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Fomes fomentarius compound extract and preparation method and application thereof

An extract and technology of wood hoof, applied in the field of wood hoof compound extract and its preparation, can solve the problems of pine needle toxicity, etc., and achieve the effect of improving immune function, thymus index and peripheral blood TNF-a content

Inactive Publication Date: 2014-09-24
NAVY MEDICINE RES INST OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pine needles are toxic. In order to achieve the purpose of strengthening the body and eliminating evils in the theory of traditional Chinese medicine, pine needles must be combined with other traditional Chinese medicines to relieve toxicity and be used safely.

Method used

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  • Fomes fomentarius compound extract and preparation method and application thereof
  • Fomes fomentarius compound extract and preparation method and application thereof
  • Fomes fomentarius compound extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 wood hoof compound extract

[0029] 60g of Phyllostachys chinensis, 30g of Phylophora pine needles and 15g of Chizhi, crushed, added water, heated and refluxed at 100°C for 3 times, each time for 2h, the ratio of solid to liquid was 1:20, 1:15 and 1:10 respectively, filtered , combine the filtrates, let stand at 4°C for 24h, centrifuge at 9000r / min for 30min, concentrate the upper solution under reduced pressure to 20 times the volume, add 95% ethanol to the concentrated solution to make the final ethanol concentration 80%, let stand at 4°C for 24h, 9000r / min After centrifuging for 30 min, the supernatant was concentrated under reduced pressure to 20 times the volume, and the concentrated solution was vacuum-dried to obtain the final product.

Embodiment 2

[0030] The preparation of embodiment 2 wood hoof compound extract

[0031] Grind 70g of P. chinensis, 20g of P. pine and 15g of red lucidum, add water, heat and reflux at 100°C for 3 times, each time for 2h, the ratio of solid to liquid is 1:20, 1:15 and 1:10 respectively, and filter , combine the filtrates, let stand at 4°C for 24h, centrifuge at 9000r / min for 30min, concentrate the supernatant under reduced pressure to 20 times the volume, add 95% ethanol to the concentrate to make the final ethanol concentration 80%, let stand at 4°C for 24h, 9000r / min After centrifuging for 30 min, the supernatant was concentrated under reduced pressure to 20 times the volume, and the concentrated solution was vacuum-dried to obtain the final product.

Embodiment 3

[0032] Example 3 Wood Hoof Compound Extract Composition Analysis

[0033] (1) Component analysis

[0034] Dissolve the compound extract of the wood hoof in 80% ethanol solution to obtain the test solution, and then use the system pretest method to identify it. ① Test tube pre-test method Add relevant reagents into the test tube containing the test solution, and judge the existence of a certain chemical component according to the reaction phenomena such as color, precipitation, and fluorescence. ②Circular filter paper layer method Use a capillary spotting tube to absorb the test solution and spot the sample on the circular filter paper. After drying, spread the ethanol from the center and spray with different color developers. According to the color, spots or fluorescence produced, etc. Phenomenon to judge the presence of a chemical component. The results are shown in Table 1. It can be known that it contains organic acids, sugars, polysaccharides and glycosides, saponins, la...

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PUM

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Abstract

The invention relates to the technical field of biological medicine. In particular, the invention relates to a fomes fomentarius compound extract, a preparation method and an application thereof. The fomes fomentarius compound extract is prepared from the following components according to a weight ratio of 1-10 parts of fomes fomentarius:1-9 parts of phellinus pini:1 part of ganoderma lucidum; the extraction method of the compound extract comprises: preparing a fomes fomentarius compound, mixing, crushing, adding pure water, performing water extraction and alcohol precipitation to obtain a supernatant, performing reduced pressure concentration, and performing vacuum drying of the concentrate to obtain the product. The content of triterpene and triterpene saponin in the fomes fomentarius compound extract of the invention is not less than 5.5%. The extract not only has significant in-vivo and in-vitro antineoplastic efficacy, but also has no obvious acute toxicity; the maximum tolerance dose for mice is 0.2 g (equivalent to 4.58 g of crude drugs), which is 327 times of human oral dosage. The extract can be prepared into compound preparation when mixed with pharmaceutically acceptable carriers, and the compound preparation can be oral liquid, injection, suspension, pills, tablets, capsules, powder, granules, or drop pills.

Description

1 technical field [0001] The invention relates to the technical field of biomedicine. Specifically, the present invention relates to a compound extract of wood hoof and its preparation method. The compound extract is mainly used for inhibiting the growth of tumor cells in vitro and in vivo. 2 background technology [0002] In recent years, the incidence of cancer in my country has been in a period of rapid rise, with about 2.6 million cancer cases and 1.8 million deaths each year. It has become the number one cause of death for urban and rural residents in China. Due to the inherent defects in tumor surgical resection, chemotherapy and radiotherapy, the anti-tumor research of natural medicine or traditional Chinese medicine has been paid more and more attention. Medicinal fungi are an important part of natural medicine, and anti-tumor effect is one of the important functions of medicinal fungi. [0003] Among them, Fomes fomentarius (L.: Fr) Kick, also known as chaga, is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61P35/00A61P37/04
Inventor 刘琼沈先荣何颖蒋定文刘玉明侯登勇陈伟王庆蓉钱甜甜莫琳芳
Owner NAVY MEDICINE RES INST OF PLA
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