Breeding method for high-protein polyploid paddy rice line
A rice strain, high-protein technology, applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of low seed setting rate of polyploid rice, restricting the application of high-protein strains, etc., to improve the application value and scope , Improving dietary structure and nutrition, and increasing protein content
Active Publication Date: 2014-09-10
HUBEI UNIV +1
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Problems solved by technology
Although polyploidization can significantly increase the protein content of rice, the low seed setting rate of polyploid rice has long restricted the application of high protein strains
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[0026] Below to Example - The breeding of high protein polyploid rice - HG383 further illustrates the present invention.
[0027] a. Using the PMeS line (2x) as the basis parent to cross with an indica or japonica rice line
[0028] Using the PMeS line HN2026-2x as the basic parent to cross with the indica rice dts-70-2x;
[0029] b. Select another japonica or indica variety for compound crossing
[0030] Composite cross of F1 with dts76-2x (japonica rice) obtained from the cross of a;
[0031] c. The compound hybrids are doubled through tissue culture to form polyploid rice plants
[0032] When the hybrid hybrids obtained in b are planted until the young spikes are differentiated, the young spikes in the second branch—the meiotic stage are taken for tissue culture, and the vigorously growing callus formed is placed in a mixture containing 450mg / L~760 mg / L colchicine in N6 liquid medium, cultured on a shaking table for 50hr~60hr, after 7 days of recovery culture, then tr...
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The invention provides a breeding method for a high-protein polyploid paddy rice line. The polyploid paddy rice line with high protein content is selected by subjecting a PMeS line and appropriate indica type rice or japonica rice lines to hybridization and composite cross, doubling a hybrid and carrying out thremmatological characteristic and protein content determination. The method comprises the following steps: a, with the PMeS line (2x) as a basic parent and subjecting the PMeS line and one indica type rice or japonica rice line to hybridization; b, carrying out composite cross with another indica type rice or japonica rice line; c, subjecting the hybrid obtained after composite cross to tissue culture doubling so as to form a polyploid paddy rice strain; d, carrying out thremmatological characteristic selection on the formed polyploid paddy rice strain; e, carrying out preliminary assessment on the protein content of the seed of the formed polyploid paddy rice strain (wherein the protein content is more than 10%); f, carrying out comprehensive evaluation on later generations before selection; g, carrying out certainty detection on an inbred stable line; and h, breeding the selected line into the stable polyploid paddy rice line with a high protein content (10 to 13%).
Description
technical field [0001] The invention relates to a method for breeding high-protein polyploid rice strains, belonging to the technical field of rice breeding in modern agriculture. Background technique [0002] Rice is the most important food source for humans after wheat. However, compared with wheat, rice nutrition has the characteristics of easier absorption and utilization. However, the protein content of rice is low, generally only 7% to 9% in popularized varieties. Although the protein content of rice can be significantly increased through polyploidization, the low seed-setting rate of polyploid rice has long restricted the application of high-protein strains. For this reason, in order to breed new polyploid rice lines with high protein content, it is necessary to simultaneously obtain the characteristics of high protein content and high seed setting rate. The present invention is to make full use of the characteristics of high fruiting characteristics and polyploidi...
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IPC IPC(8): A01H1/04
Inventor 蔡得田宋兆建王维何玉池张献华刘育华
Owner HUBEI UNIV