Preparation method and application of nerve growth factor controlled-release nano-carrier

A nerve growth factor and nanocarrier technology, used in the preparation of drugs for diabetes combined with coronary heart disease, a nerve growth factor sustained release system (nanocarrier) and its preparation, and the field of preparation of medicines for improving the function of diabetic cardiac autonomic and sensory nerves , can solve the problem of unoptimistic treatment effect, and achieve the effect of increasing stability and action time, non-toxic encapsulation and film formation, and good biocompatibility

Inactive Publication Date: 2014-09-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the existing treatment methods start from the perspective of lowerin

Method used

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  • Preparation method and application of nerve growth factor controlled-release nano-carrier
  • Preparation method and application of nerve growth factor controlled-release nano-carrier
  • Preparation method and application of nerve growth factor controlled-release nano-carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: PLGA-NGF production

[0026] Add 500 μg NGF to 250 μl borate buffer solution (0.05M), weigh 0.5 mg FITC and add it to 250 μl protein system, mix well until FITC is completely dissolved. React for 1 hour at room temperature in the dark, then apply purification column for purification, centrifuge at 1000g for 30-45 seconds, collect the purified protein, and refrigerate at 4°C in the dark. Inner water phase (W 1 ), 200 μl of FITC-labeled NGF was added with 5% PEG; oil phase (O), 25 mg of PLGA was dissolved in 2 ml of a mixed solution of dichloromethane and acetone with a volume ratio of 3:1, and the protein solution was added to the PLGA solution, Prepare water-in-oil emulsion (W 1 / O). Will (W 1 / 0) Emulsion is added in the aqueous solution that contains 0.7%PVA in 10ml, ultrasonic 20min in 55W ice water, obtains water-in-oil-in-water type double emulsion (W 1 / O / W 2 ). Pour the uniform double emulsion into a centrifuge tube, and evaporate in vacuum at...

Embodiment 2

[0027] Example 2: Observation of surface morphology of PLGA-NGF

[0028] Weigh 1 mg of PLGA-NGF nanoparticle freeze-dried powder, add it to 1 ml of distilled water and stir to form a white emulsion, absorb a small amount of emulsion and drop it on the copper grid, then add 2% phosphotungstic acid solution for negative staining, and let it dry naturally. The surface morphology of the particles was then observed under a transmission electron microscope (TEM). see figure 1 .

Embodiment 3

[0029] Embodiment three: the mensuration of nanoparticle average particle size and particle size distribution

[0030] Weigh a little PLGA-NGF nanoparticle freeze-dried powder, dilute it with distilled water to a slightly turbid concentration, and measure the average particle size and For particle size distribution, 14 cycles were taken, three independent samples were taken, and each sample was measured three times.

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Abstract

The invention provides a preparation method and application of a nerve growth factor controlled-release nano-carrier. A nerve growth factor and polylactic acid-hydroxyacetic acid copolymer are prepared into spherical nano-particles by an emulsion evaporation solvent method. A nerve growth factor nano controlled-release system packaged with the polylactic acid-hydroxyacetic acid copolymer is of a core-shell structure, wherein the core is the nerve growth factor, and the shell is uniform and compact polylactic acid-hydroxyacetic acid copolymer. According to the invention, proble that the existing nerve growth factor has a short body distribution half-life period and biological activity half-life period, can be metabolized quickly and degraded by enzyme easily and extremely unstable in an aqueous solution is solved. The method disclosed by the invention is simple to operat, and controllable in process. Moreover, secondary development on range of application of the nerve growth factor is carried out, the medicinal range of the nerve growth factor is expanded, and the never growth factor can be applied to preparation of a drug for treating the coronary heart disease with diabetes on the basis of the nerve protection effect of the nerve growth factor nano controlled-release system.

Description

technical field [0001] The invention belongs to the preparation of medicines, and specifically relates to a nerve growth factor sustained-release system (nano-carrier) wrapped in polylactic acid-glycolic acid copolymer and its preparation method, as well as its application in the preparation of drugs for diabetes combined with coronary heart disease, which is suitable for the preparation of drugs for improving diabetic heart disease. Drugs for autonomic and sensory functions. Background technique [0002] Nerve growth factor (NGF) is the earliest neurotrophic factor discovered and the most thoroughly studied at present. It is a nerve cell growth regulator with dual biological functions of neuron nutrition and neurite growth. Development, differentiation, growth, regeneration, and expression of functional properties are all important regulators. After nerve injury, NGF receptors increase, reflecting the demand for NGF in the process of injury repair. The level of NGF in the...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61K47/34A61K9/51A61P3/10A61P9/10
Inventor 郑良荣赵文婷张媛媛孙泽玮韩飞
Owner ZHEJIANG UNIV
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