Molecule marker, screening method, kit and application for stress-resistant poplar

A technology of molecular markers and kits, applied in the field of molecular biology, can solve the problems of time-consuming difficulty and high cost, and achieve the effects of high viability, shortened selection cycle, and strong adaptability to adversity stress
CN104293775AActive Publication Date: 2015-01-21BEIJING FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING FORESTRY UNIVERSITY
Publication Date
2015-01-21

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Abstract

The invention discloses a molecule marker, a screening method, a kit and an application for screening a stress-resistant poplar. The molecule marker is detected through an MSAP method and includes MS1, MS2, MS3, MS4, MS5, MS6, MS7, MS8, MS9 and MS10. By means of the molecule marker determined in the invention and for screening the stress-resistant poplar, plants which are strong in adversity stress adaptability and are high in vitality can be screened out in an early stage during growth of the poplars and a forest tree breeding selection period is greatly reduced. The invention has important strategic significance in development of a poplar fast-growing high-pulp-yield forest and of ensuring that a high-yield industrial pulp raw material can be provided stably and continuously in forestry in our country.
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Description

technical field

[0001] The invention relates to the technical field of molecular biology, in particular to a molecular marker for screening stress-resistant poplar, a screening method, a kit and an application. Background technique

[0002] DNA methylation is one of the earliest discovered methods of gene epigenetic modification. It is a modification method that regulates genomic DNA at the transcriptional level. It participates in processes such as gene expression, biological growth and development, and stress response. Among them, Plays an important role in the regulation of gene expression. The most commonly used technology to study genome methylation is methylation sensitive amplified polymorphism (Methylation sensitive amplified polymorphism, MSAP). Different methylation sensitivities, the same sequence can amplify different bands to detect methylation levels and effectively distinguish the methylation status of genomic DNA.

[0003] Poplar is the main afforestation t...

Claims

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