A kind of sea cauliflower seedling tissue culture rapid propagation method

A technology for sea cauliflower and seedlings, applied in the field of plant biology, can solve problems such as difficulty in disinfection and sterilization, and achieve the effects of fast reproduction, high application value and large yield

Inactive Publication Date: 2016-08-31
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of propagation method that utilizes plant tissue culture technology to obtain a large amount of sea cauliflower seedlings fast, has the characteristics such as fast propagation speed, good seedling quality, operability, and overcomes the external planting method in aquatic plant tissue culture. Difficulty in body disinfection and sterilization

Method used

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  • A kind of sea cauliflower seedling tissue culture rapid propagation method
  • A kind of sea cauliflower seedling tissue culture rapid propagation method
  • A kind of sea cauliflower seedling tissue culture rapid propagation method

Examples

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Effect test

preparation example Construction

[0047] Preparation of medium:

[0048] Preparation of organic additives: take the required amount of acid hydrolyzed casein with an electronic balance and directly dissolve it in an appropriate amount of deionized water to form an aqueous solution for later use; coconut juice is fresh coconut fruit purchased from the market, and the juice in the fruit is taken and mixed with double Filter through a layer of gauze and set aside.

[0049] The composition and content of the improved MS are: (1) NH 4 NO 3 1650 mg / L, (2) KNO 3 1900 mg / L, (3) CaCl 2 ﹒ 2H 2 O 440 mg / L, (4) MgSO 4 ﹒ 7H 2 O 370 mg / L, (5) KH 2 PO 4 170 mg / L, (6)Na 2 -EDTA 37.3 mg / L, (7) FeSO 4 ﹒ 7H 2 O 27.8 mg / L, (8)MnSO 4 ﹒ 4H 2 O 22.3 mg / L, (9)ZnSO 4 ﹒ 7H 2 O 8.6 mg / L, (10)H 3 BO 3 6.2 mg / L, (11) KI 0.83 mg / L, (12) Na 2 MoO 4 ﹒ 2H 2 O 0.25 mg / L, (13)CuSO 4 ﹒ 5H 2 O 0.025 mg / L, (14) CoCl 2 ﹒ 6H 2 O 0.025 mg / L, (15) thiamine hydrochloride VB 1 0.3 mg / L, (16) Niacin 0.5 mg / L, (17) ...

Embodiment 1

[0052] Sea cauliflower seedling tissue culture rapid propagation method comprises the following specific steps:

[0053] (1) Fruit treatment and disinfection

[0054] Collect the complete sea cauliflower fruit with fully expanded fruit, dark blue or light yellow skin, and no pests and diseases from the distribution area of ​​Guilin Yongfu sea cauliflower, and bring it back to the laboratory with a fresh-keeping bag to keep it moist. For the fruit, see figure 1 .

[0055] Use a razor blade to gently remove the attachments on the surface of the peel, then use gauze to take a small amount of detergent to scrub clean, and then soak in 150 mg / L thiophanate-methyl solution for 0.3 h, stir constantly, and rinse with tap water.

[0056] Soak in 70% (v / v) alcohol for 2 minutes on the ultra-clean workbench, then soak in 0.2% (w / v) mercury chloride for 15 minutes with constant stirring, and rinse with sterile water 5 to 6 times. The success rate of disinfection was 97.5%.

[0057] (2...

Embodiment 2

[0062] Sea cauliflower seedling tissue culture rapid propagation method comprises the following specific steps:

[0063] (1) Fruit treatment and disinfection

[0064] Collect and clean sea cauliflower fruit in the same way as in Example 1, then soak them in 100 mg / L thiophanate-methyl solution for 1 h, and rinse them with tap water; soak them in 75% (v / v) alcohol for 1 hour on the ultra-clean workbench. min, then soaked in 0.1% (w / v) mercuric chloride for 20 min, and rinsed with sterile water 5-6 times. The disinfection success rate is 95%.

[0065] (2) Cut the sterilized sterile fruit longitudinally with a scalpel, select seeds with black or brown seed coats and connect them to the germination medium for cultivation. The medium is: MS, 6-BA 1.0 mg / L , NAA 0.1 mg / L, agar 5.5 g / L, sucrose 30 g / L, pH 5.8. Add 4-6 cm of sterile water to the surface of the medium, and the culture condition is dark culture at a temperature of 28±2°C. The germination rate in 15 days was 98.6%. ...

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Abstract

The invention discloses a method for rapid propagation of sea cauliflower seedlings through tissue culture, inducing the germination of sea cauliflower seeds to form sterile sprouts, and then using the sterile sprouts as materials to inoculate on the screened subculture propagation medium, and subculture In 25 days, the reproduction coefficient is more than 3 times, and the sprouts are strong and the leaves are dark green. Afterwards, the sprouts with a plant height of 4 cm are transferred to the rooting medium. The rooting rate is as high as 100% in 30 days. Seedlings are transplanted in waters with good water quality and flowing waters, the survival rate is over 85%, and a large number of proliferations can be obtained in a relatively short period of time. To a certain extent, industrialized tissue culture seedling production can be realized. It also has the characteristics of fast propagation speed, large output, excellent seedling quality, short time required, strong practical operation, high application value, etc., which has great significance and good application prospects for the protection and sustainable utilization of sea cauliflower species.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a rapid propagation method for tissue culture of sea cauliflower seedlings containing high protein. Background technique [0002] sea ​​cauliflower Ottelia acuminata (Gagnep.) Dandy is a large perennial submerged plant, endemic to my country, and a national second-class protected plant. Sea cauliflower can absorb a large amount of nutrients in water, reduce the content of nitrogen and phosphorus, and can also secrete some allelochemical substances to inhibit the growth of algae. Therefore, it plays an important role in solving ecological problems such as eutrophication and algal blooms in water bodies, and is known as a purifier. The "touchstone" of water quality. Sea cauliflower is rich in protein, calcium, phosphorus and other nutrients. It is a very popular green vegetable, and it is also the main source of feed for people in production areas to develop aquacul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 唐凤鸾黄宁珍何金祥赵健付传明冼康华仇硕黄惠锦
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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