Method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae

A technology of active ingredients and flavonoids, applied in the field of natural medicine extraction and detection, to achieve the effect of improving environmental protection benefits and reducing pollution

Active Publication Date: 2015-01-21
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Up to now, the matrix solid-phase dispersion extraction technology based on the trace system has not been reported in the whole field of analytical chemistry, and molecular sieves are used as the adsorbent, combined with the matrix solid-phase dispersion extract

Method used

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  • Method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae
  • Method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae
  • Method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae

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Experimental program
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Example Embodiment

[0048] The specific steps of the preparation method of the tangerine peel reference substance solution are as follows: take appropriate amounts of hesperidin, tangeretin, and hesperidin respectively, accurately weigh them, mix them in a brown measuring flask, and add methanol to make each lmL containing hesperidin A solution of 500μg, 500μg of Citrus citrifolia and 500μg of tangeretin is the reference solution.

[0049] The molecular sieve adsorbent used in the embodiment of the present invention was purchased from Nanjing Xianfeng Nano Material Technology Co., Ltd., and the model was SBA-15.

Example Embodiment

[0050] Example 1

[0051] Weigh 4 parts of dried tangerine peel medicinal powder each 25mg into 4 groups of mortars, and weigh 25mg of different kinds of adsorbents (molecular sieve, Florisil, C18 reversed-phase silica gel, alumina), and add them separately Grind the dried tangerine peel powder in a mortar for 150 seconds. Take 4 solid phase extraction cartridges of 1 mL size, add sieve plates to the bottom, respectively fill the small columns with ground solids, and add the top of the sieve plates to the top after filling the packing. Place the filled 4 small columns on the solid phase extraction instrument. Take 0.5 mL of methanol and inject it into the small column, turn on the suction pump of the solid phase extractor, and elute the small columns one by one. The bottom is collected with 4 1.5mL centrifuge tubes. After the elution, the liquid in each centrifuge tube was vortexed for 10 seconds, and then placed in a centrifuge, and centrifuged at 13000 rpm for 5 minutes. Ta...

Example Embodiment

[0059] Example 2

[0060] Weigh 5 portions of dried tangerine peel medicinal powder each 25mg into 5 groups of mortars, and weigh molecular sieves of different masses (6.25mg, 12.5mg, 25mg, 50mg, 75mg), add them to 5 groups of mortars and grind the dried tangerine peel medicinal powder 150s. Take 5 solid phase extraction cartridges of 1mL size, add sieve plates to the bottom, respectively fill the small columns with the ground solids, and add the top of the sieve plates after filling the packing. Place the filled 5 small columns on the solid phase extraction instrument. Take 0.5 mL of methanol and inject it into the small column, turn on the suction pump of the solid phase extractor, and elute the small columns one by one. The bottom is collected with 5 1.5mL centrifuge tubes. After the elution, the liquid in each centrifuge tube was vortexed for 10 seconds, and then placed in a centrifuge, and centrifuged at 13000 rpm for 5 minutes. Take the supernatant to load samples, and ...

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Abstract

The invention discloses a method for extracting effective flavonoid components in traditional Chinese medicine pericarpium citri reticulatae. The effective flavonoid components comprise one or more than two of aurantiamarin, nobiletin and hesperetin. The method comprises the following steps: mixing pericarpium citri reticulatae medicine powder and a molecular sieve absorbent according to a mass ratio of 1 to (0.25-3) so as to obtain a mixture, adding the mixture into a mortar for grinding to obtain ground solid, adding a sieve plate into the bottom of a small solid-phase extraction column, filling the small column with the ground solid, adding the sieve plate into the top of the small solid-phase extraction column after the ground solid is compacted, putting the compacted small column on a solid-phase extractor, injecting an eluent into the small column, starting an air extracting pump of the solid-phase extractor, eluting the small column, collecting the eluent by a centrifuge tube at the bottom, whirling and centrifuging the eluent in the centrifuge tube after the elution is ended, so as to obtain supernatant liquid as effective flavonoid component extraction liquid. According to the method, a molecular sieve is used as the absorbent; with the combination of a matrix solid-phase dispersion extraction technique, the effective flavonoid components in the traditional Chinese medicine pericarpium citri reticulatae can be extracted and detected in an efficient and environmental-friendly manner.

Description

technical field [0001] The invention belongs to the field of extraction and detection of natural medicines. It relates to a micro-extraction method of traditional Chinese medicine. Specifically, it relates to a new method for extracting trace amounts of flavonoid active ingredients in dried tangerine peel of traditional Chinese medicine by using molecular sieve as an adsorbent and combining matrix solid-phase dispersion extraction technology. Background technique [0002] Chenpi is the dry ripe pericarp of Rutaceae plant orange (Citrus reticulata Blanco) and its cultivars. Tangerine peel has the effects of drying dampness and resolving phlegm, regulating qi and invigorating the spleen, and its medicinal materials contain a large number of active ingredients. In addition to volatile oil, tangerine peel mainly contains flavonoids. The flavonoids can be divided into two types: flavonoid glycosides and polymethoxylated flavonoids. Among them, flavonoid glycosides are mainly d...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 曹君胡帅帅刘训高曹婉庞潇卿代菡镔达建华
Owner HANGZHOU NORMAL UNIVERSITY
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