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Method for determining concentration of volatile anesthetic in sample through chromatography

A measurement method and volatility technology, applied in the field of biomedicine, can solve the problems of long measurement time and inconvenient storage, and achieve the effects of accurate results, reduced pollution, and shortened sample detection time

Active Publication Date: 2014-12-10
YICHANG HUMANWELL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method overcomes the disadvantages of cumbersome standard preparation and sample processing, long measurement time, and inconvenient storage in the prior art, and provides a simple, fast, efficient, accurate, and capable of widely processing a large number of samples for the determination of the concentration of volatile anesthetics method and its application in clinical monitoring

Method used

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  • Method for determining concentration of volatile anesthetic in sample through chromatography
  • Method for determining concentration of volatile anesthetic in sample through chromatography
  • Method for determining concentration of volatile anesthetic in sample through chromatography

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0038] The present invention will be further described in conjunction with specific examples, but not limited thereto. The mensuration of volatile anesthetic concentration in blood of embodiment 1

[0039] (1) Chromatographic conditions Agilent6890N gas chromatograph, DB-WAX capillary chromatographic column, inlet temperature 160°C, carrier gas high-purity nitrogen, column flow rate 2ml / min. The temperature is programmed to rise, starting at 60°C for 2 minutes, rising to 200°C at a speed of 10°C / min, maintaining for 2 minutes, and running for 18 minutes in total. The detector is a flame ionization detector (FID), and the detector temperature is 300°C. Automatic headspace sampling, heat preservation at 85°C for 30min, quantitative loop temperature at 90°C, transfer line temperature at 120°C, injection volume 1ml. (same chromatographic conditions)

[0040] (2) Preparation of standard stock solution Take an appropriate amount of sevoflurane reference substance, dissolve and di...

Embodiment 2

[0046] Example 2 The isoflurane concentration of the same whole blood sample was determined by automatic headspace sampling method and syringe headspace secondary balance method.

[0047] Experimental operation

[0048] Take two blank whole blood samples of 20ml each, add a certain amount of isoflurane reference substance respectively, and wait for one day to equilibrate at room temperature 25°C to obtain blood samples 1 and 2 containing isoflurane, and draw blood samples 1 and 2 using the secondary balance method of a syringe , to determine the concentration of isoflurane; another blood sample 1, 2 each 1ml was added to the headspace bottle and sealed with a cap, and this method was used to determine the concentration of isoflurane.

[0049] Experimental results

[0050] Table 3 Determination of isoflurane concentration in the same whole blood sample by automatic headspace sampling method and syringe headspace secondary equilibrium method

[0051]

[0052] (Accuracy is the...

Embodiment 3

[0054] The mensuration of volatile anesthetic concentration in embodiment 3DMF aqueous solution

[0055] Take an appropriate amount of volatile anesthetic, weigh it accurately, add DMF aqueous solution to dissolve and dilute to make a stock solution, and use the stock solution to double-dilute to 6-10 solutions of different concentrations as the solution to be tested. Take 0.5-1ml respectively and put them in the headspace bottle. Seal the bottle; measure the peak areas of samples with different concentrations under the chromatographic conditions of Example 1 for comparison. Use the linear regression of the peak area (Y-axis) of the volatile anesthetic isoflurane to the concentration (X-axis), draw a standard curve, and obtain the regression equation

[0056] Y=1.5969X+4.1027 R2=0.9994 The results show that the linearity is good, which is in line with the "Technical Guidelines for Clinical Pharmacokinetic Research of Chemical Drugs". See figure 1 .

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Abstract

The invention discloses a method for determining the concentration of a volatile anesthetic through an automatic headspace gas chromatography. The method is suitable for determining the concentrations of volatile anesthetics such as methoxyflurane, enflurane, isoflurane, sevoflurane and desflurane in samples. The samples can be solution containing a to-be-determined object and can also be in-vitro biological samples containing to-be-determined objects, such as blood, urine and tissue fluid. The method is convenient, rapid and accurate, and is suitable for determining and analyzing laboratory samples and determining a large number of samples in clinical monitoring.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for measuring the concentration of volatile anesthetics in a sample by automatic headspace gas chromatography. Background technique [0002] The final purpose of volatile anesthetics is exactly the same as that of general intravenous anesthetics, but when administered by inhalation, they must first reach a certain concentration in the anesthesia loop system, and then absorb into the blood through the lungs through the concentration partial pressure difference, and then pass through the circulation. The system is distributed to the surrounding tissues and reaches the central nervous system to exert its anesthetic effect, so the blood concentration of volatile anesthetics such as methoxyflurane, enflurane, isoflurane, sevoflurane and desflurane is measured is a very critical indicator. [0003] Liu Jin, Liu Mingzheng, etc. (determination of inhaled anesthe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/24
Inventor 易斌罗楠刘进张文胜袁瑛
Owner YICHANG HUMANWELL PHARMA
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