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Detection method for compound Xueshuantong (thrombus clearing) preparation fingerprint chromatogram

A compound Xueshuantong and fingerprint technology, applied in the field of drug analysis, can solve the problem of inability to detect the active ingredients with strong representativeness at the same time, and achieve the guarantee of uniformity and stability, good column efficiency, and many peaks. Effect

Active Publication Date: 2015-01-21
GUANGDONG ZHONGSHENG PHARMA
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Problems solved by technology

[0004] The object of the present invention is to provide a detection method for the fingerprint of compound Xueshuantong preparation, aiming to solve the problem that the existing detection method cannot simultaneously detect the relatively strong representative activities of Astragalus, Salvia miltiorrhiza and Scrophulariaceae in the compound Xueshuantong preparation. ingredient question

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  • Detection method for compound Xueshuantong (thrombus clearing) preparation fingerprint chromatogram
  • Detection method for compound Xueshuantong (thrombus clearing) preparation fingerprint chromatogram
  • Detection method for compound Xueshuantong (thrombus clearing) preparation fingerprint chromatogram

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Embodiment 1

[0034] The investigation of embodiment one mobile phase

[0035] In order to screen out suitable mobile phase, the contriver uses need testing solution to investigate different mobile phases, carries out elution by the gradient in table 1, and the result is as follows Figure 1 to Figure 3 shown. Wherein, mobile phase 1: use acetonitrile as mobile phase A, water as mobile phase B; mobile phase 2: use acetonitrile as mobile phase A, and use 0.02% phosphoric acid solution as mobile phase B; mobile phase 3: use acetonitrile as mobile phase A, with 0.1% phosphoric acid solution as mobile phase B; mobile phase 4: with acetonitrile as mobile phase A, with 0.02% acetic acid solution as mobile phase B; mobile phase 5: with acetonitrile as mobile phase A, with 0.1% acetic acid solution as mobile phase mobile phase B. from Figure 1 to Figure 3It can be seen that when acetonitrile-0.1% phosphoric acid solution is used as the mobile phase, the baseline is relatively stable, and the re...

Embodiment 2

[0038] Example 2 Investigation of Mobile Phase Gradient

[0039] With acetonitrile as mobile phase A, 0.1% phosphoric acid solution as mobile phase B, use the test solution to investigate the gradient of mobile phase according to the gradient in Table 2 respectively, the results are as follows Figure 4 to Figure 6 shown. from Figure 4 to Figure 6 It can be seen that the time required for gradient 5 is short, and the resolution, peak shape and column efficiency are better.

[0040] Table 2 Elution gradient of mobile phase

[0041]

Embodiment 3

[0042] The investigation of embodiment three column temperature

[0043] With acetonitrile as mobile phase A, 0.1% phosphoric acid solution as mobile phase B, using the test solution to investigate the column temperature according to the gradient in Table 1, the results are as follows Figure 7 to Figure 8 shown. from Figure 7 to Figure 8 It can be seen that when the column temperature is 20°C, the separation effect of the test solution is better.

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Abstract

The invention belongs to the technical field of pharmaceutical analysis and provides a detection method for compound Xueshuantong (thrombus clearing) preparation fingerprint chromatogram. The detection method comprises the following steps: A) preparing a sample solution; B) preparing a reference substance solution; and C) detecting the fingerprint chromatogram of the compound Xueshuantong (thrombus clearing) preparation, namely, respectively extracting the reference substance solution and the sample solution and injecting into a liquid chromatographic instrument for detection, so as to obtain the fingerprint chromatogram of the compound Xueshuantong (thrombus clearing) preparation, wherein the detection conditions of the liquid chromatographic instrument comprise that the chromatographic column employs octadecylsilyl silica gel as a filler, acetonitrile is taken as a mobile phase A, a phosphoric acid solution with the volume percentage content of 0.1% is taken as a mobile phase B, gradient elution is performed, the detection wavelength is 270 nm and the column temperature is 18-22 DEG C. The provided detection method is capable of simultaneously detecting active compositions with relatively strong representativeness in radix astragali, salvia miltiorrhiza and scrophularia ningpoensis, is good in repeatability and strong in specificity, and is beneficial for guaranteeing the homogeneity and the stability of the product quality.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, in particular to a method for detecting the fingerprint of a compound Xueshuantong preparation. Background technique [0002] Fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum that can mark the chemical characteristics of some traditional Chinese medicinal materials or preparations after proper treatment and certain analysis methods. It has become an international consensus to use fingerprint as a quality control method for Chinese medicine extracts and their preparations. The fingerprint control technology system in line with the characteristics of Chinese medicine is one of the current research hotspots in the field of Chinese medicine. [0003] Compound Xueshuantong preparation is a pure traditional Chinese medicine preparation made from Panax notoginseng, Astragalus root, Salvia miltiorrhiza and Scrophulariae scrophulariae. Compound Xueshuantong prepara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 谢称石龙超峰杜静黄爱君
Owner GUANGDONG ZHONGSHENG PHARMA
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