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Optics and Microscopes

A technology of optical devices and microscopes, applied in the field of microscopes, can solve the problems of difficult realization of geometric shape and structural design, and achieve the effect of flexible mechanism

Active Publication Date: 2017-03-01
LEICA MICROSYSTEMS CMS GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This device requires a specially adapted sample holding mechanism, so it is often difficult to realize in terms of geometric shape and structural design.

Method used

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  • Optics and Microscopes
  • Optics and Microscopes
  • Optics and Microscopes

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Experimental program
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Effect test

Embodiment Construction

[0040] figure 1 It is a schematic diagram of the first embodiment of the optical device on the microscope of the present invention. The optical device has an illumination device for generating an illumination beam 1 extending on the illumination side. In addition, the optical device also has a beam splitting mechanism 2 and a mirror mechanism 5, the beam splitting mechanism 2 is used to divide the illumination light 1 into at least two sub-beams 3 and 4, and the mirror mechanism 5 is used to divide the two sub-beams 3 and 4 4 reflected into the illumination range 6 for planar illumination of the sample 7. In order to enable a flexible use of the structurally simple device, the optical device additionally has a detection optics 8 arranged on the side of the illumination field opposite the illumination side. In other words, all other optics for illuminating the sample 7 , such as the beam splitting means 2 and the mirror means 5 , are provided on the illuminating side in addit...

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Abstract

An optical device on a microscope with an illumination mechanism, a spectroscopic mechanism (2) and a reflector mechanism (5). The illumination mechanism is used to generate illumination light (1) extending on the illumination side. The spectroscopic mechanism ( 2) is used to divide the illumination light (1) into at least two sub-beams (3, 4), and the reflector mechanism (5) is used to reflect the sub-beams (3, 4) to the illumination range (6), for The sample (7) is plane illuminated, and for the sake of flexible application of a simple-structured mechanism, it is characterized by a detection optical mechanism (8) provided on the side of the illumination range (6) opposite to the illumination side. In addition, the present invention also proposes a microscope with corresponding optical devices.

Description

technical field [0001] The invention relates to an optical device on a microscope with an illumination device for generating an illumination beam extending on the illumination side, a beam splitter for splitting the illumination beam and a mirror device There are at least two sub-beams, and the mirror mechanism is used to reflect the sub-beams to an illumination range for planar illumination of the sample. Furthermore, the invention also relates to a microscope with corresponding optics. Background technique [0002] When observing living biological tissue with a light microscope, it is important to keep photoepitative damage to the sample as small as possible. For this purpose, a concept was developed for illuminating the detection plane of the microscope with a sheet light source. A microscopic imaging (Selective Plane Illumination Microscopy, SPIM) using selective plane illumination is realized here. In this case, the diffusion direction of the illumination light is pe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/00G02B21/36
CPCG02B21/0032G02B21/0076G02B21/367G02B21/06G02B21/18
Inventor 克里斯蒂安·舒曼
Owner LEICA MICROSYSTEMS CMS GMBH