Device for early detection of micro-organisms

A technology for early detection and microorganisms, applied in the determination/inspection of microorganisms, biological tests, measuring devices, etc., which can solve the problems of long time, pollution, and high cost

Active Publication Date: 2014-10-01
BIOMERIEUX SA
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  • Claims
  • Application Information

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Problems solved by technology

[0006] The disadvantage of this method is that relatively long incubation periods are required in order for the colonies formed on the agar to be detectable with the naked eye
[0008] The disadvantage of these methods is that several kinds of DNA strands are required, that is, several kinds of contaminating microorganisms, ge

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  • Device for early detection of micro-organisms
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  • Device for early detection of micro-organisms

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Embodiment Construction

[0042] The device 1 according to the invention enables a particularly early detection of contaminated microorganisms, for example bacteria, yeasts, mycoplasmas, molds, which are ultimately present in the sample to be analyzed. In fact, this device enables the detection of colonies even though they remain invisible to the naked eye, and, using the light emitted naturally from said colonies, does not require the addition of reagents nor the measurement of natural or autofluorescence. This device is attached figure 2 And the device shown in accompanying drawing 3.

[0043]As for the samples that have to be analyzed, they can start from industries in various fields, for example, pharmaceutical, agricultural products, biotechnology or even the cosmetics industry. In fact, in this type of industry, the rapid detection of the presence of final contaminants is of particular interest, even necessary. In fact, the products manufactured and / or converted in this type of industry are pa...

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Abstract

The present invention relates to a device (1) permitting an early detection, without measurement of the fluorescence, of colonies resulting from the multiplication of micro-organisms present in a sample to be tested, said device (1) comprising: - a substantially flat and horizontal detection surface (5), on which at least one support (3) for growing the micro-organisms in the form of colonies is arranged immobile, this support (3) being of the type membrane or agar medium, - a detection system (7), such as a linear scanner, mounted movable and flat for scanning the whole or part of said surface (5), including at least one CCD sensor (8), which an optical system comprised of at least one lighting (9) and at least one optical means, such as a lens (10), is associated with, the CCD sensor (8) of the detection system (7) has a resolution higher than or equal to 2400 dpi, and the detection system (7) permits the detection by imaging of colonies having a diameter smaller than 50 [mu]m through a useful magnification higher than or equal to 60.

Description

technical field [0001] The invention relates to the field of detection of growth stage microorganisms. [0002] The present invention will mainly be applied in the field of industrial microbiology, for example, pharmaceutical, biotechnology or agro-industries. [0003] In particular, the present invention relates to a device enabling early detection of colony formation on the surface of membranes, solid or semi-solid growth media based on the presence of microorganisms in a sample. Background technique [0004] Currently, there are many techniques implemented to detect contaminants, such as bacteria, in the samples to be tested. [0005] The most common and oldest method involves deposition on the surface of an agar medium, where the medium is more or less selective for one or a few types of microorganisms. The medium is incubated at a suitable temperature for the growth of the microorganisms sought for a period which may usually extend to several days. [0006] The disad...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCC12Q1/04C12M41/14C12M41/36G01N21/00
Inventor 约瑟夫·皮尔奎
Owner BIOMERIEUX SA
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