacterial properties

[0033] Take 900 μL of the above-mentioned diluted bacterial solution into ...

Embodiment 3

[0040] Preparation of freeze-dried bacterial powder from Lactobacillus plantarum fermentation broth: ① Preparation of bacterial cells: the strains were inoculated in MRS liquid medium with 5% inoculum, anaerobically cultured in an anaerobic incubator at 37°C for 24 hours at a constant temperature, and passed through Microscopic examination confirmed the absence of bacteria. ②Preparation of protective agent: Take out 4.47% trehalose, 13.42% D-sorbitol, and 9.24% skimmed milk powder and dissolve them in 100mL sterile water in advance, then put them in a glass bottle with a stopper, and sterilize at 121°C for 30 min. ③ On the aseptic operating table, mix the fermented Lactobacillus fermentation liquid sample with the sterilized protective agent solution and pour it into a sterile petri dish, and put it in -80°C for pre-freezing for 2 hours. After the sample is completely frozen, quickly Transfer to a freeze dryer for freeze-drying for 24 hours, and the water content of the Lacto...

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Abstract

The invention discloses a bacteriostatic composition of a lactobacillus plantarum fermentation liquid and nano zinc oxide, and belongs to the field of medicine. Combined use of the lactobacillus plantarum fermentation liquid and nano zinc oxide in the bacteriostatic composition plays a combined bacteriostatic effect on inhibition of escherichia coli O157 and bacillus cereus. Compared with the prior art, the bacteriostatic composition of the lactobacillus plantarum fermentation liquid and nano zinc oxide solves the problem that nano zinc oxide is difficult to dissolve in water in a conventional bacteriostatic method, and at the same time, with adopting of the two bacteriostatic substances, the bacteriostatic composition reduces the occurrence probability of microbial acid resistance, reduces toxicity to human bodies, and is safer.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a bacteriostatic mixture. Background technique [0002] Inorganic antibacterial agents and their products have become a research and development hotspot in the field of antibacterial materials due to their unique functions such as safe, reliable, stable and durable antibacterial properties, and have generally attracted people's attention and become the main research object of everyone. Among them, nano-zinc oxide is a white solid with good stability, no discoloration at high temperature, no decomposition, low price, and abundant resources. It has become one of the hot spots in the research of inorganic antibacterial agents. It has a wide range of physiological and biochemical functions in the body, and is also called "life element". As a bacteriostatic agent, nano-zinc oxide has attracted widespread attention in the medical field. [0003] Nano-zinc oxide is made into a bacteriostatic a...

Claims

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Application Information

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IPC IPC(8): A61K33/30A61P31/04A61K35/74
CPCY02A50/30
Inventor 许恒毅况慧娟段宏沈骏邴双飞
Owner 安信生物科技(湖南)有限公司
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