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Method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae

A technology of active ingredients and flavonoids, applied in the field of natural medicine extraction and detection, to achieve the effect of improving environmental protection benefits and reducing pollution

Active Publication Date: 2015-01-21
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Up to now, the matrix solid-phase dispersion extraction technology based on the trace system has not been reported in the whole field of analytical chemistry, and molecular sieves are used as the adsorbent, combined with the matrix solid-phase dispersion extraction technology, there is no such thing as extraction and detection of the active ingredients of traditional Chinese medicine. According to reports, this method is a blank for the research field of extraction and detection of traditional Chinese medicine tangerine peel

Method used

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  • Method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae
  • Method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae
  • Method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae

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preparation example Construction

[0048] The specific steps of the preparation method of the tangerine peel reference substance solution are as follows: respectively take an appropriate amount of reference substance of hesperidin, nobietin, and tangeretin, accurately weigh them, mix them in a brown measuring bottle, add methanol to make each 1 mL of hesperidin 500 μg, nobiletin 500 μg, and orange peeletin 500 μg solution, namely the reference substance solution.

[0049] The molecular sieve adsorbent used in the examples of the present invention was purchased from Nanjing Xianfeng Nano Material Technology Co., Ltd., and the model is SBA-15.

Embodiment 1

[0051] Weigh 25 mg each of 4 parts of tangerine peel medicinal powder and add them into 4 groups of mortars respectively, and weigh 25 mg each of different kinds of adsorbents (molecular sieve, Florisil, C18 reversed-phase silica gel, alumina), and add them respectively. Grind with tangerine peel medicinal powder for 150s in 4 groups of mortars. Take four 1mL solid-phase extraction cartridges, add sieves to the bottom, fill the cartridges with ground solids, fill the fillers, and add sieves to the top. Place the filled 4 small columns on the solid phase extraction apparatus. Take 0.5mL of methanol, inject it into the small column, turn on the air pump of the solid phase extraction apparatus, and elute the small column one by one. The bottom was collected with four 1.5mL centrifuge tubes respectively. After the elution, the liquid in each centrifuge tube was vortexed for 10s, then put into the centrifuge, and centrifuged at 13000rpm for 5min. The supernatant was taken, which...

Embodiment 2

[0060] Weigh 5 portions of tangerine peel medicinal powder, each 25 mg, and add them to 5 groups of mortars, and weigh molecular sieves of different masses (6.25 mg, 12.5 mg, 25 mg, 50 mg, 75 mg), add them to 5 groups of mortars to grind with tangerine peel medicinal powder 150s. Take five 1mL solid-phase extraction cartridges, add sieves to the bottom, fill the cartridges with ground solids, fill the fillers, and add sieves to the top. Place the filled 5 small columns on the solid phase extraction apparatus. Take 0.5mL of methanol, inject it into the small column, turn on the air pump of the solid phase extraction apparatus, and elute the small column one by one. The bottom was collected with five 1.5mL centrifuge tubes respectively. After the elution, the liquid in each centrifuge tube was vortexed for 10s, then put into the centrifuge, and centrifuged at 13000rpm for 5min. The supernatant was collected as a sample, and analyzed by ultra-high performance liquid chromatogr...

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Abstract

The invention discloses a method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae. The effective flavonoid components comprise one or more than two of aurantiamarin, nobiletin and hesperetin. The method comprises the following steps: mixing pericarpium citri reticulatae medicine powder and a molecular sieve absorbent according to a mass ratio of 1 to (0.25-3) so as to obtain a mixture, adding the mixture into a mortar for grinding to obtain ground solid, adding a sieve plate into the bottom of a small solid-phase extraction column, filling the small column with the ground solid, adding the sieve plate into the top of the small solid-phase extraction column after the ground solid is compacted, putting the compacted small column on a solid-phase extractor, injecting an eluent into the small column, starting an air extracting pump of the solid-phase extractor, eluting the small column, collecting the eluent by a centrifuge tube at the bottom, whirling and centrifuging the eluent in the centrifuge tube after the elution is ended, so as to obtain supernatant liquid as effective flavonoid component extraction liquid. According to the method, a molecular sieve is used as the absorbent; with the combination of a matrix solid-phase dispersion extraction technique, the effective flavonoid components in the traditional Chinese medicine pericarpium citri reticulatae can be extracted and detected in an efficient and environmental-friendly manner.

Description

technical field [0001] The invention belongs to the field of extraction and detection of natural medicines. It relates to a micro-extraction method of traditional Chinese medicine. Specifically, it relates to a new method for extracting trace amounts of flavonoid active ingredients in dried tangerine peel of traditional Chinese medicine by using molecular sieve as an adsorbent and combining matrix solid-phase dispersion extraction technology. Background technique [0002] Chenpi is the dry ripe pericarp of Rutaceae plant orange (Citrus reticulata Blanco) and its cultivars. Tangerine peel has the effects of drying dampness and resolving phlegm, regulating qi and invigorating the spleen, and its medicinal materials contain a large number of active ingredients. In addition to volatile oil, tangerine peel mainly contains flavonoids. The flavonoids can be divided into two types: flavonoid glycosides and polymethoxylated flavonoids. Among them, flavonoid glycosides are mainly d...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 曹君胡帅帅刘训高曹婉庞潇卿代菡镔达建华
Owner HANGZHOU NORMAL UNIVERSITY
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