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Compound rhizobium inoculant as well as preparation method and application thereof

A technology of composite rhizobia and bradyrhizobium, which is applied in the fields of application, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of non-controlling crop pests and restricting the popularization and application of rhizobia, so as to increase yield, The effect of efficient control

Active Publication Date: 2014-10-22
BIOTECH CENT OF SHANDONG ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As we all know, although rhizobia can fix nitrogen and promote growth, they have no ability to control crop pests, which restricts the popularization and application of rhizobia.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Cultivation of Bradyrhizobium BASWS and Bacillus thuringiensis BtR05 in peanut

[0035] Bradyrhizobium peanut BASWS medium is YMA medium, the formula is 10 g sucrose, 1 g yeast powder, MgSO 4 ·7H 2 O 0.2 g, K 2 HPO 4 0.5 g, NaCl 0.1 g, CaCl 2 0.05 g, sodium molybdate (0.5% solution) 4.0ml boric acid (0.5% solution) 4.0ml, H 2 O 1 000 mL, pH 6.8-7.0.

[0036] BtR05 slant and shake flask medium is LB. The fermentation medium is: 2% corn flour, 6% soybean flour, 1% sucrose, 0.5% (NH4) 2SO4, 0.2% K2HPO4, 0.2% KH2PO4, 0.03% MgSO4??7H2O, pH7.0-7.5.

[0037] (2) Toxicity determination of bradyrhizobium BASWS and Bacillus thuringiensis BtR05 on grubs

[0038] The 3rd instar larvae of grubs were treated by spraying method.

[0039] Spraying method: spray different concentrations of Bacillus thuringiensis BtR05 and Bradyrhizobium peanut BASWS all over the grub body with a hand-held sprayer, and after allowing it to crawl freely for a while, move it into a canned bott...

Embodiment 2

[0058] A compound rhizobia agent comprising the following ingredients by weight:

[0059] Peat soil 75.7~87.62%

[0060] Ammonium molybdate 0.5~1.0%

[0061] Peanut seedling powder 0.5~2.0%

[0062] γ-polyglutamic acid 0.05~0.5%

[0063] Sucrose 0.2~1.0%

[0064] Calcium carbonate 0.1~0.5%

[0065] Silicone 0.03~0.3%

[0066] Peanut bradyrhizobium BASWS1.0~4.0%

[0067] Bacillus thuringiensis BtR0510.0~15.0%.

[0068] Preparation and application

[0069] In the present invention, peat soil is used as carrier, ammonium molybdate, peanut seedling powder, γ-polyglutamic acid, sucrose, calcium carbonate, and organic silicon are used as synergistic aids, and the fermentation broth of Bradyrhizobium peanut BASWS and Bacillus thuringiensis BtR05 Made into powder, the effective number of bacteria is (Peanut Bradyrhizobium BASWS1-400 million / g, Bacillus thuringiensis BtR05 600 million / g)

[0070] Preparation method of bacterial agent: inject the fermented bacterial liquid into...

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PUM

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Abstract

The invention discloses a compound rhizobium inoculant as well as a preparation method and application thereof. The preparation method of the compound rhizobium inoculant comprises that turfy soil is taken as a carrier, ammonium molybdate, peanut seedling powder, gamma-polyglutamic acid, cane sugar, calcium carbonate and organic silicon are taken as synergist auxiliaries, and bradyrhizobium sp arachis (BASWS) and bacillus thuringiensis BtR05 fermentation liquor are made into powder, wherein effective bacterial count of the BASWS in the powder is 0.1-0.4 billion / g, and the effective bacterial count of the BtR05 is 0.6 billion / g. The compound rhizobium inoculant can be used for effectively preventing and controlling white grubs and can also be used for obviously increasing peanut yield.

Description

technical field [0001] The invention relates to the technical field of preparation of biological bacteria agents, in particular to a compound rhizobia agent and its preparation and application. Background technique [0002] Peanuts are grown all over the country, mainly distributed in Liaoning, Shandong, Hebei, Henan, Jiangsu, Fujian, Guangdong, Guangxi, Sichuan and other provinces (regions). Among them, Shandong Province has the largest planting area and the largest output. [0003] In recent years, with the continuous expansion of peanut sowing area, the harm of underground pests has become more and more serious. Among them, grubs are currently the biggest pests that harm peanuts. According to the survey, grubs cause an annual loss of more than 20% of the peanut yield, serious or even extinct (Zhou Shuai, Su Ruifeng, et al. Peanut underground pests - comprehensive control technology of grubs [J]. Seed Industry Guide, 2009 .). Bacillus thuringiensis ( Bacillus thuringi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P7/04C12R1/01C12R1/07
Inventor 李纪顺王贻莲魏艳丽刘伟陈凯杨合同
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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