Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of monoclonal antibody of ultraviolet excision repair protein and its application

A monoclonal antibody, repair protein technology, applied in anti-animal/human immunoglobulin, biochemical equipment and methods, material testing products, etc., can solve problems such as differences

Active Publication Date: 2016-06-29
CHENGDU ZEN BIOSCI +2
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In patients with low expression of hHR23b, the response rate was only 41.7%, with a significant difference

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of monoclonal antibody of ultraviolet excision repair protein and its application
  • A kind of monoclonal antibody of ultraviolet excision repair protein and its application
  • A kind of monoclonal antibody of ultraviolet excision repair protein and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Obtaining the hybridoma cell line 5H1-A10-A7 and the monoclonal antibody it produces.

[0021] (1), animal immunity

[0022] 6-8 week old female BALB / c mice were immunized with recombinant human hHR23b protein as antigen, and three immunization injections were performed according to the pre-established immunization method.

[0023] The sequence structure of the antigenic protein is:

[0024] MQVTLKTLQQQTFKIDIDPEETVKALKEKIESEKGKDAFPVAGQKLIYAGKILNDDTALKEYKIDEKNFVVVMVTKPKAVSTPAPATTQQSAPASTTAVTSSTTTTVAQAPTPVPALAPTSTPASITPASATASSEPAPASAAKQEKPAEKPAETPVATSPTATDSTSGDSSRSNLFEDATSALVTGQSYENMVTEIMSMGYEREQVIAALRASFNNPDRAVEYLLMGIPGDRESQAVVDPPQAASTGAPQSSAVAAAAATTTATTTTTSSGGHPLEFLRNQPQFQQMRQIIQQNPSLLPALLQQIGRENPQLLQQISQHQEHFIQMLNEPVQEAGGQGGGGGGGSGGIAEAGSGHMNYIQVTPQEKEAIERLKALGFPEGLVIQAYFACEKNENLAANFLLQQNFDED

[0025] For the first immunization, 0.2ml emulsion (100ul purified recombinant protein hHR23b (diluted with 1×PBS) + 100ul complete Freund's adjuvant, containing 100u...

Embodiment 2

[0037] Example 2: Identification and Application of Monoclonal Antibody

[0038] 1. Western blot detection and identification of mouse anti-hHR23b monoclonal antibody (anti-hHR23b)

[0039] Extract the cell lysates of various tumor cell lines, adjust the total protein concentration to 2mg / ml, load the samples into 12% SDS-PAGE gel holes, 20ug / well, and transfer the proteins in the gel to PVDF membrane after electrophoresis , after blocking with 5% skimmed milk powder, immunostaining was carried out with the anti-hHR23b antibody prepared in Example 1: add the anti-hHR23b prepared and purified in step (3) of Example 1, the working concentration was 1:1000, and incubated overnight at 4 degrees , and then add HRP-labeled goat anti-mouse antibody at a working concentration of 1:20000, and react at 37°C for 1 hr, and a 55Kd band appears in each lysate (see figure 2 ), consistent with literature reports, proving that this antibody is a specific antibody against hHR23b; the antibody...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a monoclonal antibody of UV excision repair protein and its application, in particular, to a monoclonal antibody of UV excision repair protein (hHR23b), a hybridoma cell line secreting the monoclonal antibody, and the expression of the antibody in Application in Predicting Clinical Treatment Effects of HDAC Inhibitors.

Description

technical field [0001] The invention relates to a monoclonal antibody of ultraviolet excision repair protein (hHR23b), a hybridoma cell line secreting the monoclonal antibody and the application of the antibody. Background technique [0002] Histone deacetylase (HDAC, Histonedeacetylase) is a relatively new and very potential tumor therapeutic target. In 2006, the FDA approved Vorinostat, the first new HDAC inhibitor drug, to obtain the "breakthrough drug" qualification for the treatment of cutaneous T-cell lymphoma (CTCL) that is exacerbated, persistent and relapsed or ineffective after treatment with two systemic drugs. , a type of non-Hodgkin's lymphoma). The development of new drugs targeting HDAC inhibitors has entered a new level. In 2013, the FDA granted Entinostat "Breakthrough Therapy" certification, which was combined with exemestane for the treatment of postmenopausal breast cancer patients whose disease worsened after treatment with non-steroidal aromatase inhi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/18C12N5/20G01N33/68G01N33/577
Inventor 邱俊康聂子梅
Owner CHENGDU ZEN BIOSCI