High-flux shooting method used for flow cytometry imaging

Abstract

The invention belongs to the field of imaging technologies and provides a high-flux shooting method used for flow cytometry imaging. The high-flux shooting method used for flow cytometry imaging aims to solve the problems in the prior art that shooting efficiency is low, luminous flux is low and shooting missing exists. The method includes the following steps that cells move from top to bottom from a micro-pipeline; when the first cell at an even number enters a view field of a camera A, the camera A is driven by a transmission device to perform tracking shooting on the first even number; the camera A resets to an initial position through the transmission device after completing shooting, then shoots the cell at the next even number and transmits a collected image to a computer; when the first cell at an odd number enters a view field of a camera B, the camera B is driven by a transmission device to perform tracking shooting on the first odd number; the camera B resets to an initial position through the transmission device after completing shooting, then shoots the cell at the next odd number and transmits a collected image to the computer; the steps are repeated until all the cells are shot.

Description

technical field

[0001] The invention belongs to the technical field of imaging, and in particular relates to a high-throughput shooting method for imaging flow cytometers. Background technique

[0002] Cells are living bodies that make up various tissues and organs, and the health of cells directly affects human health. Microscopic detection of cells is an effective way to judge human health and discover potential tissue lesions, which requires clear and stable imaging of cells.

[0003] See attached figure 1 , the traditional method for cell detection is to let the cells pass through the field of view of the lens sequentially, and take pictures of the cells 1 sequentially. The device for realizing the above method includes a microchannel 2, a camera 4 and a computer 7. This method has only one lens, and the cells in the microchannel The flow velocity is V, and the cell 1 passes through the lens of the camera 4 sequentially. The integration time from entering the field of ...

Examples

Embodiment 1

[0031] See attached figure 2 , the device used to realize the high-throughput shooting method for imaging flow cytometer of the present invention includes micropipe 2, transmission device 3, camera A5, camera B6 and computer 7; said camera A5 and camera B6 respectively The tracking and shooting of the cells in the micro-channel 2 is realized by driving the transmission device 3 , and the movement of the transmission device 3 is controlled by a computer 7 .

[0032] A high-throughput shooting method for imaging flow cytometer of the present invention comprises the following steps:

[0033] Step 1: Cell 1 moves from top to bottom from microchannel 2, the flow rate of cell 1 is 2V, and the cells in microchannel 2 are sequentially numbered as n, n+1, n+2...n+m from bottom to top;

[0034] Step 2: When the n+1th cell enters the field of view of the camera A5, the camera A5 starts to work and takes pictures of the n+1th cell, and the camera A5 drives the n+1th cell by △V through t...

Embodiment 2

[0040] Cell 1 flows from top to bottom along the microchannel 2, we divide two adjacent cells 1 into a group, see attached figure 2, draw four groups of cells respectively, numbered as group 1 to group 4 from bottom to top according to the flow order of cell 1. And cell 1 is numbered from bottom to top, respectively: 1 group n, n+1, 2 groups n+2, n+3, 3 groups n+4, n+5 and 4 groups n+6, n+7 The cells in group 1 flow through camera A5 first, and then through camera B6. When cell n+1 in group 1 enters the field of view of camera A5, camera A5 starts to work, and camera A5 follows the cells under the action of transmission device 3. The flow of n+1 moves in the same direction to complete the exposure imaging of cell n+1 in the field of view of camera A for △t time, and then camera A5 quickly resets, waiting for the next group of cell 1 to be photographed. Then cell n of group 1 enters the field of view of camera B6, and cell n+3 of group 2 enters the field of view of camera A5....

technical field

[0001] The invention belongs to the technical field of imaging, and in particular relates to a high-throughput shooting method for imaging flow cytometers. Background technique

[0002] Cells are living bodies that make up various tissues and organs, and the health of cells directly affects human health. Microscopic detection of cells is an effective way to judge human health and discover potential tissue lesions, which requires clear and stable imaging of cells.

[0003] See attached figure 1 , the traditional method for cell detection is to let the cells pass through the field of view of the lens sequentially, and take pictures of the cells 1 sequentially. The device for realizing the above method includes a microchannel 2, a camera 4 and a computer 7. This method has only one lens, and the cells in the microchannel The flow velocity is V, and the cell 1 passes through the lens of the camera 4 sequentially. The integration time from entering the field of ...

Examples

Embodiment 1

[0031] See attached figure 2 , the device used to realize the high-throughput shooting method for imaging flow cytometer of the present invention includes micropipe 2, transmission device 3, camera A5, camera B6 and computer 7; said camera A5 and camera B6 respectively The tracking and shooting of the cells in the micro-channel 2 is realized by driving the transmission device 3 , and the movement of the transmission device 3 is controlled by a computer 7 .

[0032] A high-throughput shooting method for imaging flow cytometer of the present invention comprises the following steps:

[0033] Step 1: Cell 1 moves from top to bottom from microchannel 2, the flow rate of cell 1 is 2V, and the cells in microchannel 2 are sequentially numbered as n, n+1, n+2...n+m from bottom to top;

[0034] Step 2: When the n+1th cell enters the field of view of the camera A5, the camera A5 starts to work and takes pictures of the n+1th cell, and the camera A5 drives the n+1th cell by △V through t...

Embodiment 2

[0040] Cell 1 flows from top to bottom along the microchannel 2, we divide two adjacent cells 1 into a group, see attached figure 2, draw four groups of cells respectively, numbered as group 1 to group 4 from bottom to top according to the flow order of cell 1. And cell 1 is numbered from bottom to top, respectively: 1 group n, n+1, 2 groups n+2, n+3, 3 groups n+4, n+5 and 4 groups n+6, n+7 The cells in group 1 flow through camera A5 first, and then through camera B6. When cell n+1 in group 1 enters the field of view of camera A5, camera A5 starts to work, and camera A5 follows the cells under the action of transmission device 3. The flow of n+1 moves in the same direction to complete the exposure imaging of cell n+1 in the field of view of camera A for △t time, and then camera A5 quickly resets, waiting for the next group of cell 1 to be photographed. Then cell n of group 1 enters the field of view of camera B6, and cell n+3 of group 2 enters the field of view of camera A5....

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