Breast carcinoma gene BRCA1/BRCA2 mutation detection kit and preparation method thereof

A susceptibility gene and mutation detection technology, applied in the field of medical molecular biology, to achieve the effect of ensuring accuracy, meeting the needs of scientific research and clinical testing, and simple operation

Inactive Publication Date: 2014-09-03
杭州美中疾病基因研究院有限公司
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process will take a lot of time for experiment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Breast carcinoma gene BRCA1/BRCA2 mutation detection kit and preparation method thereof
  • Breast carcinoma gene BRCA1/BRCA2 mutation detection kit and preparation method thereof
  • Breast carcinoma gene BRCA1/BRCA2 mutation detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0043] In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with examples. It should be understood that the examples described here are only used to explain the present invention, but not to limit the present invention.

[0044] In the present invention, three site primers that have been designed and verified are added to the common PCR reaction solution system to prepare a premix solution, and each tube is marked. Then use the same template to test the performance of the premix at different time points. Specific steps are as follows:

[0045] (1) DNA extraction from peripheral blood of volunteers with a family history of breast cancer: (using LIFE Science Whole Biood DNA Mini KIT)

[0046] 1. Take a clean 1.5ml EP tube, add 500μl of Buffer AC1, then add 200μl of blood sample, mix and shake for 30s

[0047] 2. Add 100μl Buffer AC2, mix quickly and shake f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a breast carcinoma gene BRCA1/BRCA2 mutation detection kit. The kit includes three primer pairs for specific amplification of BRCA1 / BRCA2 gene targeting sequences; a primer pair1 is F:GGTTGGCAGCAATATGTG; and R:AGAGGCAGAGTGGATGGA; a primer pair2 is: F:CTCTTATCCTGATGGGTTGT; and R:TCTCATCTGCTTAAAGTCCC; a primer pair 3 is: F:GGCAGGTTGTTACGAGGCAT; and R: GTGAGCTGGTCTGAATGTTCG. The produced fragments of the three primer pairs are more appropriate in size, the accuracy of direct sequencing of a PCR amplified product can be ensured. The kit has the advantages of simple operation, a site sequence can be amplified only by adding a template on a PCR instrument to obtain a specific product, and the kit is convenient and quick.

Description

technical field [0001] The invention belongs to the field of medical molecular biology and relates to a kit, in particular to a kit for detecting hot spot mutations of breast cancer susceptibility genes BRCA1 / BRCA2. Background technique [0002] The close relationship between BRCA1, BRCA2 susceptibility genes and breast cancer has received more and more attention, and the research is also deepening. The high correlation between BRCA1 and BRCA2 and familial hereditary breast cancer is beyond doubt. The detection of BRCA1 can reflect the occurrence and development of breast cancer, and can also screen out high-risk groups of breast cancer, ovarian cancer and other related malignant tumors, which is conducive to the early diagnosis and treatment of such diseases. Although the specific mechanism and pathway of BRCA2's tumor suppressor effect are not yet clear, breast cancer and several other malignant tumors, such as prostate cancer and ovarian cancer, can be detected early by ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2531/113
Inventor 陈伟
Owner 杭州美中疾病基因研究院有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap