Sialoprotein diagnosis model of chronic gastritis diagnosis and construction method thereof

Abstract

The invention discloses a sialoprotein diagnosis model of chronic gastritis diagnosis and a construction method thereof. The construction method of the sialoprotein diagnosis model of chronic gastritis diagnosis comprises the following steps: collecting and treating a saliva sample; treating the saliva sample by adopting a WCX (Weak Cation Exchange) magnetic bead; performing sample application and mass spectrometry; and analyzing according to statistics. The sialoprotein diagnosis model of chronic gastritis diagnosis comprises three sialoprotein peaks with mass-to-charge ratios (m / z) of respectively 5502.36 Da, 1441.75 Da and 3442.47 Da in a human sialoprotein. A classification forecasting model established by three protein peaks with mass-to-charge ratios (m / z) of respectively 5502.36 Da, 1441.75 Da and 3442.47 Da in the human sialoprotein is used for detecting the m / z of the corresponding proteins in the human saliva and then performing analysis by using the model, and can be used for primarily diagnosing the chronic gastritis diagnosis with a recognition rate of 91.67 percent and a predictive ability of 73.33 percent. The sialoprotein diagnosis model disclosed by the invention has an accuracy rate of 90.63 percent (29 / 32), sensitivity of 100 percent (14 / 14) and specificity of 83.33 percent (15 / 18). The construction method disclosed by the invention has the characteristics of simplicity, reasonability, feasibility, and convenience in operation, and can perform massive treatment.

Description

technical field [0001] The invention belongs to the technical field of salivary protein diagnosis, and in particular relates to a salivary protein diagnostic model for diagnosis of chronic gastritis and a construction method thereof. Background technique [0002] Saliva is an indispensable and important body fluid for the human body. Blood components such as various hormones, amino acids, electrolytes, immunoglobulins, and creatinine can enter saliva through the saliva-blood barrier of the capillary wall. As a part of human body fluid, changes in its component content are affected by various pathophysiological changes in the body. Studies have found that saliva components are related to serum, especially studies have confirmed that proteins, which are the main components of saliva, are positively correlated with serum. With the development of science and technology, people's requirements for clinical diagnostic indicators are constantly increasing, not only requiring correc...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the embodiments of the present invention is to provide a salivary protein diagnostic model for the diagnosis of chronic gastritis and its construction method, aiming to solve the problems of lack of diagnostic models and limitations in the existing detection of chronic gastritis

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