Application of wheat AX (arabinoxylan) extracts to reducing pH values of intestinal tracts and/or increasing contents of short-chain fatty acids in intestinal tracts

A technology of arabinoxylan and medium-short-chain fatty acids, which is applied to the application field of wheat arabinoxylan extract in reducing intestinal pH and/or increasing the content of short-chain fatty acids in the intestinal tract, and can solve the problem of large amount of flour raw materials , Difficult extraction, discharge of alkali-containing wastewater and other problems, to achieve the effect of increasing the content of short-chain fatty acids, simple and efficient operation, and reducing the pH value

Inactive Publication Date: 2015-03-25
THE QUARTERMASTER EQUIPMENT RESEARCH INSTITUTE OF THE GENERAL LOGISITIC DEPARTME
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Bran is a by-product of wheat processing, and the content of arabinoxylan is rich, about 20% or more. The annual output of bran in my country is as high as 20 million tons. However, most of the arabinoxylans in the bran are insoluble in water and difficult to extract. Currently, the feasible method is alkaline extraction. The alkaline extraction process will bring a large amount of alkali-containing wastewater to be discharged, causing environmental pollution
[0005] The content of arabinoxylan in flour is about 2%, of which the water-extractable part accounts for about 0.5% to 0.8%. Extracting arabinoxylan directly from flour requires a large amount of flour raw materials and high extraction costs. Currently, it is only in the experiment Small-scale extraction is carried out in the laboratory, and the extraction method is mostly physical centrifugation combined with enzymatic method, which is only used for scientific research, and the exploration of large-scale industrial production is not enough. There is no mature process route, and industrial production cannot be carried out.
[0006] Wheat starch and gluten production wastewater contains high-quality water-soluble arabinoxylan. The wheat flour processi

Method used

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  • Application of wheat AX (arabinoxylan) extracts to reducing pH values of intestinal tracts and/or increasing contents of short-chain fatty acids in intestinal tracts
  • Application of wheat AX (arabinoxylan) extracts to reducing pH values of intestinal tracts and/or increasing contents of short-chain fatty acids in intestinal tracts

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1, preparation wheat arabinoxylan extract

[0029] Centrifuge the production waste water of wheat flour after extracting gluten powder at 3000 rpm, take the supernatant after centrifugation; centrifuge the supernatant after cooking to obtain the supernatant, wherein, cooking The temperature is 80°C, the cooking time is 20 minutes, and the centrifugation condition is 2000 rpm; the obtained supernatant is spray-dried to obtain dry powder of wheat arabinoxylan extract, and the spray-drying temperature is 80°C .

[0030] The purity of the wheat arabinoxylan extract prepared in this example is 30% (that is, the mass percentage containing arabinoxylan).

Embodiment 2

[0031] Embodiment 2, preparation wheat arabinoxylan extract

[0032] Centrifuge the production waste water of wheat flour after extracting gluten powder at 3000 rpm, take the supernatant after centrifugation; centrifuge the supernatant after cooking to obtain the supernatant, wherein, cooking The temperature is 100°C, the cooking time is 70 minutes, and the centrifugation condition is 5000 rpm; the obtained supernatant is concentrated, and the concentration is 1 times; the pH value is adjusted to 7.5, and then β - Amylase and glucoamylase (both are 1% of the total mass of the system, and the mass ratio of the two is 1:1, the enzyme activity of β-amylase is 50000U / g, and the enzyme activity of glucoamylase is 50000U / g). Carry out the reaction, the reaction temperature is 85°C, and the reaction time is 60 minutes; the reaction solution after the reaction is centrifuged to obtain the supernatant, and the supernatant is precipitated with ethanol: the amount of ethanol used in the ...

Embodiment 3

[0034] Embodiment 3, preparation wheat arabinoxylan extract

[0035] The production wastewater of wheat flour after extracting gluten and wheat starch is centrifuged under the condition of 3000 rpm, and the supernatant after centrifugation is taken; the supernatant is centrifuged after cooking to obtain the supernatant, Among them, the cooking temperature is 900°C, the cooking time is 50 minutes, and the centrifugation condition is 4000 rpm; the obtained supernatant is concentrated, and the concentration is 1 times; and the pH value is adjusted to 7.5, Then add β-amylase and glucoamylase (1.5% of the total mass of the system, and the mass ratio of the two is 1:5, the enzyme activity of β-amylase is 100000U / g, and the enzyme activity of glucoamylase is 100000U / g g). Carry out the reaction, the reaction temperature is 85°C, and the reaction time is 60 minutes; the reaction solution after the reaction is centrifuged to obtain the supernatant, and the supernatant is precipitated ...

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Abstract

The invention discloses an application of wheat AX (arabinoxylan) extracts to reducing the pH values of intestinal tracts and/or increasing the contents of short-chain fatty acids in the intestinal tracts. The wheat AX extracts are prepared by a method comprising the following steps: carrying out centrifugal separation on industrial wastewater generated after extracting wheat gluten and/or wheat starch from wheat flour, and taking a supernatant; cooking the supernatant, and then carrying out centrifugal separation on the supernatant to obtain a supernatant; obtaining the wheat AX extracts by 1) carrying out spray drying on the supernatant, or 2) concentrating the supernatant, regulating the pH value to be 7-8, then adding beta-amylase and glucoamylase to react, finally obtaining a supernatant after centrifugal separation and precipitating the supernatant with ethanol. The extracts have the functions of reducing the pH values of the intestinal tracts and/or increasing the contents of the short-chain fatty acids in the intestinal tracts. Reduction of the pH values of the intestinal tracts is not beneficial to growth of potential pathogenic bacteria in the intestinal tracts, so that the extracts have positive effects on the functions of colons. Increase of the contents of the short-chain fatty acids is beneficial to the good micro-ecological environments of the intestinal tracts.

Description

technical field [0001] The invention relates to the application of a wheat arabinoxylan extract in reducing the pH value of the intestinal tract and / or increasing the content of short-chain fatty acids in the intestinal tract. Background technique [0002] Arabinoxylans (AX for short) is a hemicellulose that exists in the primary and secondary cell walls of plants, including grain seeds and wood. AX is a polymer composed of two sugars, xylose and arabinose, also known as pentosan. The basic structure includes the main chain of xylan and the side chain of arabinose, which are connected by xylose residues through 1,4-glycosidic bonds, and there are also phenolic acids connected by ester bonds on some arabinose. [0003] At present, there are two main sources of wheat arabinoxylan extraction, one is extracted from wheat bran, and the other is extracted from wheat flour. The extraction methods mainly include alkaline extraction and enzyme extraction. [0004] Bran is a by-prod...

Claims

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Application Information

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IPC IPC(8): C08B37/14C12P19/22C12P19/20C12P19/02A61K31/715A61P1/00
Inventor 刘晋钱平郭长江李凌燕王越鹏高蔚娜蒲玲玲王宇平
Owner THE QUARTERMASTER EQUIPMENT RESEARCH INSTITUTE OF THE GENERAL LOGISITIC DEPARTME
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