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Lipopeptid-kaolin composite biocontrol agent and preparation method and application thereof

The technology of biocontrol preparation and kaolin, which is applied in the field of microbial source pesticide preparation, can solve the problems of low compounding degree, poor stability and short effect time, and achieve the effect of prolonging action time, saving production cost and improving control effect.

Active Publication Date: 2015-02-04
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of high production cost, low output, poor stability, short time to exert effect and limited effective development and utilization of lipopeptide in the prior art. At the same time, it is also to solve the problem that the composite degree of biological composite materials is not high. To solve the problem that the tomato bacterial wilt control agent made of biocomposite materials is not effective, we provide a lipopeptide produced by the solid variable temperature fermentation of agricultural waste, and use exfoliated kaolin as the inorganic carrier to develop a control agent for tomato bacterial wilt Preparation method of novel biocontrol agent and the biocontrol agent

Method used

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  • Lipopeptid-kaolin composite biocontrol agent and preparation method and application thereof
  • Lipopeptid-kaolin composite biocontrol agent and preparation method and application thereof
  • Lipopeptid-kaolin composite biocontrol agent and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] The preparation method of lipopeptide-kaolin compound biocontrol preparation: (1) preparation of lipopeptide powder: Streak the frozen preserved Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain XZ-173 (preservation number CGMCCNO.4160) on the LB plate, Put it in an incubator for activation, pick a single colony and inoculate it in LB liquid medium, culture it at 30°C and 170rpm for 24 hours to obtain a fermented seed liquid; weigh 4.5kg of soybean powder, 3kg of straw, 0.15kg of starch and 0.15kg of Put the yeast extract into a 50L fermentation tank, add 1L of inorganic salt solution, adjust the pH of the substrate to 7, ensure the water content is 40%, set the speed at 50rpm and stir for 60min to fully mix the substrate and sterilize; among them, the inorganic salt solution Including (g / L):KH 2 PO 4 1.0, MgSO 4 ·7H 2 O 0.5, KCl 0.5, L-phenylalanine 2×10 -3 , MnSO 4 5×10 -3 , CuSO 4 0.16×10 -3 , FeSO 4 0.15×10 -3 After the temperature in the ...

Embodiment 2

[0044] The preparation method of lipopeptide-kaolin compound biocontrol agent: (1) preparation of lipopeptide powder: the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) bacterial strain XZ-173 that freezes preservation is streaked on LB plate, is placed in the incubator and activated, Pick a single colony and inoculate it in LB liquid medium, at 30°C, 170rpm

[0045] Cultivate under conditions for 24 hours to obtain fermented seed liquid; weigh 6kg of soybean powder, 4.5kg of straw, 0.2kg of starch and 0.2kg of yeast extract, put them into a 50L fermenter, add 2L of inorganic salt solution, adjust the pH of the substrate to 8, and contain water The rate is 60%, set the rotating speed to 70rpm and stir for 80min to fully mix the matrix and sterilize; wherein, the inorganic salt solution includes (g / L): KH 2 PO 4 1.0, MgSO 4 ·7H 2 O 0.5, KCl 0.5, L-phenylalanine 2×10 -3 , MnSO 4 5×10 -3 , CuSO 4 0.16×10 -3 , FeSO 4 0.15×10 -3 ; After the temperature in the ...

Embodiment 3

[0049] The preparation method of lipopeptide-kaolin compound biocontrol agent: (1) preparation of lipopeptide powder: the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) bacterial strain XZ-173 that freezes preservation is streaked on LB plate, is placed in the incubator and activated, Pick a single colony and inoculate it in LB liquid medium, cultivate it at 30°C and 170rpm for 24 hours, and use it as a fermented seed solution; weigh 5.67kg of soybean flour, 3.67kg of straw, 0.17kg of starch and 0.18kg of yeast extract, and put In a 50L fermenter, add 1L of inorganic salt solution, adjust the pH of the matrix to 7.5, and the water content to 55%, set the rotation speed to 70rpm and stir for 60 minutes to fully mix the matrix and sterilize; wherein, the inorganic salt solution includes (g / L): K H 2 PO 4 1.0, MgSO 4 ·7H 2 O 0.5, KCl 0.5, L-phenylalanine 2×10 -3 , MnSO 4 5×10 -3 , CuSO 4 0.16×10 -3 , FeSO 4 0.15×10 -3After the temperature in the fermenter i...

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Abstract

The invention belongs to the field of microbial pesticide preparation and provides a lipopeptid-kaolin composite biocontrol agent and a preparation method and an application thereof. The composite biocontrol agent is composed of lipopeptid, exfoliated kaolin and water, wherein mass percent of lipopeptid and exfoliated kaolin is 0.1wt% and 2wt% respectively. The preparation method of the lipopeptid-kaolin composite biocontrol agent comprises the following processes: (1) preparation of a lipopeptide powder; (2) preparation of exfoliated kaolin; and (3) acquisition of the lipopeptid-kaolin composite biocontrol agent. In the lipopeptid-kaolin composite biocontrol agent, stability of lipopeptide is enhanced by the combination of exfoliated kaolin and lipopeptide, action time of lipopeptide is prolonged, control efficiency of controlling tomato bacterial wilt is improved effectively, and plant growth of tomato is promoted. By the method, problems such as high production cost of lipopeptide, low output, poor stability, short effectiveness time, restricted effective development and utilization in the prior art are solved, and effects of a tomato bacterial wilt control agent prepared from the biological composite material are improved.

Description

technical field [0001] The invention belongs to the field of preparation of microbial source pesticides, and relates to a lipopeptide-kaolin compound biocontrol agent and its preparation method and application. Background technique [0002] Tomato bacterial wilt is a bacterial soil-borne disease caused by Ralstonia solanacearum which is widely distributed all over the world. In the early stage of the disease, the petioles of the lower leaves of the plant droop, and then the leaves gradually wither, and adventitious roots appear in the stems, and the plants wilt rapidly and gradually die. In mild cases, the production will be reduced by 20% to 40%, and in severe cases, it will reach more than 60% or even no harvest. There are large genetic variations in R. solanacearum, and there is no effective chemical control method at present. Commonly used control measures such as resistance breeding, field removal, crop rotation, and integrated management have little effect due to the...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/08A01P1/00
Inventor 吴彥良冉炜朱震孙立飞余光辉沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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