A kind of sea cauliflower seedling tissue culture rapid propagation method
A technology for sea cauliflower and seedlings, applied in the field of plant biology, can solve problems such as difficulty in disinfection and sterilization, and achieve the effects of fast reproduction, high application value and large yield
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[0047] Preparation of medium:
[0048] Preparation of organic additives: take the required amount of acid hydrolyzed casein with an electronic balance and directly dissolve it in an appropriate amount of deionized water to form an aqueous solution for later use; coconut juice is fresh coconut fruit purchased from the market, and the juice in the fruit is taken and mixed with double Filter through a layer of gauze and set aside.
[0049] The composition and content of the improved MS are: (1) NH 4 NO 3 1650 mg / L, (2) KNO 3 1900 mg / L, (3) CaCl 2 ﹒ 2H 2 O 440 mg / L, (4) MgSO 4 ﹒ 7H 2 O 370 mg / L, (5) KH 2 PO 4 170 mg / L, (6)Na 2 -EDTA 37.3 mg / L, (7) FeSO 4 ﹒ 7H 2 O 27.8 mg / L, (8)MnSO 4 ﹒ 4H 2 O 22.3 mg / L, (9)ZnSO 4 ﹒ 7H 2 O 8.6 mg / L, (10)H 3 BO 3 6.2 mg / L, (11) KI 0.83 mg / L, (12) Na 2 MoO 4 ﹒ 2H 2 O 0.25 mg / L, (13)CuSO 4 ﹒ 5H 2 O 0.025 mg / L, (14) CoCl 2 ﹒ 6H 2 O 0.025 mg / L, (15) thiamine hydrochloride VB 1 0.3 mg / L, (16) Niacin 0.5 mg / L, (17) ...
Embodiment 1
[0052] Sea cauliflower seedling tissue culture rapid propagation method comprises the following specific steps:
[0053] (1) Fruit treatment and disinfection
[0054] Collect the complete sea cauliflower fruit with fully expanded fruit, dark blue or light yellow skin, and no pests and diseases from the distribution area of Guilin Yongfu sea cauliflower, and bring it back to the laboratory with a fresh-keeping bag to keep it moist. For the fruit, see figure 1 .
[0055] Use a razor blade to gently remove the attachments on the surface of the peel, then use gauze to take a small amount of detergent to scrub clean, and then soak in 150 mg / L thiophanate-methyl solution for 0.3 h, stir constantly, and rinse with tap water.
[0056] Soak in 70% (v / v) alcohol for 2 minutes on the ultra-clean workbench, then soak in 0.2% (w / v) mercury chloride for 15 minutes with constant stirring, and rinse with sterile water 5 to 6 times. The success rate of disinfection was 97.5%.
[0057] (2...
Embodiment 2
[0062] Sea cauliflower seedling tissue culture rapid propagation method comprises the following specific steps:
[0063] (1) Fruit treatment and disinfection
[0064] Collect and clean sea cauliflower fruit in the same way as in Example 1, then soak them in 100 mg / L thiophanate-methyl solution for 1 h, and rinse them with tap water; soak them in 75% (v / v) alcohol for 1 hour on the ultra-clean workbench. min, then soaked in 0.1% (w / v) mercuric chloride for 20 min, and rinsed with sterile water 5-6 times. The disinfection success rate is 95%.
[0065] (2) Cut the sterilized sterile fruit longitudinally with a scalpel, select seeds with black or brown seed coats and connect them to the germination medium for cultivation. The medium is: MS, 6-BA 1.0 mg / L , NAA 0.1 mg / L, agar 5.5 g / L, sucrose 30 g / L, pH 5.8. Add 4-6 cm of sterile water to the surface of the medium, and the culture condition is dark culture at a temperature of 28±2°C. The germination rate in 15 days was 98.6%. ...
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