Time-resolved fluoroimmunoassay detection and copeptin kit construction process

Abstract

The invention discloses a lateral flowimmunoassay (LFIA) and time-resolved fluoroimmunoassay (TRFI) detection and copeptin (CPP) kit construction process. The kit comprises a test paper card, whereina sample pad, a combination pad, a nitrocellulose film and a water adsorbing pad are sequentially adhered to a bottom plate, and then a housing is added to form the test paper card; rare earth elementfluorescent microspheres are adsorbed to the combination pad to mark an anti-CPP antibody; a CPP antibody (detection line) and an anti-IgG antibody (quality control line) are sprayed to the nitrocellulose film; the diameter of the rare earth element fluorescent microspheres is 100-500nm, and the rare earth element fluorescent microspheres are capable of transmitting fluorescence being 615nm in wavelength under the effect of exciting light being 420nm in wavelength; and the fluorescence strength is detected through a supporting TRFIA analyzing instrument. The kit has the advantages of being simple and convenient to operate, fast to act, high in sensitivity, and high in specificity; the kit is used for detecting CPP concentration of blood or liquid samples and early determining cerebrovascular embolism.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] With the advent of an aging society, the incidence of cerebrovascular diseases is increasing, and the mortality and disability rates are high. Cerebrovascular infarction is the second cause of human death worldwide. The mortality rate within one year after the onset of cerebrovascular infarction is 21% to 27%, while 15% to 30% of survivors left long-term disabilities. The main method to reduce the incidence of cerebrovascular infarction is early prevention, early diagnosis, and early treatment. At present, there is no hematological index that can be used for early diagnosis of cerebrovascular infarction. Studies have found that in the early stage of cerebrovascular infarction, the level of Copeptin (CPP) in blood is significantly increased, and it is necessary to vigorously develop a kit for detecting CPP in blood for early diagnosis of cerebrovascular infarction. Con...

AI Technical Summary

Problems solved by technology

The main method to reduce the incidence of cerebrovascular infarction is early prevention, early diagnosis, and early treatment. At present, there is no hematological index that can be used for early diagnosis of cerebrovascular infarction.

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