Regulating method of WDR63 gene in osteogenic differentiation and odontogenic differentiation processes of mesenchymal stem cell

A technology of WDR63 and mesenchymal stem cells, applied in genetic engineering, botany equipment and methods, biochemical equipment and methods, etc., can solve problems that do not involve WDR63 gene regulation, etc.

Active Publication Date: 2015-03-25
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a method for regulating the WDR63 gene in the osteogenic and odontogenic differentiation of mesenchymal stem

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  • Regulating method of WDR63 gene in osteogenic differentiation and odontogenic differentiation processes of mesenchymal stem cell
  • Regulating method of WDR63 gene in osteogenic differentiation and odontogenic differentiation processes of mesenchymal stem cell
  • Regulating method of WDR63 gene in osteogenic differentiation and odontogenic differentiation processes of mesenchymal stem cell

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[0025] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0026] Such as Figure 5 As shown, the method for regulating the WDR63 gene in the embodiment of the present invention during the osteogenic and odontogenic differentiation of mesenchymal stem cells comprises the following steps:

[0027] S501: Cell culture, after the wisdom teeth are disinfected with 75% alcohol, they are rinsed with phosphate buffered saline, and the stem cells of the apical papilla are isolated and cultured;

[0028] S502: Chromatin immunoprecipitation and data analysis, cells were incubated in 1% formaldehyde solution for 15 minutes, 2×10 6 3 cells and an anti-trimethylated antibody against histone 3 ...

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Abstract

The invention discloses a regulating method of a WDR63 gene in osteogenic differentiation and odontogenicdifferentiation processes of a mesenchymal stem cell. The cell is replanted by virtue of cell culture, chromatin immunoprecipitation reaction and data analysis, plasmid construction and virus infection, western blotting analysis, cell reproductive capacity determination and alkaline phosphatase and alizarin red dyeing subcutaneously of a nude mouse, and the regulating effect of the WDR63 gene in osteogenic differentiation and odontogenic differentiation processes of the mesenchymal stem cell and the effect of promoting regeneration of tooth tissues are found. By virtue of the adjusting effect of gene activity and function of the mesenchymal stem cell by trimethylation of lysine on the fourth site of histone 3, the regulating effect of the WDR63 gene in osteogenic differentiation and odontogenicdifferentiation processes of the mesenchymal stem cell and the effect of the WDR63 gene in promoting regeneration of tooth tissues, the WDR63 gene obtained probably has a promoting effect on osteogenic differentiation of stem cells from apical papilla.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for regulating WDR63 gene in the osteogenic and odontogenic differentiation of mesenchymal stem cells. Background technique [0002] Dental disease is one of the most common diseases in humans, and the loss of teeth greatly affects people's health and quality of life. Although the existing denture restoration methods are mature, they are difficult to compare with natural teeth due to their lack of biological activity, and cannot realize the vision of humans having a "third set of teeth". To this end, countries try to use stem cells and tissue engineering technology to achieve tooth reconstruction. How to develop a tooth that has biological activity and function and can rebuild the periodontal relationship in the jaw to restore missing teeth is a major issue that clinical dentists and scientific researchers expect to solve. With the development of ora...

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Application Information

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IPC IPC(8): C12N5/10C12N15/867
Inventor 范志朋刁树杨东梅王松灵
Owner BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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