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A kind of sulfhydryl ion nanoprobe material and its preparation method and application

A nanoprobe, hydrogen sulfide technology, applied in nanotechnology, nanotechnology, analytical materials, etc., can solve the problems of inability to detect hydrogen sulfide, poor photostability, toxicity, etc., to eliminate background fluorescence interference, good dispersion, Small particle size effect

Active Publication Date: 2017-12-05
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to improve the existing technical problems, such as high toxicity, inability to detect hydrogen sulfide in living cells, poor photostability, etc., to provide a sulfhydryl ion nanometer with good water solubility and long luminescent life Probe material and its preparation method, and its application in biological detection and imaging

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  • A kind of sulfhydryl ion nanoprobe material and its preparation method and application
  • A kind of sulfhydryl ion nanoprobe material and its preparation method and application
  • A kind of sulfhydryl ion nanoprobe material and its preparation method and application

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Effect test

Embodiment 1

[0033] Embodiment 1. A kind of sulfhydryl ion nanoprobe material, the specific preparation route is as follows:

[0034] S1. Preparation of gold nanoclusters (Au@MUA) with undecylmercaptoalkanoic acid as ligand:

[0035] 1. Add 13.1mg 11-MUA and 200μL 1M NaOH into 20mL water and dissolve completely.

[0036] 2. Add 510 μL (1% by mass) HAuCl4 and 445 μL THPC (80% aqueous solution, 12 μL water is added to 1 mL water to dissolve), the solution is stirred at room temperature for 2 hours, and the solution turns yellow.

[0037] 3. Add 10 mL of 500 mM NaCl aqueous solution to the obtained product, centrifuge at 6000 g for 15 min, remove the supernatant, and wash the precipitate twice with water and ethanol (V water: V ethanol = 2:1) to obtain the final purified product.

[0038]

[0039] S2. Modified chitosan molecules on the obtained Au@MUA to obtain gold nanoclusters (Au@MUA@CSa):

[0040] The Au@MUA 2mL solution obtained above was activated by adding 0.25mL 0.05M EDC and 0.2...

Embodiment 2

[0045] Example 2. Morphological characterization

[0046] Samples were prepared respectively according to the above specific steps, and the sample solution was dropped on the copper grid, and after drying, the morphology test was carried out, such as figure 2, it can be found that the particle size of various gold nanoclusters prepared is 1-2nm, and the dispersibility is very good.

Embodiment 3

[0047] Embodiment 3. Spectral test

[0048] The fluorescence emission spectrum test of the gold nanocluster material on the covalent bond modification to the sulfhydryl ion: take 200ul of PBS buffer solution dispersed with the gold nanocluster in the cuvette, gradually add the sulfhydryl ion (NaHS dissolved in PBS solution), such as image 3 Fluorescence emission spectra were measured.

[0049] Test the response of gold nanocluster materials modified by covalent bonds to different anions: take 200uL PBS buffer solution dispersed with gold nanoclusters in a cuvette, add 2uL 10 -2 M of F - , Cl - 、Br - , NO 3 - , NO 2 - , oAC - 、SCN - 、HPO 4 - , SO 4 2- , CO 3 2- , S 2 o 3 2- , HClO 4 - 、H 2 o 2 , NO, Gly and 2uL 10 -1 M Hcy, Cys, GHS, HS - 、GHS+HS - 、Cys+HS - , the measured fluorescence emission spectrum as Figure 4 .

[0050] According to the response spectrum of gold nanocluster materials modified by covalent bonds to sulfhydryl, it can be seen t...

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Abstract

The invention discloses a sulfhydryl group ion nanometer probe material as well as a preparation method and an application thereof. First, gold nano-cluster is prepared by a reduction method, wherein 11-mercaptoundecanoic acid is used as a ligand of the gold nano-cluster; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) are used for activating the gold nano-cluster, chitosan molecules with many amino molecules are combined, an amidation reaction is carried out, and a layer of chitosan molecules are covered outside the gold nano-cluster; finally, unreacted amino molecules on the chitosan are used, an amidation reaction is carried out, fluorescent micromolecules with response to sulfhydryl group ions are modified on the surface of the gold nano-cluster by a covalent bond mode, and the sulfhydryl group ion nanometer probe with better water-solubility is obtained. The sulfhydryl group ion nanometer probe material has a small and uniform particle size, detection of sulfhydryl group value in solutions and cells is realized, and the application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of nano-luminescent materials, and in particular relates to a sulfhydryl ion nano-probe material and its preparation method and its application in biological detection and imaging. Background technique [0002] Under normal circumstances, hydrogen sulfide is a colorless, flammable acidic gas. When the concentration is low, it has a foul smell, such as rotten eggs; At the same time, hydrogen sulfide is an acute poison. After inhaling high concentrations of hydrogen sulfide in a short period of time, tears, eye pain, and foreign body sensation in the eyes will appear. In severe cases, cerebral edema and pulmonary edema may occur. 3 Above) can suddenly coma within a few seconds, and lightning-type death occurs; and long-term low-concentration exposure can cause neurasthenia syndrome and autonomic dysfunction. However, studies have found that hydrogen sulfide exists widely in mammals and has important cytoprote...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06B82Y30/00B82Y20/00G01N21/64
CPCB82Y20/00B82Y30/00C09K11/06G01N21/6402
Inventor 赵强黄维杜静余琦刘淑娟许文娟
Owner NANJING UNIV OF POSTS & TELECOMM
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