Method for rapidly identifying broomrape resistance level of sunflowers

Abstract

The invention discloses a method for rapidly identifying the broomrape resistance level of sunflowers, and relates to the field of plant breeding. The method comprises the steps that inoculum is prepared; an incubator is prepared through cultivation soil and the inoculum; sunflower seeds are sown in the incubator permeated by water; observation seedlings of the sunflowers are obtained after indoor cultivation; the number of roots, infected by broomrape, of the observation seedlings is obtained through observation and recorded; the broomrape resistance level of the sunflowers is identified according to the number of the roots, infected by the broomrape, of the observation seedlings. According to the method, the sunflowers used for identifying the broomrape resistance level are sunflower seedlings with 8-10 main leaves, so that cultivation of sunflowers required by identification is short in time and high in efficiency, and rapid identification of the broomrape resistance level of the sunflowers is realized; besides, indoor cultivation is adopted, consumption of manpower and material resources is small, the cost is low, the workload of field identification is reduced greatly, the breeding cost is reduced, the breeding efficiency is improved, and a basis is provided for selection and popularization of new varieties of broomrape-resistant sunflowers.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a method for quickly identifying the level of sunflower resistance to Lidan. Background technique [0002] Sunflower Liedang (Orobanche cumana Wallr.) is a fully parasitic herbaceous plant with a plant height of 20-50cm, erect stems, degenerated leaves into scales, no chlorophyll; no real roots, and parasitizes on Sunflower root tissue; capsule, seeds irregular in shape, as fine as powder, each plant can reach 30,000 to 80,000 seeds, and the weight of a single seed is about 3g to 6g. Sunflowers should be propagated by seeds, mainly overwinter in the soil, and have strong vitality. They can survive in the soil for 10-15 years and become the main source of primary infection in the coming year. [0003] Sunflower Liedang is a parasitic weed that is seriously harmful to sunflowers. It is parasitic on the roots of sunflowers. After sunflower is parasitic by Liedang, the plant grows slow...

AI Technical Summary

Problems solved by technology

At present, the identification of the level of sunflower resistance to Lietang is usually carried out through field experiments. Since the field growth period of sunflower and Lietang is long and the coverage in the field is large, the sunflower resistance to the sunflower through field experiments When the level of identification method takes a long time, low efficiency, and consumes a lot of manpower and material resources, high cost

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