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Up-conversion composite material modified by cyclodextrin derivatives and its preparation method and application

A composite material, cyclodextrin technology, applied in analytical materials, luminescent materials, material excitation analysis, etc., can solve the problem of unreported cholesterol-selective fluorescent probes, eliminate background fluorescence interference, improve signal-to-noise ratio, The effect of increasing sensitivity

Inactive Publication Date: 2016-10-05
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, to the best of our knowledge, no cholesterol-selective fluorescent probes for UCNPs have been reported.

Method used

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  • Up-conversion composite material modified by cyclodextrin derivatives and its preparation method and application
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  • Up-conversion composite material modified by cyclodextrin derivatives and its preparation method and application

Examples

Experimental program
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Embodiment 1

[0032] Example 1. Preparation of Upconversion Composite Materials Modified by Cyclodextrin Derivatives

[0033] Step 1. With 1mmol RECl 3 (0.78mmol YCl 3 ,0.2mmol YbCl 3 ,0.02mmol ErCl 3 ) as the reaction raw materials, add 7.5mL oleic acid and 17.5mL 1-octadecene, stir evenly, and heat the solution to 160°C for 30min. Cool to room temperature, add 4mmol NH 4 F and 2.5mmol NaOH in methanol solution, stirred for 30min. Methanol was evaporated, and the solution was heated to 300° C. under the protection of argon, reacted for 1 h, and cooled to room temperature. The reaction solution was precipitated by adding ethanol, centrifuged, washed with water and ethanol several times to obtain a white precipitate, namely OA-UCNPs;

[0034] Step 2. Disperse 100 mg of OA-UCNPs in 10 mL of aqueous solution, add 0.1 M hydrochloric acid solution dropwise until pH = 4, and stir at room temperature for 4 h. Centrifuge and wash several times with ethanol aqueous solution to obtain ligand-f...

Embodiment 2

[0038] Example 2. Preparation of Upconversion Composite Materials Modified by Cyclodextrin Derivatives

[0039] The up-conversion composite material modified by cyclodextrin derivatives was prepared according to the steps of Example 1, but NH 4 The amount of substance F was 1.6 and 2.8 mmol, respectively, and the other steps were the same. Result is with embodiment 1.

Embodiment 3

[0040] Example 3. Preparation of Upconversion Composite Materials Modified by Cyclodextrin Derivatives

[0041] The upconversion composite material modified by cyclodextrin derivatives was prepared according to the steps of Example 1, but in step 1, the solution was heated to 280° C. under the protection of argon, and the other steps were the same. Result is with embodiment 1.

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Abstract

A method for preparing upconversion composite materials modified by cyclodextrin derivatives, which is to add oleic acid-wrapped upconversion nanoparticles (OA-UCNPs) dropwise into HCl solution to obtain UCNPs without oleic acid ligands. CD‑Cit-encapsulated UCNPs (CD‑Cit‑UCNPs) were obtained by reacting citric acid-modified β‑cyclodextrin (CD‑Cit) with UCNPs without oleic acid ligands at high temperature, CD‑Cit‑UCNPs and RB After assembly, an upconversion composite material modified by cyclodextrin derivatives was obtained. The up-conversion composite material of the present invention is based on a FRET process, uses UCNPs as energy donors, rhodamine B (RB) as energy acceptors, and utilizes the competitive reaction between host and guest. After adding cholesterol, cholesterol induces RB to dissociate from the cavity of β-cyclodextrin, resulting in the weakening of FRET and the enhancement of up-conversion fluorescence, so as to detect the content of cholesterol. The invention discloses its preparation method.

Description

technical field [0001] The invention relates to an up-converting composite material modified by cyclodextrin derivatives, a preparation method thereof and its application to the detection of cholesterol in serum. Background technique [0002] Cholesterol plays an important role in the evolution of many diseases, and the detection of cholesterol in serum is the most widely used analytical index in biochemistry. A balanced cholesterol content in the human body can ensure the durability and integrity of the cell structure, and the level of total cholesterol in the serum is an important indicator for medical diagnosis and treatment. (See: S.R. Cao, L. Zhang, Y.Q. Chai and R. Yuan, Biosens. Bioelectron., 2013, 42, 532-538.) [0003] Regarding cholesterol detection, many methods have been developed, such as fluorescence analysis, electrochemical analysis, and molecular imprinting techniques. However, these methods are limited by time-consuming, lack of suitable internal standard...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L5/16C08B37/16C09K11/85C09K11/02G01N21/64G01N21/31G01N21/33G01N21/3563
Inventor 朱俊杰丁玉洁张晓霞朱浩
Owner NANJING UNIV
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