Apparatus for rapidly detecting soybean proteins in food, and making method thereof

A technology of soybean protein and food, which is applied in the direction of measuring devices, material inspection products, biological testing, etc., can solve the problems of difficult to achieve multi-detection and connection, large background interference, low detection sensitivity, etc., achieve long service life, easy to manufacture, The effect of sensitive quantitative detection

Inactive Publication Date: 2014-10-15
NANCHANG UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) The colloidal gold labeling process is an electrostatic adsorption process, which is a physical adsorption, so it has poor stability in the liquid phase.
[0008] Often cause the labeled protein molecules to fall off again
[0009] (2) The result is judged by displaying a single purple-red strip, which is single in color, and it is difficult to achieve multiple detection and linkage.
[0012] The bands have little contrast to the background, l

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Apparatus for rapidly detecting soybean proteins in food, and making method thereof
  • Apparatus for rapidly detecting soybean proteins in food, and making method thereof
  • Apparatus for rapidly detecting soybean proteins in food, and making method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Assembly of fluorescent labeling device;

[0055] 1. Preparation of NC membrane:

[0056] Preparation of detection area and quality control area: Soybean protein polyclonal antibody and anti-rabbit antibody coated on nitrocellulose membrane: use 0.1 M pH 7.2 PBS (phosphate buffer saline, which contains 5% sucrose and 0.05% Tween -20 (Tween-20)) to adjust the concentration of soybean protein polyclonal antibody to 0.5 mg / mL, the resulting solution was sprayed on the membrane as the detection area, and 0.01 M PBS pH 7.2 (which contained 5 % sucrose and 0.05 % spit Tween-20 (Tween-20)) adjusted the concentration of anti-rabbit antibody to 0.5 mg / mL, and the resulting solution was sprayed on the membrane as a quality control area. mm, the quality control area was 2 mm away from one end of the nitrocellulose membrane, dried at 37°C overnight, and then stored in a dry environment at room temperature for later use.

[0057] 2. Preparation of fluorescent microsphere pad: ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses an apparatus for rapidly, conveniently, efficiently and quantitatively detecting soybean proteins in a food, and a making method thereof, and belongs to a fluorescence microsphere labeled detection technology. The apparatus comprises a special property labeled biological macromolecule with biological specificity and a polymeric material film with adsorption mobility as carriers, and detection is carried out in the portable and small square apparatus. The special property of a tag is used to quantitatively detect a trace amount of soybean proteins contained in the matrix of the food, and the detection limit reaches 0.1ng/mL. The special tag overcomes the problems of instability and low detection value of common tags. The apparatus is mainly used in the quantitative detection of residual soybean allergens in food safety.

Description

technical field [0001] The invention belongs to the field of detection of soybean protein in food in food safety, and in particular relates to a device for qualitatively and quantitatively rapidly detecting fluorescent microspheres of soybean protein in food and a preparation method thereof. Background technique [0002] In recent years, with the improvement of people's living standards, food safety issues have become more and more prominent, including food allergies. Food allergy refers to an allergic reaction of the body to a certain substance or component in food. It is a hypersensitivity reaction mediated by IgE and non-IgE, which can cause reactions in the skin, gastrointestinal tract, and respiratory system. In severe cases, it may even lead to systemic allergic reactions or shock. [0003] Soybean products are rich in protein and balanced amino acid components. They are high-quality plant protein sources for humans and livestock, but they are also one of the eight ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68
CPCG01N33/68G01N21/6402G01N33/533
Inventor 赖卫华倪小琴刘道峰山珊彭涛
Owner NANCHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap