DNA Barcoding-based identification kit for 9 normal fishes

Abstract

The invention discloses a DNA Barcoding-based identification method for 9 normal fishes (turbot, black carp, Chinese sturgeon, trachinotus blochii, trichiurus haumela, raw fish, the yellow river catfish, blunt-snout bream and gadus) and uncooked meat products of the 9 fishes. The 9 normal fishes and the uncooked meat products of the 9 normal fishes can be identified by comparing the lengths of edited base sequences. The method is established based on DNA Barcoding, is not limited by the morphology of identified materials, and can be widely used for accurately identifying fishes and uncooked meat products of the fishes as accurate conclusion can be made even when only individual tissue fragments are available.

Description

technical field [0001] The invention discloses a method for identifying fish, in particular to a DNA Barcoding-based identification method for 9 common fish species (turtlefish, herring, Chinese sturgeon, golden pomfret, hairtail, raw fish, Yellow River catfish, Wuchang fish, cod) identification method. Background technique [0002] As an indicator organism of the aquatic environment, fish can not only be used as an indicator of water purification, but also as an indicator of alien species invasion. At the same time, fish diversity indicators can also reflect their habitat status. In recent years, with the rapid development of my country's economy, ecological problems have become increasingly prominent, which provides convenient conditions for the invasion of alien species and brings hidden worries to the development and protection of local fish. How to quickly and effectively identify fish has become an urgent problem to be solved. However, because the traditional morphol...

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Problems solved by technology

However, because the traditional morphological taxonomy is not paid enough attention at present, the professionals of morphological taxonomy are gradually decreasing, which affects the work of fish resource protection and habitat research.

[0003] CN200510022323.1, a rapid identification method of fish molecular biology developed by Sichuan University in recent years, combines the conservation of nuclear DNA sequences encoding fish RNA and the interspecies differences of transcriptional spacers with the traditional Electrophoresis technology to identify fish, this method is time-consuming and costly, more importantly, this method cannot be used to screen and identify specific fish

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