Molecule marker, screening method, kit and application for stress-resistant poplar

A technology of molecular markers and kits, applied in the field of molecular biology, can solve the problems of time-consuming difficulty and high cost, and achieve the effects of high viability, shortened selection cycle, and strong adaptability to adversity stress

Active Publication Date: 2015-01-21
BEIJING FORESTRY UNIVERSITY
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[0005] The present invention aims to provide a molecular marker, screening method, kit and application for screening stress-resistant poplars, so a

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  • Molecule marker, screening method, kit and application for stress-resistant poplar
  • Molecule marker, screening method, kit and application for stress-resistant poplar
  • Molecule marker, screening method, kit and application for stress-resistant poplar

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[0019] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0020] Using traditional breeding to select poplars with good stress resistance is time-consuming, difficult, costly, and poor in stability. In order to solve this technical problem, the present invention develops and screens molecular markers for stress-resistant poplars, which can identify resistant poplars at an early stage. A single plant with good resistance can greatly speed up the breeding process.

[0021] According to a typical embodiment of the present invention, a molecular marker for screening stress-resistant poplar is provided. The molecular marker is a molecular marker detected by the MSAP method, and the molecular marker includes base sequences of MS1 of SEQ ID NO.1, MS2 of SEQ...

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Abstract

The invention discloses a molecule marker, a screening method, a kit and an application for screening a stress-resistant poplar. The molecule marker is detected through an MSAP method and includes MS1, MS2, MS3, MS4, MS5, MS6, MS7, MS8, MS9 and MS10. By means of the molecule marker determined in the invention and for screening the stress-resistant poplar, plants which are strong in adversity stress adaptability and are high in vitality can be screened out in an early stage during growth of the poplars and a forest tree breeding selection period is greatly reduced. The invention has important strategic significance in development of a poplar fast-growing high-pulp-yield forest and of ensuring that a high-yield industrial pulp raw material can be provided stably and continuously in forestry in our country.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a molecular marker for screening stress-resistant poplar, a screening method, a kit and an application. Background technique [0002] DNA methylation is one of the earliest discovered methods of gene epigenetic modification. It is a modification method that regulates genomic DNA at the transcriptional level. It participates in processes such as gene expression, biological growth and development, and stress response. Among them, Plays an important role in the regulation of gene expression. The most commonly used technology to study genome methylation is methylation sensitive amplified polymorphism (Methylation sensitive amplified polymorphism, MSAP). Different methylation sensitivities, the same sequence can amplify different bands to detect methylation levels and effectively distinguish the methylation status of genomic DNA. [0003] Poplar is the main afforestation t...

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 张德强次东宋跃朋田敏周大凌
Owner BEIJING FORESTRY UNIVERSITY
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