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A method for producing forage plant protein by enzymolyzing plant protein through co-fermentation with enzyme bacteria

A technology for enzymatic hydrolysis of plant protein and plant protein, applied in animal feed, animal feed, application, etc., to achieve the effects of good compatibility, good enzymatic hydrolysis, and easy control of product process

Active Publication Date: 2016-07-06
湖北邦之德牧业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] The object of the present invention is to provide a method for producing forage plant protein by enzymolyzing plant protein through enzyme-bacteria synergistic fermentation, to solve the problems in the industrialization of fermented protein, including bacterial strain combination, enzyme-bacteria synergistic process, uniform mixing of material and water, fermentation mode and control. , drying and energy saving, material crushing and recycling, etc., strengthen the controllability of the fermented vegetable protein process, and improve product quality and stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: It is a basic embodiment of the present invention. A method for producing forage plant protein by co-fermentation with enzymes and bacteria. The enzyme bacteria used for fermentation and enzymolysis include compound enzyme preparations and microbial strains; the plant protein materials used for fermentation and enzymolysis are selected from two or more Two or more plant seeds are compressed or fermented to extract the remaining meal or grains; the method of producing feed protein includes: ingredients, inoculation and mixing, fermentation and enzymatic hydrolysis, drying, cooling, crushing, product testing, and the final feed The small peptide content of vegetable protein products is 10-40% of the protein contained.

Embodiment 2

[0043] Example 2: It is a further example of Example 1. The present invention provides a method for producing plant protein for feed by co-fermenting and enzymatically hydrolyzing plant protein with enzymes and bacteria. The remaining meal or grains after the plant seeds are compressed or fermented and processed include soybean meal, cottonseed meal, rapeseed meal, and distillers grains .

Embodiment 3

[0044] Example 3: It is a further example of Example 1. The method of the present invention uses enzyme bacteria to synergistically ferment and enzymolyze vegetable protein to produce feed vegetable protein, the steps are:

[0045] A. Ingredients: The vegetable protein raw materials used for fermentation and enzymatic hydrolysis are selected from soybean meal, cottonseed meal, rapeseed meal, distillers grains, two or more of them in combination; B. Inoculation and mixing: The enzyme bacteria used for fermentation enzymolysis are compound enzyme preparations, Bacillus subtilis BZD-01, and Saccharomyces cerevisiae BZD-02. Mix the three enzyme bacteria with the plant protein material of step A to form a mixture; C. Fermentation enzymolysis: control the temperature and oxygen of the mixture material in step B, and use the secondary temperature to control the fermentation enzymolysis to obtain the fermentation enzymolysis product; D. Drying: fluidized bed drying of the fermented enz...

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PUM

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Abstract

The invention relates to a method for producing a forage plant protein by carrying out enzyme synergy fermentation and enzymolysis on a plant protein. The method is characterized in that enzyme for the fermentation and the enzymolysis comprises a complex enzyme preparation and microbial strains; a plant protein raw material for the fermentation and the enzymolysis is extracted from residual processed meal or grains after two or more kinds of plant seeds are compressed or fermented. The method comprises the steps of preparing the materials, inoculating and mixing the materials, carrying out the fermentation, carrying out the enzymolysis, drying, cooling, crushing and carrying out product detection, so as to finally obtain a forage plant protein product, wherein the content of small peptide of the forage plant protein product is 10-40% of that of the proteins of the forage plant protein product. The method has the advantages that the key technical problems of high labor intensity, difficulty in control, instable quality, low production efficiency, high energy consumption and the like in the solid state fermentation process of the plant proteins can be solved, the industrialization of a fermented plant protein product can be effectively promoted, and the economic benefit can be increased.

Description

Technical field [0001] The invention relates to a method for producing plant protein for feed by co-fermenting and enzymatically hydrolyzing plant protein with enzyme bacteria, and belongs to the field of bioengineering. Specifically, it is an industrialized engineering technology to transform plant protein by using a composite enzyme preparation, Bacillus subtilis BZD-01, and Saccharomyces cerevisiae BZD-02 synergistic fermentation and enzymatic hydrolysis technology to produce high-quality feed protein. Background technique [0002] 1. The demand for high-quality plant protein in feed breeding industry: [0003] Feed breeding industry is one of the pillar industries in many countries, therefore, the total demand for feed is huge; [0004] The development of the feed industry must rely on high-quality raw materials, especially protein raw materials. But there are the following problems: [0005] (1) Shortage of animal protein resources and safety issues; [0006] (2) Soybean meal ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/00A23K10/30A23K20/00A23K20/189A23K10/12A23K10/14A23K10/18A23K10/37A23K10/38A23K20/147
CPCY02P60/87
Inventor 吴晓峰葛向阳张涛胡敏苏树和廖媛媛李苏
Owner 湖北邦之德牧业科技有限公司