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Identification method for key ribosomal proteins in developmental regulation of female schistosoma japonicum katsurada

The technology of a ribosomal protein and an identification method is applied in the identification field of the key ribosomal proteins for the developmental regulation of Schistosoma japonicum females, and can solve the problems of pathogenicity of live females, re-infection of patients, and lack of immune protection.

Inactive Publication Date: 2015-02-18
TONGJI UNIV
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  • Claims
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Problems solved by technology

In addition, after schistosomiasis is cured by existing drugs, patients can still be re-infected due to contact with infected water, and they do not have immune protection
Studies have shown that live worms in the human body can stimulate the body to produce immune protection against re-infection, but live females themselves can cause disease due to sexual maturity and laying eggs

Method used

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  • Identification method for key ribosomal proteins in developmental regulation of female schistosoma japonicum katsurada
  • Identification method for key ribosomal proteins in developmental regulation of female schistosoma japonicum katsurada
  • Identification method for key ribosomal proteins in developmental regulation of female schistosoma japonicum katsurada

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Embodiment

[0027] Gene expression profiles were analyzed using Solexa technology, as follows:

[0028] (1) The principle of obtaining monosexual cercariae by infecting one mouse with the cercariae released by one snail by using the characteristic of single snail releasing single sex cercariae.

[0029] (2) Infect 50 mice with at least 50 snails, and then infect at least 20 mice with a combination of cercariae released from different snails. Raise 18 days and 23 days in SPF class animal house. If only females are obtained from a single mouse, it can be used as a single-sex female sample; if a single mouse obtains hermaphrodites, the females are separated and used as a bisexual female sample.

[0030] (3) Females were collected on day 18 and day 23, respectively. Firstly, perfuse the mouse aorta with sterile pre-cooled normal saline, flush out the 18-day or 23-day schistosomiasis, and wash three times in sterile normal saline.

[0031] (4) Under a dissecting microscope, use a syringe ne...

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Abstract

The invention relates to an identification method for key ribosomal proteins in developmental regulation of female schistosoma japonicum katsurada. The identification method comprises the following steps: using schistosoma japonicum cerariae to respectively infect mice; breeding the infected mice in an SPF (Specific Pathogen Free) level animal house; respectively collecting monosexually and bisexually infected female schistosoma japonicum katsurada; respectively carrying out solexa analysis and iTRAQ analysis on the female schistosoma japonicum katsurada; contrasting the ribosomal protein expression profile and gene expression profile in four developmental stages to obtain the expressional characteristics of the ribosomal proteins in the two levels of proteins and genes in different developmental stages of the female schistosoma japonicum katsurada. Compared with the prior art, through the Solexa and iTRAQ combinatory analysis of the ribosomal gene expression profile and the protein expression profile of the female schistosoma japonicum katsurada before and after copulation of female and male schistosoma japonicum katsurada and in combination with real-time PCR and western technical identification, the key ribosomal proteins in the developmental regulation of female schistosoma japonicum katsurada can be effectively determined. The identification of proteins provides a key basis for the research and development of the control measures of interfering the development of the schistosoma japonicum katsurada in a host and maintaining the concomitant immune protection force of the host for the purpose of influencing the ribosomal function of the schistosoma japonicum katsurada.

Description

technical field [0001] The invention belongs to the field of life medicine sciences, and in particular relates to an identification method for a key ribosomal protein in the regulation and development of Schistosoma japonicum female. Background technique [0002] After schistosomiasis infects the human body, the production and release of a large number of eggs caused by sexual maturity is the root cause of schistosomiasis. In addition, after schistosomiasis is cured by existing drugs, patients can still be infected again due to contact with infected water, and they do not have immune protection. Studies have shown that live worms in the human body can stimulate the body to produce immune protection against re-infection, but live female worms themselves can cause disease due to sexual maturity and laying eggs. Therefore, there is an urgent need to establish a method that can regulate the development of schistosomiasis in the infected person, even if the infected person maint...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
CPCC12Q1/686G01N33/68C12Q2561/113
Inventor 孙军王素文
Owner TONGJI UNIV
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