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Composite type intestinal-health regulating agent for groupers and preparation method thereof

A technology of intestinal health and grouper, which is applied in application, animal feed, animal husbandry, etc., can solve problems such as unstable use effects, and achieve the promotion of intestinal development, the improvement of intestinal digestive enzyme activity, and the regulation of intestinal bacteria The effect of group balance

Inactive Publication Date: 2015-02-04
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most of the intestinal health regulation products at home and abroad use a single type of additive, and the use effect is unstable. There is no commercialized compound intestinal health regulator based on the beneficial bacteria native to seawater fish. Research and development of related products are urgently needed

Method used

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  • Composite type intestinal-health regulating agent for groupers and preparation method thereof

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Effect test

Embodiment 1

[0033] A preparation method of a compound intestinal health regulator for grouper, which is specifically implemented in accordance with the following steps: fermenting probiotics, using an inoculation loop to inoculate Bacillus pumilus SE5 cells preserved on the slopes of two bacterial loops into 10 cells containing Culture in a 250mL Erlenmeyer flask with 50mL of medium 1, wherein the composition of medium 1 is: 5g of beef extract, 10g of peptone, 20g of glucose, 5g of sodium chloride, and 1000mL of distilled water; the culture conditions are: culture temperature 28°C; initial pH value 7.0; shaking table speed 210rpm; culture time is 24h; obtain 500mL primary seed culture solution; inoculate the primary seed culture solution into 25 2000mL Erlenmeyer flasks with 400mL medium 2 according to the ratio of 3%, wherein, Medium 2 was 5g of beef extract, 10g of peptone, 20g of glucose, 5g of sodium chloride, and 1000mL of distilled water; the culture conditions were: culture temperat...

Embodiment 2

[0046] A preparation method of a composite intestinal health regulator for grouper, which is specifically implemented in accordance with the following steps: fermenting probiotics, using an inoculation loop to inoculate Bacillus pumilus SE5 cells preserved on the slopes of two bacterial loops into 6 tubes containing 50mL culture medium 1 was cultured in a 250mL Erlenmeyer flask, wherein the composition of culture medium 1 was: beef extract 4g, peptone 12g, glucose 18g, sodium chloride 6g, distilled water 1000mL; culture conditions were: culture temperature 28°C; initial pH value 7.2; the rotation speed of the shaking table is 180rpm; the cultivation time is 24h; obtain 350mL of primary seed culture solution; inoculate the primary seed culture solution into 20 2000mL Erlenmeyer flasks containing 400mL medium 2 at a ratio of 4%, wherein, Medium 2 is 4g of beef extract, 12g of peptone, 18g of glucose, 6g of sodium chloride, and 1000mL of distilled water; the culture conditions are...

Embodiment 3

[0048]A preparation method of a composite intestinal health regulator for grouper, which is specifically implemented according to the following steps: fermenting probiotics, using an inoculation loop to inoculate 6-10 bacterial cells of Bacillus pumilus SE5 preserved on the slant of two bacterial loops Cultivate in a 250mL Erlenmeyer flask containing 50mL of medium 1, wherein the composition of medium 1 is: beef extract 6g, peptone 8g, glucose 25g, sodium chloride 4g, distilled water 1000mL; culture conditions are: culture temperature 30°C; The pH value is 7.2; the rotating speed of the shaking table is 240rpm; the cultivation time is 18h; the primary seed culture solution of 500mL is obtained; the primary seed culture solution is inoculated into 30 2000mL Erlenmeyer flasks containing 400mL medium 2 according to the ratio of 2%, Among them, medium 2 is 6g of beef extract, 8g of peptone, 25g of glucose, 4g of sodium chloride, and 1000mL of distilled water; the culture conditions...

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Abstract

The invention discloses a composite type intestinal-health regulating agent for groupers. The composite type intestinal-health regulating agent comprises the following components in parts by weight: 5-10 parts of probiotic powder, 20-25 parts of fructooligosaccharide, 5-10 parts of tributyrin, 15-20 parts of glutamine, 10-20 parts of white carbon black and 15-45 parts of defatted rice bran. The invention also discloses a preparation method of the composite type intestinal-health regulating agent for the groupers. The composite type intestinal-health regulating agent has the advantages that the development of the intestines of the groups can be enhanced, the intestinal mucosal damage is restored, the immunologic function of the intestinal mucosa and the immunologic function of the system are activated, the balance of the intestinal flora is regulated, the health of the intestinal mucosa is maintained, and the morbidity of the enteritis is reduced. In addition, the activity of intestinal digestive enzyme can be improved, and the growth performance of the groupers and the feed utilization efficiency are improved.

Description

technical field [0001] The invention belongs to the technical field of aquatic feed additives, and in particular relates to a compound intestinal health regulator for grouper, and also relates to a preparation method of the compound intestinal health regulator for grouper. Background technique [0002] The intestine is not only the main place for the digestion and absorption of nutrients in aquatic animals, but also an important immune organ, metabolic organ and endocrine organ in the body. Intestinal health is closely related to the health and production performance of aquatic animals. Once the intestinal function is disturbed, the digestion and absorption of animals will be hindered, the supply of nutrients will be insufficient, and the production performance of animals will decline. In addition, after intestinal function is damaged, pathogenic bacteria such as Vibrio are easy to invade, causing outbreaks of intestinal diseases such as bacterial enteritis. A large number ...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16
Inventor 孙云章杨红玲夏汉钦赵芸叶继丹
Owner JIMEI UNIV
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