Up-conversion composite material modified by cyclodextrin derivatives and its preparation method and application

A composite material, cyclodextrin technology, applied in analytical materials, luminescent materials, material excitation analysis, etc., can solve the problem of unreported cholesterol-selective fluorescent probes, eliminate background fluorescence interference, improve signal-to-noise ratio, The effect of increasing sensitivity
CN104231335BInactive Publication Date: 2016-10-05NANJING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING UNIV
Publication Date
2016-10-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method for preparing upconversion composite materials modified by cyclodextrin derivatives, which is to add oleic acid-wrapped upconversion nanoparticles (OA-UCNPs) dropwise into HCl solution to obtain UCNPs without oleic acid ligands. CD‑Cit-encapsulated UCNPs (CD‑Cit‑UCNPs) were obtained by reacting citric acid-modified β‑cyclodextrin (CD‑Cit) with UCNPs without oleic acid ligands at high temperature, CD‑Cit‑UCNPs and RB After assembly, an upconversion composite material modified by cyclodextrin derivatives was obtained. The up-conversion composite material of the present invention is based on a FRET process, uses UCNPs as energy donors, rhodamine B (RB) as energy acceptors, and utilizes the competitive reaction between host and guest. After adding cholesterol, cholesterol induces RB to dissociate from the cavity of β-cyclodextrin, resulting in the weakening of FRET and the enhancement of up-conversion fluorescence, so as to detect the content of cholesterol. The invention discloses its preparation method.
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Description

technical field

[0001] The invention relates to an up-converting composite material modified by cyclodextrin derivatives, a preparation method thereof and its application to the detection of cholesterol in serum. Background technique

[0002] Cholesterol plays an important role in the evolution of many diseases, and the detection of cholesterol in serum is the most widely used analytical index in biochemistry. A balanced cholesterol content in the human body can ensure the durability and integrity of the cell structure, and the level of total cholesterol in the serum is an important indicator for medical diagnosis and treatment. (See: S.R. Cao, L. Zhang, Y.Q. Chai and R. Yuan, Biosens. Bioelectron., 2013, 42, 532-538.)

[0003] Regarding cholesterol detection, many methods have been developed, such as fluorescence analysis, electrochemical analysis, and molecular imprinting techniques. However, these methods are limited by time-consuming, lack of suitable internal standard...

Claims

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