Method for preparing laccase-containing fermentation broth by pycnoporus sanguineus

Abstract

The invention belongs to the field of microbial fermentation, and particularly relates to a method for preparing laccase-containing fermentation broth by pycnoporus sanguineus. The method comprises the steps as follows: under aerobic and dark conditions, pycnoporus sanguineus CGMCC No. 2932 is cultivated in a sterile fermentation medium containing a carbon source, a nitrogen source and inorganic components, the fermentation medium comprises cornmeal, bran powder, ammonium tartrate, monopotassium phosphate, magnesium sulfate heptahydrate, sodium succinate, copper sulfate pentahydrate, potassium chloride hexahydrate, zinc vitriol, a surfactant, an antifoaming agent and water, the ventilation quantity is adjusted in a fermentation process, and nutrients are fed-batched. The method for preparing the laccase-containing fermentation broth by pycnoporus sanguineus solves the problems of poor reproducibility, low enzyme activity and the like in the prior art, and has the advantages of good reproducibility, high activity of produced laccase, purer produced enzyme system, good enzyme temperature stability, high pH adaptability and the like; and the laccase can be widely applied to the fields of dye degradation, biobleaching, wood gluing, ecological restoration and the like.

Description

technical field [0001] The invention belongs to microbial fermentation, in particular to a method for preparing a laccase-containing fermented liquid by using Microporosa haemophilus. Background technique [0002] Laccase is a copper-containing polyphenol oxidase that catalyzes the redox reaction of phenols. Laccase has special properties of substrate non-specificity, and has broad-spectrum degradation ability to many high-molecular organic substances with different structures and difficult to degrade. fields. As a biocatalyst, laccase has the advantages of high efficiency and low energy consumption compared with physical and chemical methods. [0003] At present, the main sources of laccase in the domestic market are Sigma Company of the United States and Novozymes Company of Denmark. Due to its high price and other reasons, its application in related domestic fields is seriously restricted. The production of laccase in natural strains is generally low, which is far from...

AI Technical Summary

Problems solved by technology

[0003] At present, the main sources of laccase in the domestic market are Sigma Company of the United States and Novozymes of Denmark. Due to its high price and other reasons, its application in related domestic fields is seriously restricted.

The production of laccase in natural strains is generally low, which is far from meeting the needs of production and research. Whether a large amount of laccase preparations with reasonable prices can be obtained for production and research has become one of the key factors restricting the application of laccase

[0004] The Institute of Microbiology of the Chinese Academy of Sciences disclosed "a laccase and its preparation method and special production strain" in the invention patent with the patent number 200910083514.7. In this patent, the laccase prepared by fermentation of Pycnoporus sanguineus CGMCCNo.2932 was used The enzyme activity can reach 300u / ml, but the published formula and process are shake flask fermentation parameters, and the reproducibility of the enlarged culture in the fermenter is poor. Therefore, the research is suitable for large-scale industrial production, and the enzyme activity is high The production process of laccase is the main technical problem at present

Images