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A kind of preparation method of botrytis cinerea agent

A technology for Botrytis cinerea and spore inoculants, applied in the field of microorganisms, can solve the problems of unstable spore culture effect, unfavorable large-scale production, inconvenient production and use, etc., and achieves the effects of significant economic benefits, reduced production costs, and stable effects

Inactive Publication Date: 2017-07-21
GRAPE & WINE RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Potato-sucrose-agar medium or potato-glucose-wort-agar medium and other laboratory-grade spore-producing mediums for preparing Botrytis cinerea species are not only expensive, but the effect of cultivating spores is unstable, and the production and use Inconvenient, not conducive to mass production

Method used

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  • A kind of preparation method of botrytis cinerea agent

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Comparison scheme
Effect test

Embodiment 1

[0030] 1) Preparation of solid medium

[0031] The lees produced by wine production and filtration are mixed with bran at a ratio of 1:0.75, and the water content of the solid medium substrate is controlled to be 60%, and then 0.5% of the total mass of colloidal chitosan is added to the solid substrate according to the mass ratio, to Inhibit the growth of miscellaneous bacteria, and promote the differentiation of Botrytis cinerea mycelium to produce spores. Finally, the matrix was thoroughly mixed and heated to 100°C for 60 minutes to sterilize.

[0032] 2) Inoculation with Botrytis cinerea

[0033] A. Preparation of seed medium: crush 200g of potatoes to small pieces with a diameter of no more than 1cm, crush 200g of barley malt to a particle size of 20 mesh, add 1L of tap water, saccharify at 60°C for 8 hours, filter through a 40-mesh filter or sieve to remove residue, and filtrate Make up to 1L volume with tap water, and sterilize with damp heat at 121°C for 20 minutes; ...

Embodiment 2

[0040] Embodiment 2: the preparation of Botrytis cinerea agent of the present invention

[0041] 1) Preparation of solid medium

[0042] The lees produced by wine production and filtration are mixed with bran in a ratio of 1:1, and the water content of the solid medium substrate is controlled to be 58%, and then 0.05% of the total mass of colloidal chitosan is added to the solid substrate according to the mass ratio, to Inhibit the growth of miscellaneous bacteria, and promote the differentiation of Botrytis cinerea mycelium to produce spores. Finally, the matrix was thoroughly mixed and heated to 100°C for 50 minutes to sterilize.

[0043] 2) Inoculation with Botrytis cinerea

[0044] A. Preparation of seed medium: crush 300g of potatoes to small pieces with a diameter of no more than 1cm, crush 300g of barley malt to a particle size of 20 mesh, add 1.5L of tap water, saccharify at 60°C for 8 hours, filter through a 40-mesh filter or sieve to remove residue, The filtrate i...

Embodiment 3

[0051] Embodiment 3: the preparation of Botrytis cinerea agent of the present invention

[0052] 1) Preparation of solid medium

[0053] The lees and bran produced by wine production and filtration are mixed in a ratio of 1:1.25, and the water content of the solid medium substrate is controlled to be 55%, and then 0.1% of the total mass of colloidal chitosan is added to the solid substrate according to the mass ratio, to Inhibit the growth of miscellaneous bacteria, and promote the differentiation of Botrytis cinerea mycelium to produce spores. Finally, the matrix was thoroughly mixed and heated to 100°C for 40 minutes to sterilize.

[0054] 2) Inoculation with Botrytis cinerea

[0055] A. Preparation of seed medium: crush 200g of potatoes to small pieces with a diameter of no more than 1cm, crush 200g of barley malt to a particle size of 20 mesh, add 1L of tap water, saccharify at 60°C for 8 hours, filter through a 40-mesh filter or sieve to remove residue, and filtrate Make...

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Abstract

The invention relates to the field of microorganisms and provides a preparation method of a botrytis cinerea agent. The preparation method comprises the following steps: (1) preparation of a solid-state culture medium; (2) inoculation of botrytis cinerea; (3) solid-state fermentation; (4) spore collection. The botrytis cinerea agent is produced by utilizing solid-state fermentation of wine lees, the wine lees can be recycled, and the production cost of the botrytis cinerea agent is greatly reduced; meanwhile, the physiological activity of the prepared botrytis cinerea agent is stable, the spore content of the botrytis cinerea agent reaches 10<8>-10<10>CFU / g, and the method is suitable for large-scale production operation.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for preparing a botrytis cinerea agent. Background technique [0002] Botrytis cinerea ( Botytis cinerea ) is a common pathogenic fungus of grapes, in general it can cause Botrytis cinerea infecting grape plants. But in the special environment of cold and humid in the morning and evening, and dry and hot in the afternoon, noble rot will be triggered. Noble rot is caused by grapes infected with Botrytis cinerea ( Botytis cinerea ), the infection and growth of Botrytis cinerea can volatilize the water in grape raw materials, leading to changes in the sugar, organic acid, polyols and other components in the grape fruit, and finally forming expensive grapes with high sugar content and strong aroma. Rotten grapes. The noble rot wine made from noble rot grapes is the most high-grade sweet wine in the world. [0003] Potato-sucrose-agar medium or potato-glucose-wort-agar medi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 文仁德李玮杨莹管敬喜文昊谢林君张劲
Owner GRAPE & WINE RES INST GUANGXI ACADEMY OF AGRI SCI
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