Method for removing aflatoxin from feed through fermentation and preparation method of fermented feed

A technology for aflatoxin and fermented feed, which is applied in the field of microorganisms, can solve the problems of small damage to physical and chemical properties of nutrients, large damage to nutrients, low stability, etc. In addition to the highly selective effect

Inactive Publication Date: 2015-03-11
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The physical and chemical removal methods of aflatoxin have obvious effects, but the selectivity is poor, and the damage to other nutrients in the feed is relatively large, which lacks practical application and economic and technical feasibility; the biological method has little damage to the physical and chemical properties and nutritional componen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Candida utilis CGMCC 2.2066 is a liquid cryopreserved strain.

[0026] The seed medium of the strain is: 5 g of yeast extract, 20 g of peptone, 2.5 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 1000 mL of water, pH 5.5, and sterilized at 121° C. for 20 minutes.

[0027] Medium for expanded culture: 20 g of glucose, 15 g of yeast extract, 2.0 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 0.5 g of guaiacol, 1000 mL of water, pH 5.5.

[0028] The liquid volume of the seed medium was 50mL / 250mL Erlenmeyer flask, the inoculum volume was 0.2%, the culture temperature was 28°C, the shaker speed was 170r / min, and the culture was shaken at constant temperature for 3 days. The liquid volume of medium for expanded culture is 100mL / 500mL Erlenmeyer flask, the inoculum size is 0.2%, the culture temperature is 28°C, the shaker speed is 120r / min, the culture time is 5d, and the number of yeast in each milliliter of fermentation liquid is 5.8× 10 ...

Embodiment 2

[0033] Candida utilis (Candida utilis) CGMCC 2.2066 is a liquid cryopreserved strain.

[0034] The seed medium of the strain is: 5 g of yeast extract, 20 g of peptone, 2.5 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 1000 mL of water, pH 5.5, and sterilized at 121° C. for 20 minutes.

[0035] The medium for the expanded culture is: 20 g of glucose, 10 g of yeast extract, 2.0 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 0.3 g of guaiacol, 1000 mL of water, and pH 5.5.

[0036] The liquid volume of the seed medium was 50mL / 250mL Erlenmeyer flask, the inoculum volume was 0.2%, the culture temperature was 28°C, the shaker speed was 170r / min, and the culture was shaken at constant temperature for 3 days.

[0037] A 10L fully automatic fermenter was used to expand the culture of strains, the liquid volume during fermentation was 7L, the inoculum size was 70mL, the culture temperature was 25°C, the stirring speed was 200r / min, and the ventilation...

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PUM

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Abstract

The invention discloses a method for removing aflatoxin from feed through fermentation and a preparation method of fermented feed. According to the method, candida utilis CGMCC 2.2066 is inoculated into feed to be processed for fermentation so as to achieve removal of the aflatoxin from the feed. According to the method, through high-efficiency degradation of the candida utilis CGMCC 2.2066 to the aflatoxin, high-efficiency effect for biologically removing the aflatoxin from the feed is achieved.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for fermenting and removing aflatoxin in feed and a method for preparing fermented feed. Background technique [0002] Since 100,000 turkeys in the UK died of aflatoxin in 1960, aflatoxin has become a key monitoring object in the feed and food industry. Aflatoxins are a class of natural toxic substances produced by fungi such as Aspergillus flavus, including several structural analogues of aflatoxins B1, B2, G1, G2, M1 and M2; among them, aflatoxin B1 The most common and the most toxic. In 1993, the World Health Organization (WHO) defined aflatoxin as a Class 1 carcinogen, which mainly induces liver cancer and also has carcinogenic effects on the kidney, lung, and stomach. Livestock and poultry animals consume a large dose of aflatoxin at one time, which will cause acute poisoning and death; while the accumulation in the body after long-term small dose intake will lead to...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 李加友沈洁于建兴陆筑凤
Owner JIAXING UNIV
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