Cultivation method and application for salidroside-containing plant
A technology of salidroside and cultivation method, which is applied in the field of biotransformation, can solve the problems of many by-products, high loss of raw materials and high cost, and achieves the effects of reduced usage, convenient operation and simple operation.
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Embodiment 1
[0038] Example 1: Motherwort Containing Salidroside
[0039] 1. Cultivation method: Spray 0.5% p-hydroxyphenylethanol aqueous solution to the leaves of the motherwort grown in hydroponics, and harvest the motherwort leaves on the third day.
[0040] A control group is set at the same time, the difference between the control group and the present embodiment is only that p-hydroxyphenethyl alcohol is not contained in the solvent sprayed to the blades.
[0041] 2, the detection of salidroside: the motherwort leaves obtained in this embodiment and the control group were cleaned and chopped, as two groups of samples to be tested; using high performance liquid chromatography, 5g of samples to be tested were respectively taken, and 25ml of 50% methanol was added , weighed, heated and refluxed for 60 minutes, allowed to cool, made up for lost weight, filtered, and used as two groups of test solution. Take another salidroside standard substance, add 50% methanol to make a solution con...
Embodiment 2
[0043] Example 2: Motherwort Containing Salidroside
[0044] 1. Cultivation method: Spray 10% p-hydroxyphenylethanol aqueous solution to the leaves of the motherwort grown in hydroponics, and harvest the motherwort leaves on the 15th day.
[0045] A control group is set at the same time, the difference between the control group and the present embodiment is only that p-hydroxyphenethyl alcohol is not contained in the solvent sprayed to the blades.
[0046] 2. Detection of salidroside: the detection method is the same as in Example 1.
[0047] It can be seen from the test results that the salidroside content (fresh weight) in the motherwort leaves obtained in this example is 0.028%, and no salidroside can be detected in the control group.
Embodiment 3
[0048] Example 3: Motherwort Containing Salidroside
[0049] 1. Cultivation method: Spray 5% p-hydroxyphenylethanol aqueous solution to the leaves of the motherwort grown in hydroponics, and harvest the motherwort leaves on the 7th day.
[0050] A control group is set at the same time, the difference between the control group and the present embodiment is only that p-hydroxyphenethyl alcohol is not contained in the solvent sprayed to the blades.
[0051] 2. Detection of salidroside: the detection method is the same as in Example 1.
[0052] It can be seen from the test results that the salidroside content (fresh weight) in the motherwort leaves obtained in this example is 0.032%, and no salidroside can be detected in the control group.
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