Alternaria alternata medium and preparation method thereof
A technology of Alternaria and culture medium, applied in the field of Alternaria culture medium and its preparation, which can solve the problems of low spore production of Alternaria culture medium and difficulty in meeting the needs of industrialization, and achieves a simple preparation method Ease of operation, increased spore yield, and cheap and readily available ingredients
Inactive Publication Date: 2014-09-03
YUNNAN ACAD OF TOBACCO AGRI SCI
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Problems solved by technology
[0003] The object of the present invention is to provide a kind of Alternaria culture medium and preparation method thereof, aim at solving the problem that the existing Alternaria culture medium produces low sporulation, is difficult to meet the needs of industrialization
Method used
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Embodiment 1
[0015] Take 28g of oatmeal, add 500ml of water, heat it on a boiling water bath for 15min, filter it with three layers of gauze, take the filtrate, add 65g of dried tobacco leaves, 12g of soybean powder, 16g of agar, add distilled water to make up 1000ml, subpackage and sterilize to obtain Alternaria Medium 1.
Embodiment 2
[0017] Take 32g of oatmeal, add 500ml of water, heat in a boiling water bath for 15min, filter through three layers of gauze, take the filtrate, add 55g of dried tobacco leaves, 8g of soybean powder, 20g of agar, add distilled water to make up 1000ml, subpackage and sterilize to obtain Alternaria Medium 2.
Embodiment 3
[0019] Take 30g of oatmeal, add 500ml of water, heat on a boiling water bath for 15min, filter through three layers of gauze, take the filtrate, add 60g of dried tobacco leaves, 10g of soybean powder, 18g of agar, add distilled water to make up 1000ml, subpackage and sterilize to obtain Alternaria Medium 3.
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The invention provided an alternaria alternata medium and a preparation method thereof. The preparation method comprises: taking 28-32 g of oatmeal, adding 500 mL of water, heating in boiling-water bath for 15 min, filtering by using three layers of gauze, taking the filtrate, adding 55-65 g of dry tobacco leaves, 8-12 g of soybean powder and 16-20 g of agar, and adding distilled water to have a total volume of 1000 mL, and performing split charging and sterilization to obtain the alternaria alternata medium. The alternaria alternata medium is cheap and easily-available in compositions, the preparation method is simple and easy to operate, and after the alternaria alternata medium is applied to inoculation of alternaria alternata spawns, the spore output is greatly improved.
Description
technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to an Alternaria culture medium and a preparation method thereof. Background technique [0002] The existing Alternaria culture medium mainly includes CA medium and PDA medium, wherein, the CA medium is prepared from oatmeal, and the PAD medium is made from potato, glucose and agar. Although CA medium and PDA medium can satisfy the basic growth of Alternaria, their sporulation yield is low, generally around 100-200 per dish, which is difficult to meet the needs of large-scale quantitative inoculation. Contents of the invention [0003] The object of the present invention is to provide an Alternaria culture medium and a preparation method thereof, aiming at solving the problem that the existing Alternaria culture medium has a low sporulation yield and is difficult to meet the needs of industrialization. [0004] The present invention is achieved in that a...
Claims
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IPC IPC(8): C12N1/14C12R1/645
Inventor 于海芹肖炳光黄昌军
Owner YUNNAN ACAD OF TOBACCO AGRI SCI