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Method and system for acquiring age of female individual of Chinese population

A technology for women and individuals, applied in the fields of biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of improving the efficiency of solving cases and narrowing the scope of search

Active Publication Date: 2015-02-18
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the studies focus on European and American populations, and there are few studies on Chinese populations, especially female individuals in Chinese populations.

Method used

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  • Method and system for acquiring age of female individual of Chinese population
  • Method and system for acquiring age of female individual of Chinese population
  • Method and system for acquiring age of female individual of Chinese population

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 Using the method and system provided by the present invention to obtain the individual age of females in the Chinese population

[0054] Hereinafter, a female individual is taken as an example to illustrate the implementation process of the solution of the present invention.

[0055] 1. Extract the DNA of the individual to be tested as a template

[0056] The DNA solution of the above-mentioned one female individual was extracted separately using a DNA blood midi kit (QIAGEN, Hilden, Germany).

[0057] 2. Perform multiple PCR amplification reactions on the extracted DNA templates

[0058] 2.1. Primer pool configuration

[0059] The configuration of the amplification primer pool, wherein the amplification primer set is 11 pairs of amplification primers corresponding to the 11 CpG sites, and each pair of amplification primers can amplify the corresponding CpG on the DNA to be detected. site; in this embodiment, preferably, the amplification primer set of t...

Embodiment 2

[0084] Using the same method and system as in Example 1, the same steps were used to infer the ages of all 49 unrelated Chinese female individuals of different ages. To test the accuracy of the above system, the results are shown in the following table:

[0085]

[0086]

[0087] The age error range of the female individual obtained by the method of this embodiment is about 6.4 years old, indicating that the method and system of the present invention can be used to infer the age of individuals aged 20 to 80, or even over 80 years old, and has good accuracy, which can be Case detection provides data support.

[0088]

[0089]

[0090]

[0091]

[0092]

[0093]

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Abstract

The invention provides a method and a system for acquiring an age of a female individual of Chinese population. The method comprises the following steps: extracting DNA (Deoxyribonucleic Acid) of the female individual, acquiring methylation indexes of 11 CpG loci of the DNA, conducting regress analysis to the methylation indexes of each GpG loci, the 11 CpG loci and the age with SPSS 17 (Statistic Package for Social Science) software, and constructing a regression model to obtain the age of the female individual of the Chinese population. The scheme provided by the invention can accurately obtain the age of the female individual of the Chinese population, deduces the age of the individual with a biologically examined blood or blood stain sample remained in a crime scene of the public security actual combat, so as to facilitate targeting of the range of criminal suspects, provide clues for investigation of the case, and increase the detection speed of the case.

Description

technical field [0001] The present invention relates to a method and a system for obtaining the age of a Chinese population, in particular to a method and a system for obtaining the individual age of a female in a Chinese population. Background technique [0002] The estimation of individual age has always been one of the important research topics in forensic science. Currently, the assessment of individual age is mainly based on the temporal changes of the physical and chemical properties of tissues such as bones and teeth and various substances in the tissues with age. Due to the extremely complex physiological changes and influencing factors of the growth and development of various tissues and organs in organisms, different methods for inferring age have their application value, but they all have certain limitations. For example, 1) The bone age estimation of the six major joints used in the research of the Physical Evidence Center of the Ministry of Public Security is ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/154
Inventor 丰蕾徐程胡兰徐珍李万水
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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