Health-care food composition
A health food and composition technology, applied in food preparation, food science, food ingredients, etc., can solve the problems of insufficient immune function, unknown causes, and nutritional imbalance, so as to reduce the probability of cell disease, maintain human health, The effect of improving physiological functions
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Embodiment 1
[0024]
[0025] The composition according to the present invention includes a multivitamin component, a grape seed extract component, a green algae extract component, a pineapple enzyme component, a citrus flavonoid component, a curcumin extract component, and a lutein extract The source of each component, agarwood component, mineral component and blueberry extract component, are summarized in the following table-1.
[0026] Table-1 The source of each component in the composition
[0027] Component
Source
Comprehensive vitamin component
Kebu (Pfizer Pharmaceutical Co., Ltd.) a
Grape seed extract components
DSM Nutritional Products b
Chlorella extract components
DSM Nutritional Products b
Pineapple Enzyme Components
DSM Nutritional Products b
Citrus Flavonoids
DSM Nutritional Products b
[0028] Curcumin extract components
DSM Nutritional Products b
Lutein extract components
DSM Nutritional Products b
Agarwood components
DSM Nutritional Products b
M...
Embodiment 2
[0075]
[0076] The source and proportion of the composition (test substance) used in this example are the same As described in Table-1 and Table-2.
[0077] Cell source and culture environment:
[0078] 1. Cell lines: lung cancer cell LL2 and breast cancer cell BT-474, both purchased from the Food Industry Development Institute. Among them, the lung cancer cell is a mouse lung cancer cell (murine lewis lung carcinoma cell line), LL / 2 (LLC1) (BCRC60050), and the breast cancer cell is a human breast ductal carcinoma cell line, BT-474 (BCRC60359).
[0079] 2. Cultivation environment: containing 5% CO 2 (v / v) 37±2℃ incubator.
[0080] Grouping and dosage:
[0081] The experiment includes a control group and a different dose of the test group, each group of experiments are repeated three times:
[0082] Control group 1: Lung cancer cells LL2 control group: 90% DMEM and 10% FBS containing 4mM L-glutamine (Gibco, Lot: 482577).
[0083] Control group 2: Breast cancer cell BT-474 control group:...
Embodiment 3
[0112]
[0113] The source and proportion of the composition (test substance) used in this example are the same As described in Table-1 and Table-2.
[0114] Cell source and cell culture environment:
[0115] 1. Cell line: Mouse lung cancer cells (BCRC 60050, Lewis lung carcinoma, LLC), purchased from the Food Industry Development Institute.
[0116] 2. Cultivation environment: containing 5% CO 2 (v / v) 37±2℃ incubator.
[0117] 3. Cultivation method: Cultivation with DMEM containing 10% fetal bovine serum. After culturing a sufficient number of cells, wash with PBS to remove the serum, and use trypsin-EDTA to shed the cells, and centrifuge to collect the cells for use.
[0118] Experimental animals and breeding environment:
[0119] 1. Experimental animals: 12 6-week-old female C57BL / 6 mice with an average weight of 18±2 grams.
[0120] 2. Animal source: Lesco Biotechnology Co., Ltd.
[0121] 3. Feeding location and animal feeding management: Laboratory Animal Center of National Taiwan U...
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