Breeding method for an excellent line of flounder resistant to Edwardsiella blunt disease
A technology of flounder and flounder, which is applied in the field of selection and breeding of excellent strains of flounder resistant to Edwardsiella flounder, and can solve the problems of lack of breeding methods for excellent strains of flounder resistant to Edwardsiella flounder
Active Publication Date: 2016-01-27
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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[0004] The purpose of the present invention is to establish a method for breeding excellent strains of flounder resistant to tardy Edwardsiella, to make up for the deficiencies in the prior art, to provide a technical method for the cultivation of fine varieties of fish resistant to tardy Edwardsiella, and to solve the problem of flounder aquaculture. The problem of the lack of breeding methods for excellent strains resistant to tardy Edwardsiella in China, and to provide technical means for the breeding of excellent disease-resistant strains for the fish farming industry
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[0029] The present invention establishes a method for breeding excellent lines of Japanese flounder resistant to Edwards sluggish disease through long-term research, which mainly includes the following steps: Edwards sluggish infection of Japanese flounder fry and its disease resistance determination method, resistance to Edwards sluggish disease Population screening methods, screening methods for resistant families against Edwardsiella sluggish disease, and screening methods for specific microsatellite markers for resistant families resistant to Edwardsiella sluggish disease, etc., to screen specific microsatellite markers for resistant families; establish disease resistance and breeding of the offspring of resistant families The survival rate test method has created an excellent line of Paralichthys olivaceus against Edwardsiella slow.
[0030] The terms used in the present invention are explained as follows:
[0031] 1. Groups: mainly refer to different geographical groups and d...
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The invention discloses a method for breeding Edwardsiella tarda-resistant excellent flounder breed. The method mainly comprises the following steps: screening Edwardsiella tarda-resistant pedigrees specific microsatellite markers by an Edwardsiella tarda-infected flounder fry and disease resistance measuring method, an Edwardsiella tarda-resistant group screening method, an Edwardsiella tarda-resistant pedigrees screening method, an Edwardsiella tarda-resistant pedigrees specific microsatellite marker screening method and the like; establishing a disease-resistant pedigree offspring disease resistance and breeding survival rate measuring method, and establishing the Edwardsiella tarda-resistant excellent flounder breed. The flounder breed bred by the method has the characteristics of strong Edwardsiella tarda resistance, high breeding survival rate, low death rate and relatively high growth speed; the problem of massive death caused by an ascites disease due to the infection of Edwardsiella tarda in summer is basically solved; the method is suitable for being popularized and applied in flounder breeding farms in coastal areas in China.
Description
Technical field [0001] The invention belongs to the technical field of fish genetic breeding in the aquaculture industry, and specifically relates to a method for selecting and breeding excellent lines of Paralichthys olivaceus resistant to Edwardsiella slow. Background technique [0002] Paralich is an important mariculture fish in China, Japan and Korea, and it is also a favorite high-protein aquatic product. At present, the farming output of Chinese flounder is about 20,000 tons per year, with an output value of 2.5 to 3 billion yuan. In addition to meeting the needs of the domestic market, flounder is also one of the main aquatic products that are exported to earn foreign exchange. However, with the rapid development of the Paralichthys olivaceus aquaculture industry, some problems have emerged one after another, especially the frequent occurrence of diseases, the degradation of germplasm, and the low survival rate of seedlings, which cause nearly 1 billion yuan in economic ...
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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 陈松林张英平刘洋孙何军田永胜王磊范彩霞高峰涛沙珍霞王娜
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI