10 results about "UDP Glucose" patented technology

The application relates to the field of biological medicine, and discloses a glucosyltransferase mutant, a coding gene of the glucosyltransferase mutant, a recombination carrier, a recombination strain, an enzyme preparation, and application of the glucosyltransferase mutant. The glucosyltransferase mutant is a point mutation at the R position of the amino acid sequence shown in SEQ ID NO:1, and 4-8 histidines are added at the N end. A method for preparing gastrodin comprises the following steps: contacting the glucosyltransferase mutant with p-hydroxybenzyl alcohol and uridine diphosphate glucose. The glucosyltransferase mutant provided by the application has higher specificity for the substrate p-hydroxybenzyl alcohol, thereby obviously improving the conversion rate of the conversion of p-hydroxybenzyl alcohol into gastrodin, and having a good industrial application prospect.

The inventive technology described herein includes systems, methods, and compositions for the upregulation of Uridine diphosphate glucose (UDPG) recycling in a yeast cell, by expressing a heterologous nucleotide sequence, operably linked to a promoter, encoding a phosphoglucomutase enzyme which catalyzes the conversion from glucose-6-phosphate to glucose- 1 -phosphate, and a UDP-glucose pyrophosphorylase enzyme which catalyzes the formation of UDPG from glucose 1 -phosphate and UTP. The increased levels of UDPG substrate produced by the yeast cell may be used as a substrate for a co-expressed UDP -glycosyltransferases enzyme capable of generating cannabinoid glycoside compounds.

The application discloses application of uridine diphosphate glucose in promoting absorption of heavy metal cadmium by hyperaccumulating plants, and is characterized in that: a water solution of uridine diphosphate glucose is sprayed on leaf surfaces of hyperaccumulating plants in a growth stage, cadmium is enriched by using the growth and heavy metal absorption promoting effect of the uridine diphosphate glucose on the hyperaccumulating plants, and after the hyperaccumulating plants grow, the hyperaccumulating plants are cut or uprooted, and the application is obtained.The application sprays the water solution of the uridine diphosphate glucose on the leaf surfaces of the hyperaccumulating plants, uses the growth and heavy metal absorption promoting effect of the uridine diphosphate glucose on the plants to improve the cadmium accumulation amount of the hyperaccumulating plants, further improves the heavy metal enrichment efficiency, and effectively promotes efficient repair of heavy metal cadmium contaminated soil or water bodies by the hyperaccumulating plants.

The invention belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered bacterium for efficiently synthesizing betacyanin as well as a construction method and application of the genetically engineered bacterium. According to the invention, a gene editing technology is used for jointly enhancing the expression levels of phosphoglucomutase pgm and UDP-glucose pyrophosphorylase galU on a BL21 (DE3) genome, the supply level of uridine diphosphate glucose which is a precursor substance for synthesizing betacyanin can be effectively improved, and a genetically engineered bacterium is constructed by combining a double-plasmid multienzyme expression system and overexpressing hpaBC, dodA, CYP76AD1 and 5GT. The engineering bacterium realizes whole-cell catalytic synthesis of betacyanin by taking tyrosine and glucose as direct substrates for the first time through collaborative optimization of genome editing and a plasmid expression system, and the product yield and the process economy are remarkably improved.

The invention discloses a recombinant bacterium and an application of the recombinant bacterium in production of a tanshinone precursor 11, 20-dihydroxy ferruginol. The recombinant bacterium contains or expresses diterpenoid synthase KSL1 and TPS1, and galactokinase, difunctional UDP glucose 4-isomerase / aldose 1-isomerase and hexose-1-phosphate uridine transferase are knocked out or inhibited. According to the invention, de novo synthesis of 11, 20-dihydroxy ferruginol is realized through a plurality of strategies, and the yield of 11, 20-dihydroxy ferruginol is obviously improved. The recombinant strain provided by the invention can be used for efficiently producing 11, 20-dihydroxy ferruginol and other tanshinone precursors, and has an important application value.

