Compound rhizobium inoculant as well as preparation method and application thereof
A technology of composite rhizobia and bradyrhizobium, which is applied in the fields of application, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of non-controlling crop pests and restricting the popularization and application of rhizobia, so as to increase yield, The effect of efficient control
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[0033] Example 1
[0034] Study on the Coexistence and Symbiosis of Peanut Bradyrhizobium BASWS and Bacillus thuringiensis BtR05
[0035] (1) Cultivation of Bradyrhizobium peanut BASWS and Bacillus thuringiensis BtR05
[0036] Peanut bradyrhizobia BASWS medium is YMA medium, the formula is 10 g sucrose, 1 g yeast powder, MgSO 4 ·7H 2 O 0.2 g, K 2 HPO 4 0.5 g, NaCl 0.1 g, CaCl 2 0.05 g, sodium molybdate (0.5% solution) 4.0 ml boric acid (0.5% solution) 4.0 ml, H 2 O 1 000 mL, pH 6.8~7.0.
[0037] BtR05 slant and shake flask medium is LB. Fermentation medium is: 2% corn meal, 6% soybean meal, 1% sucrose, 0.5% (NH4)2SO4, 0.2% K2HPO4, 0.2% KH2PO4, 0.03% MgSO4?? 7H2O, pH7.0-7.5.
[0038] (2) Toxicity of Bradyrhizobium peanut BASWS and Bacillus thuringiensis BtR05 to grubs
[0039] The third instar larvae of grubs were treated by spraying.
[0040] Spraying method: spray different concentrations of Bacillus thuringiensis BtR05 and Bradyrhizobium peanut BASWS over the grub...
Example Embodiment
[0057] Example 2
[0058] Synergistic mixed dose preparation of Bradyrhizobium peanut BASWS and Bacillus thuringiensis BtR05
[0059] A compound rhizobia agent, comprising the following components by weight:
[0060] Peat soil 75.7~87.62%
[0061] Ammonium molybdate 0.5~1.0%
[0062] Peanut seedling powder 0.5~2.0%
[0063] γ-Polyglutamic acid 0.05~0.5%
[0064] Sucrose 0.2~1.0%
[0065] Calcium carbonate 0.1~0.5%
[0066] Silicone 0.03~0.3%
[0067] Peanut bradyrhizobia BASWS1.0~4.0%
[0068] Bacillus thuringiensis BtR0510.0~15.0%.
[0069] Preparation and Application
[0070] The invention uses peat soil as a carrier, ammonium molybdate, peanut seedling powder, γ-polyglutamic acid, sucrose, calcium carbonate and organic silicon as synergists, and the fermentation broth of peanut brachyrhizobia BASWS and Bacillus thuringiensis BtR05 Made into powder, the number of effective bacteria is (Blagenrhizobium peanut BASWS1~400 million / g, Bacillus thuringiensis BtR05 600 mi...
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