Method for rapidly detecting enterohemorrhagic Escherichia coli

Abstract

The invention discloses a method for rapidly detecting enterohemorrhagic Escherichia coli. The method comprises the following steps: preparing an immune antigen, immunizing mice, purifying an antibody, and making a colloidal gold test strip. The method for rapidly and simply detecting enterohemorrhagic Escherichia coli is provided to make the highly-sensitive enterohemorrhagic Escherichia coli colloidal gold test strip. The enterohemorrhagic Escherichia coli in a sample can be directly detected, so the long standing problems of long detection period and complex detection operation of the enterohemorrhagic Escherichia coli are solved. Additionally, the detection method adopting an immune colloidal gold chromatographic analysis technology, a nanometer colloidal gold technology and an immune principle has the advantages of simple operation, convenience, rapidity, accuracy and sensitivity, is suitable for onsite rapid detection, and benefits for the monitoring of detection departments.

Description

technical field [0001] The invention belongs to the field of microorganism detection, in particular to a rapid detection method for enterohemorrhagic Escherichia coli. Background technique [0002] Escherichia coli is a Gram-negative short bacterium with a length of about 2*10-3cm, a width of about 0.5*10-3cm, and a volume of about 0.6-0.7*10-6cm. There are about 4-6 flagella in each bacterial cell. Flagella motile without spores. Escherichia coli has a strong ability to synthesize and metabolize, and it grows on ordinary medium containing inorganic salts, salts, and glucose. Hygienic standards for Escherichia coli exist in the intestines of humans and other warm-tempered animals in large quantities, and are often excreted together with human and animal feces , widely spread in the natural environment and pollute water sources. If detected in water and food, it can be considered as an indicator of fecal contamination. Escherichia coli is more resistant to heat than other ...

AI Technical Summary

Problems solved by technology

[0030] The purpose of the embodiments of the present invention is to provide a rapid detection method for enterohaemorrhagic Escherichia coli, aiming to prepare a high-sensitivity enterohaemorrhagic Escherichia coli colloidal gold test method by adopting the immunocolloidal gold chromatography analysis method and nano colloidal gold technology combined with the principle of immunity. Paper strips can directly detect enterohaemorrhagic Escherichia coli in the sample, which solves the long-standing problems of long-standing enterohaemorrhagic Escherichia coli detection cycle and complicated detection operation

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