A device and method for rapid and instant separation of human skin epidermal cells, melanocytes and fibroblasts

Abstract

The invention relates to the field of medical cell therapy, in particular to a device and a method for rapidly and immediately separating epidermal cells, melanophore and fibroblast of human skin. The separating device comprises a micro magnetic heating stirrer, an operating panel, digestive enzyme and an isotonic electrolyte solution. According to the separating method, instruments required for cell separation of the human skin are remarkably reduced, the operation steps are simplified, time required for cell separation is remarkably shortened, application of serum is avoided, occurrence of serious complication such as allergy, pathogenic microorganism propagation and the like is remarkably reduced, and wide popularization of human skin cell transplantation application is facilitated. According to the device and the method, separated cells can be used for scar plastic surgery, treatment of abnormal pigment change such as vitiligo and scar hypopigmentation caused by various reasons and acute and chronic wound repair.

Description

technical field [0001] The present invention relates to the field of medical cell therapy, in particular to a device for rapidly and instantly separating epidermal cells, melanocytes and fibroblasts from human skin, as well as a separation method; the cells separated by the present invention can be used for scar shaping and various reasons Treatment of abnormal pigment changes (such as vitiligo, cicatricial hypopigmentation) and repair of acute and chronic wounds. Background technique [0002] With the development of medical technology, cell therapy has become one of the important treatment methods for many diseases, and has attracted extensive attention from researchers and clinicians. The main cell components in human skin are epidermal cells, fibroblasts and melanocytes. The above cells are isolated and applied immediately (that is, without in vitro culture and expansion), or delayed application (that is, in vitro culture and expansion), combined with other treatments Th...

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Problems solved by technology

The above-mentioned method has many steps, takes a long time, and requires special instruments and equipment. It is difficult to perform synchronously with the operation in the operating room, which has become the main obstacle to the popularization and application of human skin cell therapy. Or cold digestion, the whole cell separation process takes 2-16 hours, and the separated cells are difficult to be applied in the operating room in a short time; 2. There are many steps to separate the cells: the first digestion is required to separate the epidermis, and the second digestion is required to separate the epidermis Cell components, then filter, centrifuge, resuspend cells, etc. The operation is relatively complicated, requires certain equipment and related technologies, and can only be carried out in laboratories with certain experimental conditions and technical personnel, and it is difficult to promote clinical application. ;3. There is a risk of severe complications of allergies and pathogenic microorganisms such as viruses, mycoplasma, and chlamydia: residual trypsin in the cell suspension needs to be added with trypsin inhibitors or serum to stop trypsin activity to avoid aggravating cell damage, allogeneic or heterogeneous The composition of serum is complex, which can lead to serious complications such as allergies and even anaphylactic shock, and there is a risk of spreading pathogenic microorganisms that are difficult to detect; 4. The separation methods used are different according to the conditions and experience of each laboratory, and there is no uniform Process and quality control standards, clinical application effect is unstable

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