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A method for extracting and purifying phytosterols from corn silk

A technology for phytosterol and corn silk is applied in the field of extracting and purifying natural phytosterol, which can solve the problems of uneconomical economic benefit, complicated extraction process, low product recovery rate, etc., and achieve simple extraction equipment, short process flow and high product purity. Effect

Active Publication Date: 2016-04-06
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditionally, phytosterols are derived from vegetable oil residues and tall oil pitch obtained from the papermaking process. However, these extraction methods have defects such as high extraction temperature, complicated extraction process, long process, large solvent consumption, and low product recovery rate. The sterols produced in the world are far from meeting the needs of industrial production
Chinese patent CN102250198A discloses a method for extracting phytosterols from dragon fruit stems using supercritical fluid extraction technology, but this method consumes a lot of fluid and has high costs, which is very uneconomical from the perspective of economic benefits

Method used

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  • A method for extracting and purifying phytosterols from corn silk
  • A method for extracting and purifying phytosterols from corn silk
  • A method for extracting and purifying phytosterols from corn silk

Examples

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Effect test

Embodiment 1

[0024] A method for extracting and purifying phytosterols from corn silk, which comprises the following steps:

[0025] 1) Grind the removed corn silk with a grinder, pass through a 60-mesh sieve, and then dry it in an oven (80°C). Take 50g of crushed and dried corn silk and saponify with 100ml of saponification solution at 85°C for 3h, then add 250ml of absolute ethanol for ultrasonic extraction for 0.5h, centrifuge for 30min after ultrasonic extraction, take the supernatant, and rotary evaporate to obtain the crude extract. Described saponification liquid is the potassium hydroxide ethanol solution of concentration 3mol / L, is about to dissolve an appropriate amount of potassium hydroxide in 80V% ethanol to obtain saponification liquid;

[0026] 2) The crude extract was extracted with a mixed solution of water and ether at a volume ratio of 1:5, and the organic phase was rotary evaporated to obtain a concentrated extract, which was then recrystallized with ethyl acetate to ob...

Embodiment 2

[0028] A method for extracting and purifying phytosterols from corn silk, which comprises the following steps:

[0029] 1) Grind the removed corn silk with a grinder, pass through a 60-mesh sieve, and then dry it in an oven (80°C). Take 50g of crushed and dried corn silk and saponify with 300ml of saponification solution at 75°C for 4h, then add 300ml of absolute ethanol for ultrasonic extraction for 1h, centrifuge for 30min after ultrasonic extraction, take the supernatant, and rotary evaporate to obtain the crude extract. Described saponification liquid is the potassium hydroxide ethanol solution of concentration 4mol / L, is about to dissolve appropriate amount of potassium hydroxide in 90V% ethanol to obtain saponification liquid;

[0030] 2) The crude extract was extracted with a mixed solution of water and ether at a volume ratio of 1:6, and the organic phase was rotary evaporated to obtain a concentrated extract, which was then recrystallized from ethyl acetate to obtain ...

Embodiment 3

[0032] A method for extracting and purifying phytosterols from corn silk, which comprises the following steps:

[0033] 1) Grind the removed corn silk with a grinder, pass through a 60-mesh sieve, and then dry it in an oven (80°C). Take 50g of crushed and dried corn silk and saponify with 200ml of saponification solution at 90°C for 2h, then add 400ml of absolute ethanol for ultrasonic extraction for 0.5h, centrifuge for 30min after ultrasonic extraction, take the supernatant, and rotary evaporate to obtain the crude extract. Described saponification liquid is the potassium hydroxide ethanol solution of concentration 3mol / L, is about to be dissolved in an appropriate amount of potassium hydroxide in 85V% ethanol to obtain saponification liquid;

[0034] 2) The crude extract was extracted with a mixed solution of water and ether at a volume ratio of 1:8, and the organic phase was rotary evaporated to obtain a concentrated extract, which was then recrystallized with ethyl acetat...

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Abstract

The invention relates to a method for extracting and purifying phytosterols from corn silk, which comprises the following steps: 1) adding 1 g of crushed and dried corn silk to 2-6ml saponification liquid, saponifying at 85±10°C for 2-4 hours, and then Add absolute ethanol for ultrasonic extraction for 0.5-1h, centrifuge after ultrasonic extraction, take the supernatant and rotary evaporate to obtain the crude extract; the saponified solution is potassium hydroxide ethanol solution with a concentration of 2-5mol / L; 2) the crude extract is used After solvent extraction, it is recrystallized from ethyl acetate to obtain the finished product. The method of the invention has simple operation, short technological process, low production cost, high product yield and high purity (up to 83%), and opens up a new way for the full utilization of waste resource corn silk.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for extracting and purifying natural phytosterols from corn silk. Background technique [0002] my country's corn production ranks second in the world. As a by-product of corn, corn silk is a huge resource and is very easy to obtain, but its development and utilization are very limited. Except for a small amount of medicine, most of them are discarded in vain, which not only causes a lot of waste, And pollute the environment. Corn silk not only has obvious pharmacological value, but also has certain dietary characteristics, and has great prospects for industrial development and utilization. The development and research of corn silk is also conducive to the reuse of waste resources and meets the requirements of national resource utilization and environmental protection. [0003] Phytosterol is a natural active substance existing in plants. Sterol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 张海燕张杰薄冰张珅边涛葛泰宏曹晓婉
Owner HENAN UNIVERSITY
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