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Pepsinogen II detection method and kit thereof

A pepsinogen and detection method technology, applied in the field of pepsinogen II dual-wavelength fluorescence immunochromatography detection method and its detection kit, can solve the problems of long detection time, complicated operation process, non-specific binding, etc. , to achieve the effect of reducing the impact of impurities

Inactive Publication Date: 2015-04-08
江苏宏泰格尔生物医学工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this patent can only eliminate the interference of some turbid substances, but does not eliminate some non-specific binding, and the detection result may be higher than the actual concentration
The Chinese patent publication number is CN102426236A. Although a method for detecting pepsinogen Ⅱ by combining chemiluminescence technology with immunomagnetic particles is announced, it is still an enzyme-linked immunoassay technology in essence. The operation process is complicated and the detection time is long. long

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  • Pepsinogen II detection method and kit thereof
  • Pepsinogen II detection method and kit thereof
  • Pepsinogen II detection method and kit thereof

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Experimental program
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Effect test

Embodiment 1

[0035] The dual-wavelength fluorescent immunochromatographic detection method of pepsinogen II of the present invention comprises the following steps:

[0036] 1) Preparation of immunochromatographic test strips: The pepsinogen II monoclonal antibody and chicken IgY were respectively coated on the detection line (T line) and control line (C line) of the chromatography test paper with a film spraying machine, After drying for 1-5 hours, cut with a strip cutter to obtain immunochromatographic test strips with a width of 3-5 mm;

[0037] It should be noted that: a) The traditional qualitative immunochromatography technology uses coated goat anti-mouse antibody as the control line. With the increase of pepsinogen II or some anti-mouse antibody blocking agents in the serum, the signal on the control line As it decreases, its signal value cannot be used for calculation, and the accuracy of the signal of the test line has no reference basis. The present invention adopts chicken IgY ...

Embodiment 2

[0048] Such as Figures 1 to 2 As shown, the dual-wavelength fluorescence immunochromatography detection kit of pepsinogen II of the present invention includes a kit body, a cryopreservation tube, and a diluent bottle, and the kit body includes a plastic liner 1 and a plastic liner fixed on the plastic liner. The sample pad 2, the immunochromatography test strip 3 and the absorbent paper 6, the immunochromatography test strip is made of nitrocellulose membrane material, the sample pad and the absorbent paper are respectively lapped on both sides of the immunochromatography test strip, and the immunochromatography test strip is A detection line 4 and a control line 5 are set on the chromatography test strip, and the detection line and the control line are respectively coated with pepsinogen II monoclonal antibody and chicken IgY; freeze-dried probes are stored in the cryopreservation tube, freeze-dried The probe is a freeze-dried probe prepared by reacting pepsinogen II monoclo...

specific Embodiment

[0052]The present invention also provides a specific embodiment of dual-wavelength fluorescence immunochromatographic detection of pepsinogen II, which includes the following steps in sequence:

[0053] Step 1: Preparation of lyophilized probes

[0054] 1) Mix two fluorescent latex particle solutions with emission wavelengths of 550nm and 700nm respectively according to the volume ratio of 1:1. After mixing evenly, take 500 μl of mixed fluorescent latex particle solution (containing carboxyl groups) and use pH6.0 MES buffer After washing and centrifuging for three times, the precipitate was diluted with pH6.0 MES buffer, mixed with 10 mg EDC, and then activated at room temperature for 30 minutes. After centrifugation, the precipitate was washed three times with pH6.0 MES buffer, and then the precipitate was washed with pH6. Dilute with 0MES buffer, add 125 μg of pepsinogen II monoclonal antibody, react at room temperature for 3 hours, add BSA to block, continue to react for 30...

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Abstract

The invention relates to a pepsinogen II detection method and a kit, and especially relates to a dual wavelength fluorescence immunity chromatography detection method of pepsinogen II and a detection kit thereof. The method comprises the following steps: 1)preparing an immunity chromatography test strip; 2)preparing a freeze-dried probe; 3)preparing a sample weak solution; and 4)examining the sample. The pepsinogen II detection method and the kit have the advantages of high sensitivity, high accuracy, simple operation and low cost.

Description

technical field [0001] The invention relates to a pepsinogen II detection method and a kit, in particular to a pepsinogen II dual-wavelength fluorescent immunochromatographic detection method and a detection kit. Background technique [0002] Pepsinogen (PG), the precursor of pepsin, is a single-chain polymorphism with a molecular weight of 42,000 Da. It is divided into two subgroups according to its biochemical properties and immunogenicity. Components 1-5 have the same immunogenicity and are called PGⅠ, which are mainly secreted by the principal cells and mucus neck cells of the gastric gland, and most of them enter the gastric cavity; components 6-7 are called PGⅡ, which are produced by the oxyntic glands of the gastric fundus The chief cells of the oxyntic glands, the mucous neck cells of the oxyntic glands, the mucous cells of the cardia glands and the pyloric glands of the gastric antrum, and the Brunner glands of the upper duodenum. Under normal circumstances, about ...

Claims

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Application Information

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IPC IPC(8): G01N33/573G01N33/533
CPCG01N33/6893G01N33/558G01N2333/90G01N2800/06
Inventor 张鹏张翼飞廖平璋张华
Owner 江苏宏泰格尔生物医学工程有限公司
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