Method for fermentation production of micro molecular weight dextran

A dextran and micro-molecular-weight technology, applied in the field of fermentation, can solve problems such as low sucrose conversion efficiency, insufficient material exchange, and large molecular weight of the product, and achieve the effects of favorable fermentation process control, short fermentation cycle, and high fermentation efficiency
CN103993052AInactive Publication Date: 2014-08-20GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
Publication Date
2014-08-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a method for fermentation production of micro molecular weight dextran. According to the method, dextran hydrolase is added to a fermentation system at a bacterial growth logarithmic phase while a sucrose solution is subjected to fed batch so as to effectively improve the conversion efficiency of the sucrose, wherein conversion of 390 g of the sucrose in 24 h in the traditional process is increased to conversion of 750 g of the sucrose in 36 h; and after completing the fed batch, dextran hydrolase is added to the fermentation tank, and the molecular weight (less than 20000 Da) of the micro molecule dextran product can be controlled according to requirements, such that the production of the micro molecular weight dextran through the one-step method can be achieved, acid hydrolysis and alcohol fractional precipitation are not required, and the production process is simplified. According to the present invention, the sucrose conversion efficiency is significantly increased, the effective control effect on the molecular weight of the micro molecule dextran is significant, and the method of the present invention is the effective method for fermentation production of micro molecular weight dextran.
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Description

technical field

[0001] The invention relates to a fermentation method, in particular to a method for producing micromolecular weight dextran by fermentation. Background technique

[0002] Dextran (dextran), also known as glucan, is a high molecular polymer polymerized by several glucose. It is the earliest microbial polysaccharide discovered and the first microbial exopolysaccharide approved by the US FDA for food. It is a microbial polysaccharide that was used as plasma earlier.

[0003] Dextran-70 is currently recognized as one of the excellent plasma substitutes and has been widely used in clinical medicine; Dextran-40 and Dextran-20 have the effect of improving microcirculation and replenishing a certain blood volume. In addition to being used as a plasma substitute, micro-molecular-weight dextran (molecular weight below 20000Da) can also form a series of extremely versatile derivatives, such as iron dextran (molecular weight of dextran is 5000-7500Da), dextran-doxorubi...

Claims

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