A method for setting the positive control of the ames test of the water extract of cigarette filter

Abstract

The invention relates to a positive control setting method of a cigarette filter tip water extract Ames test. The method comprises (1) selecting sample group cigarette filter tips to be tested and cigarette filter tips for preparing a positive control group of the same quantity; (2) preparing a direct black 38 water solution at a concentration of 20 mg / mL; (3) uniformly spraying the direct black 38 water solution onto the cigarette filter tips for preparing the positive control group, and drying the cigarette filter tips for preparing the positive control group naturally for standby; (4) extracting the positive control group filter tips and the sample group filter tips of the same quantity through the same method; (5) performing the Ames test on obtained water extract. By means of positive control set through the method, the reliability of the extracting process of the cigarette filter tips and the subsequent Ames test performed on the cigarette filter tip water extract can be confirmed.

Description

technical field [0001] The invention belongs to the technical field of tobacco, and in particular relates to a method for setting a positive control when performing an Ames test on a cigarette filter water extract. Background technique [0002] The Salmonella reverse mutation test (Ames test), established in 1975, is a method for rapidly detecting the mutagenicity of compounds. The histidine-deficient (his-) strain of Salmonella typhimurium cannot grow in the medium lacking histidine, but under the action of the mutagen, the bacteria can undergo a large number of back mutations, forming a self-synthesizing histidine wild-type (his+) strain, and can produce visible colonies on the medium lacking histidine. Therefore, it can be determined whether the test substance is mutagenic by counting the number of back-changing colonies in the plate. It is often used as one of the safety biological detection methods for food, medicine, dental materials, etc. [0003] Due to the direct...

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Problems solved by technology

[0005] Yet existing method is not suitable for the cytotoxicity test of cigarette filter tip extract, and this is because carrying out Ames test to cigarette filter needs to prepare cigarette filter extract, therefore, use above-mentioned first method to set positive control then Unable to verify that the extracted process was reliable

And for using the second method, since the current existing cigarette filter varieties are not found to be mutagenic, and there is no mutagenic material similar to the filter properties, in order to verify the extraction process of the cigarette filter and the follow-up Whether the Ames test on the cigarette filter water extract is reliable, it is necessary to develop a positive control setting method for the cigarette filter water extract Ames test

[0006] Through literature search, do not find the public report of the same literature as the content of the present invention

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