Chromone dimer derivative and its preparation method and use
An application and compound technology, applied in the field of chromone dimer derivatives and their preparation, can solve the problems of unreported antitumor activity and the like
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Embodiment 1
[0098] Embodiment 1: Microbial fermentation culture and preparation of compound 1-3
[0099] 1. Fermentation culture and extraction of fermented products
[0100] 1.1) Production strain
[0101] In this example, the producing bacterium used to ferment and produce compound 1-3 is Penicillium purpurogenum AD-1-2 (Penicillium purpurogenum AD-1-2) of the genus Penicillium. Committee General Microbiology Center, deposit number is CGMCC No.8634.
[0102] 1.2) Fermentation culture
[0103] Get the PDA medium (composition: glucose 2%, agar 2%, NaCl 1.5%, prepare with 20% potato boiled liquid) test tube slant of the PDA culture medium (composition: glucose 2%, agar 2%, NaCl 1.5%) preserved in 4 ℃ of refrigerators, use inoculation Scrape an appropriate amount of bacteria from the loop under sterile conditions, streak and inoculate it on a newly prepared PDA solid medium plate, and activate and culture it in a 28°C incubator for 4 days. From the PDA solid medium plate that has been ...
Embodiment 2
[0140] Embodiment 2: Antitumor activity test of compound 1-3
[0141] 1. Experimental materials
[0142] 1) Preparation of the tested sample solution
[0143] The test samples were pure compounds 1, 2 and 3 isolated and refined in Example 1 above. Docetaxel trihydrate (Beijing Qimiwo Technology Co., Ltd., batch number 20110326) was used as a positive control sample. Precisely weigh an appropriate amount of sample, and use methanol to make a solution of the required concentration for testing the activity.
[0144] 2) Subculture of cell lines and cells
[0145] Human chronic myelogenous leukemia K562 cell line, human acute promyelocytic leukemia HL-60 cell line, human cervical cancer HeLa cell line, and human gastric cancer BGC-823 cell line were used in the activity test. K562, HL-60, HeLa, BGC-823 cells were routinely passaged in RPMI-1640 medium containing 10% fetal bovine serum and 100 μg / ml of penicillin and streptomycin, and the cells were filled with 5% carbon dioxi...
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