Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of loquat leaf total sesquiterpene glycosides extract and its preparation method and application

A technology for total sesquiterpene glycosides and extracts, applied to total sesquiterpene glycosides extracts and their preparation, application fields for treating diabetes drugs or health care products, can solve problems such as lack of more extensive and in-depth research and the like

Active Publication Date: 2017-11-07
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As one of the important active components of loquat leaves, total sesquiterpene glycosides lack more extensive and in-depth research on its hypoglycemic activity and its mechanism of action

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of loquat leaf total sesquiterpene glycosides extract and its preparation method and application
  • A kind of loquat leaf total sesquiterpene glycosides extract and its preparation method and application
  • A kind of loquat leaf total sesquiterpene glycosides extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 1 kg of dried loquat leaves, pulverize them, and use 10 L of ethanol solution with a mass percentage concentration of 80% to extract twice at 70°C under reflux for 2 hours each time, combine the extracts, filter, concentrate under reduced pressure until there is precipitation, 3000 rpm Centrifuge for 15 minutes, and take the supernatant for later use;

[0016] Weigh Amberlite TM Add an appropriate amount of distilled water to XAD16 macroporous adsorption resin 8008, and place it overnight at room temperature to fully swell the resin. Pack the column by wet method, wash the column with 95% ethanol, and finally rinse the column with distilled water until the effluent has no ethanol smell. overnight;

[0017] Slowly flow the above supernatant through the above-mentioned treated and balanced macroporous resin column, first wash with deionized water, and then use ethanol gradients of different concentrations to elute, and collect eluents with an ethanol concentration b...

Embodiment 2

[0025] Embodiment 2: the total sesquiterpene glycoside content determination (HPLC method) of loquat leaf total sesquiterpene glycoside extract

[0026] Precisely weigh about 10 mg of compound I and compound II (self-made, its purity >95% by HPLC) as reference substance, dissolve in methanol, dilute and settle to 10mL volumetric flask, respectively as reference substance stock solution, and draw the standard curve. HPLC conditions: C18 chromatographic column (Dionex-Acclaim 120, 4.6×250mm, 5μm); column temperature 30°C; flow rate 1.0mL / min; injection volume 20μL; detection wavelength 210nm; mobile phase methanol (A)- Aqueous solution (B), isocratic elution (0-25 min, 70% A).

[0027] Accurately weigh 25.0 mg of the total sesquiterpene glycoside extract sample of loquat leaves, put it in a 50 mL volumetric flask, dissolve it in methanol, set the volume to the mark, filter to obtain the test solution, and measure it according to the above method. As shown in Figure 1, the cont...

Embodiment 3

[0028] Example 3: Determination of In Vitro Hypoglycemic Activity of Loquat Leaf Total Sesquiterpene Glycosides Extract - HepG2 Glucose Consumption Experiment

[0029] Cell lines and culture: human liver cancer cell line HepG2 was purchased from Shanghai Institute of Cells, Chinese Academy of Sciences, and inoculated in medium-sugar DMEM medium containing 10% calf serum (containing glucose 11.1mmol / L, supplemented with 100U / L of penicillin and streptomycin). ), placed in a 5% cell culture incubator at 37°C. HepG2 cells grew adherently, digested with 0.25% trypsin, and passaged once every 3 days at a ratio of 1:3. Cells in logarithmic growth phase were used for experiments. During the test, the cell suspension was inoculated in a 96-well culture plate at a density of 1.5×105, and cultured in an incubator to form a single layer of adherent cells.

[0030]Experimental method: 24 hours after inoculation, the cells had completely adhered to the wall and formed a monolayer cell lay...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a loquat leaf total sesquiterpene extract as well as a preparation method and application thereof. By taking dried leaves of eriobotrya japonica as a raw material, the preparation method comprises the following steps: carrying out reflux extraction on dried loquat leaves through ethanol at a certain concentration, filtering and concentrating at a reduced pressure; centrifugalizing concentrated liquor, adsorbing supernate by macroporous resin, gradient-eluting by water-ethanol, and collecting an ethanol eluting part in a certain concentration; and concentrating a collect fluid at a reduced pressure, carrying out polyamide column chromatography and water-methanol gradient elution to collect a methanol eluting part in certain concentration and recovering a solvent and drying to obtain the total sesquiterpene extract. The loquat leaf total sesquiterpene extract prepared by the method disclosed by the invention can be used for remarkably promoting glucose uptake of human hepatocellular carcinoma cells (HepG2) and indicating the hypoglycemic activity and can be researched and developed as a drug or health product for treating diabetes. The extract disclosed by the invention provides a scientific ground for application of loquat leaves for treating diabetes and provides a novel resource for developing safe and efficient novel anti-diabetes drug or health product.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a total sesquiterpene glycoside extract obtained from Chinese medicine loquat leaves, a preparation method thereof and an application in medicines for treating diabetes or health care products. Background technique [0002] Loquat leaf is the dried leaf of loquat (Eriobotrya japonica (Thunb.) Lindl.), a plant of the family Rosaceae. As a commonly used traditional Chinese medicine, it is recorded in the Chinese Pharmacopoeia for clinical use in traditional Chinese medicine. , used to treat symptoms such as cough due to lung heat, dyspnea due to gas inversion, vomiting due to stomach heat, dysphoria and thirst, etc. It is in line with the category of "diabetes" in the treatment of diabetes in traditional Chinese medicine. In 1988, Noreen et al. reported that the ethanol extract of loquat leaves had a significant but short-term hypoglycemic effect on normal r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A61P3/10A23L33/105A61K127/00A23L33/00
CPCA23V2002/00A61K36/73A61K2236/333A61K2236/39A61K2236/53A61K2236/55A23V2200/328A23V2250/21
Inventor 陈剑赵磊李维林任冰如敖先灿马丽吕寒刘艳
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com