Apparatus for rapidly detecting soybean proteins in food, and making method thereof

Abstract

The invention discloses an apparatus for rapidly, conveniently, efficiently and quantitatively detecting soybean proteins in a food, and a making method thereof, and belongs to a fluorescence microsphere labeled detection technology. The apparatus comprises a special property labeled biological macromolecule with biological specificity and a polymeric material film with adsorption mobility as carriers, and detection is carried out in the portable and small square apparatus. The special property of a tag is used to quantitatively detect a trace amount of soybean proteins contained in the matrix of the food, and the detection limit reaches 0.1ng / mL. The special tag overcomes the problems of instability and low detection value of common tags. The apparatus is mainly used in the quantitative detection of residual soybean allergens in food safety.

Description

technical field [0001] The invention belongs to the field of detection of soybean protein in food in food safety, and in particular relates to a device for qualitatively and quantitatively rapidly detecting fluorescent microspheres of soybean protein in food and a preparation method thereof. Background technique [0002] In recent years, with the improvement of people's living standards, food safety issues have become more and more prominent, including food allergies. Food allergy refers to an allergic reaction of the body to a certain substance or component in food. It is a hypersensitivity reaction mediated by IgE and non-IgE, which can cause reactions in the skin, gastrointestinal tract, and respiratory system. In severe cases, it may even lead to systemic allergic reactions or shock. [0003] Soybean products are rich in protein and balanced amino acid components. They are high-quality plant protein sources for humans and livestock, but they are also one of the eight ma...

AI Technical Summary

Problems solved by technology

[0007] (1) The colloidal gold labeling process is an electrostatic adsorption process, which is a physical adsorption, so it has poor stability in the liquid phase.

[0008] Often cause the labeled protein molecules to fall off again

[0009] (2) The result is judged by displaying a single purple-red strip, which is single in color, and it is difficult to achieve multiple detection and linkage.

[0012] The bands have little contrast to the background, limiting detection sensitivity

[0013] (4) The matrix effect of different materials is obvious, and the background interference is very large

[0014] (5) Detection sensitivity is low

[0015] (6) Unable to achieve accurate quantitative detection

[0019] However, the quantitative detection of soybean protein by conventional fluorescent microsphere immunochromatography method is still difficult to meet the requirements of sensitivity and detection stability.

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