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A method for extracting Juema polysaccharides by ultrasonic-assisted degreasing

An ultrasonic and polysaccharide technology, applied in the field of medicine and chemical industry, can solve the problems of high cost, low yield and long time consumption

Inactive Publication Date: 2016-08-10
段玉虎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the shortcomings of existing extraction methods such as long time consumption, large amount of solvent, low yield and high cost, and to study the anti-fatigue and fatigue recovery of Juema polysaccharides by using ultrasonic-assisted degreasing to extract Juema polysaccharides role, providing valuable reference for scientific sports

Method used

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  • A method for extracting Juema polysaccharides by ultrasonic-assisted degreasing
  • A method for extracting Juema polysaccharides by ultrasonic-assisted degreasing

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Weigh 200.00g of fern basilisk dry powder, put it in a 1000ml flask, add 400ml of petroleum ether, reflux and degrease at 70°C for one hour, evaporate the solvent, add 400ml, 50% ethanol, soak and wash twice at room temperature, 4h each time, evaporate to dry Add 200ml of distilled water after the solvent, transfer to ultrasonic extraction equipment, extract with ultrasonic at 40°C and power of 80W for 20min, filter, collect the water extract, add water to the filter residue for ultrasonic extraction twice, combine the water extract, concentrate to 200ml by rotary evaporation, and add to it Add 2ml of 10μg / ml papain solution for enzymolysis at 40°C for 10h, then centrifuge the enzymolysis solution at 1000r / min for 5 minutes, take the supernatant, and filter it with a micro-suction filter (pressure 0.05MPa), further Remove the finer filter residue, concentrate the collected filtrate, and then use a mixture of n-butanol and chloroform with a volume ratio of 1:2 to extract ...

Embodiment 2

[0031] Weigh 200.00g of fern japonica dry powder, put it in a 1000ml flask, add 400ml of petroleum ether, reflux and degrease at 80°C for one hour, evaporate the solvent, add 400ml, 70% ethanol to soak and wash at room temperature twice, each time for 4h, evaporate the solvent Then add 200ml of distilled water, transfer to ultrasonic extraction equipment, 40 ℃ ultrasonic, power 80W extraction for 20min, filter, collect the water extract, add water to the filter residue for ultrasonic extraction twice, combine the water extract, concentrate to 200ml by rotary evaporation, add 2ml of 10μg / ml papain solution was enzymatically hydrolyzed at 40°C for 10h, then the enzymolyzed solution was centrifuged at 1000r / min for 5 minutes, the supernatant was taken and filtered with a micro-suction filter (pressure 0.05) to further remove fine filter residue, concentrate the collected filtrate, then extract 5 times with n-butanol and chloroform mixed solution with a volume ratio of 1:4, collect...

Embodiment 3

[0033]Weigh 200.00g of fern basilisk dry powder, put it in a 1000ml flask, add 400ml of petroleum ether, reflux and degrease at 70°C for one hour, evaporate the solvent, add 400ml, 90% ethanol to soak and wash at room temperature twice, each time for 4h, evaporate the solvent Then add 200ml of distilled water, transfer to ultrasonic extraction equipment, 40 ℃ ultrasonic, power 160W extraction for 40min, filter, collect the water extract, add water to the filter residue for ultrasonic extraction twice, combine the water extract, concentrate to 200ml by rotary evaporation, add 2ml of 10μg / ml papain solution was enzymolyzed at 40°C for 10h, then the enzymolyzed solution was centrifuged at 1000r / min for 5 minutes, the supernatant was taken, and filtered with a micro-suction filter (pressure 0.05, to further remove finer The filter residue was concentrated, and the filtrate collected was concentrated, and then extracted 5 times with a mixture of n-butanol and chloroform with a volum...

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Abstract

The invention provides a method for extracting Juema polysaccharides by ultrasonic-assisted degreasing. It takes Juema as raw material, adds petroleum ether, refluxes and degreases at different temperatures, evaporates the solvent, soaks and washes with ethanol at room temperature, adds distilled water, and transfers to ultrasonic Extraction equipment, ultrasonic extraction and filtration, collect the water extract, add water to the filter residue and ultrasonically extract twice, combine the water extract, concentrate by rotary evaporation, add papain enzymatic hydrolysis, take the supernatant after centrifugation, and filter with a micro-suction filter , further remove the finer filter residue, concentrate the collected filtrate, and then use a mixture of n-butanol and chloroform to extract 3 to 5 times, collect the upper aqueous phase, add ethanol to the aqueous phase, and centrifuge to collect the precipitate after standing, freeze Drying will give purified Bracken polysaccharide.

Description

technical field [0001] The invention relates to the technical field of medicine and chemical industry, in particular to a method for extracting juema polysaccharide by ultrasonic-assisted degreasing. Background technique [0002] Bracken is a unique economic crop on the Qinghai-Tibet Plateau, often used as health care products and Tibetan medicine. Consuming Juma series foods can eliminate fatigue, restore physical strength, improve anti-stress ability and anti-hypoxia ability, and regulate human immune function. The medicinal active ingredients of Bracken Bracken depend on the polysaccharides, phenolic acids and flavonoids contained in Bracken Bracken, so the extraction of these active ingredients is very important for the medicinal value of Bracken Bracken. [0003] At present, there are many reports on the extraction methods of Bracken. Bacteria polysaccharides are water-soluble, so the commonly used method is water extraction and alcohol precipitation, which specifical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P39/00A61P39/06
Inventor 段玉虎田晓柱
Owner 段玉虎
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