A construction method of double immobilized recombinant yeast engineering bacteria, engineering bacteria, a catalyst and an application in synthesis of rebaudioside. In the present invention, yeast is used as the chassis cell, an ordered self-assembled multi-enzyme cascade surface display system is constructed, and carrier-free immobilization is coupled with uridine piphosphate glucose (UDPG) in-situ regeneration technology, resulting in a novel yeast whole cell catalyst with double immobilization of enzymes and bacteria. The catalyst uses cheap steviol glycoside (St) and rebaudioside A (Reb A) as substrates, and can synthesize rebaudioside (Reb D / M) in an efficient and economical one-pot method without additional addition of expensive UDPG. The synthesis process does not require cumbersome operations including cell disruption, enzyme separation, purification and immobilization, and avoids bottleneck problems in the related art including material transmembrane transport resistance, mass transfer resistance and product hydrolysis by intracellular enzyme. The catalyst has reusability, and can realize continuous and high-intensity rebaudioside biosynthesis through strain rejuvenation, which provides strong technical support for its industrial application.

The present invention discloses a method for enzymatically synthesizing nucleotide sugars, which belongs to the field of biotechnology. The present invention uses sucrose as a substrate, and generates uridine diphosphate glucose and fructose by sucrose synthase catalysis, fructose is catalyzed by mannose isomerase or lyxose isomerase to generate mannose, mannose is catalyzed by N-acetyhexose aminophosphokinase to generate mannose-1-phosphate, and mannose-1-phosphate is catalyzed by GDP-Man pyrophosphorylase to obtain guanosine diphosphate mannose. The present invention cascades modules for synthesizing UDP-Glc and synthesizing GDP-Man, uses cheap sucrose as a raw material, and simultaneously obtains UDP-Glc and GDP-Man in the shortest reaction route, thereby reducing the cost of synthesizing high-value-added oligosaccharides and glycosides.

This invention discloses a method for extracting mogroside V, relating to the field of biotechnology. The method involves culturing, fermenting, centrifuging, and homogenizing *E. coli* containing glycosyltransferases UGT-MS1 and UGT-MS2 to obtain an enzyme supernatant. Then, mogroside IIE, uridine diphosphate glucose, the enzyme supernatant, and salt are added to a phosphate buffer solution for enzymatic catalysis to obtain a conversion solution. Finally, the solution is ultrafiltered, loaded onto a column, eluted, and then passed through an alkaline anion exchange resin. The effluent is concentrated and dried to obtain mogroside V. The product has a high yield and is environmentally friendly.

The invention discloses a drug target protein Ugd and application thereof in agriculture, and relates to the technical field of agricultural biology. The UDP glucose 6-dehydrogenase protein (Ugd) is identified from sclerotium rolfsii and fusarium graminearum through target stability analysis of a drug affinity reaction by taking a plant source natural product butenyl phthalide as a probe; according to the application disclosed by the invention, the Ugd protein is identified as a potential action target of butenyl phthalide for the first time, functional domains of the Ugd protein in sclerotium rolfsii and fusarium graminearum are defined, amino acids at the 371-466th sites of the fusarium graminearum Ugd protein form an important functional domain, amino acids at the 336-452 sites of the sclerotium rolfsii Ugd protein form a key functional domain, and the Ugd protein is identified as the potential action target of butenyl phthalide. The functional domains can be used as specific receptor sites for designing targeted drugs, and the drugs specifically bound with the Ugd protein can be accurately designed according to molecular structure characteristics of the functional domains and the structural domains through technologies such as molecular docking and the like.

The utility model discloses an extraction device of mogroside V, and relates to the technical field of biology. An inlet of a reaction tank is respectively communicated with a fermentation liquid tank, a mogroside IIE tank, a uridine diphosphate glucose tank and a salt tank through pipelines, and an outlet of the reaction tank is communicated with a conversion liquid tank through a pipeline; an outlet of the conversion liquid tank is communicated with a macroporous resin column through a pipeline, an inlet of the macroporous resin column is communicated with an eluent tank through a pipeline, an outlet of the macroporous resin column is communicated with an eluent tank, and an outlet of the eluent tank is communicated with an anion exchange resin column through a pipeline. An outlet of the anion exchange resin column is communicated with a concentration tank, an outlet of the concentration tank is communicated with a dryer, and an outlet of the dryer is communicated with a mogroside V tank. The yield and the purity of the product are improved, and the production steps and the production period are shortened.