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Method for extraction of polysaccharide from yeast cell wall

A yeast cell wall and polysaccharide technology, applied in the field of extracting polysaccharides, can solve the problems of low yield, large amount of acid and alkali, serious environmental pollution, etc., and achieve the effects of high yield, simple method and high purity

Inactive Publication Date: 2015-05-13
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology makes it easier than traditional methods that take longer or require more expensive equipment. It allows us to make better ways to isolate specific types of carbohydrates from other substances like proteins without damaging them too much.

Problems solved by technology

The technical problem addressed by this patented technology relates to how to prepare pure yeast cells with specific properties for use in various applications like animal feeds or medical treatments that require them due to their unique structure and composition.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Extraction of polysaccharides from fresh Saccharomyces cerevisiae:

[0023] (1) Insert Saccharomyces cerevisiae stored in a glycerol tube at -80°C into 10mL YPD culture solution (1% yeast extract, 2% peptone, 2% glucose), and culture with shaking at 30°C for 20 hours;

[0024] (2) Use the culture solution in (1) as a seed, insert it into a conical flask of 500mL YPD liquid medium, shake and cultivate for 20h, centrifuge at 8000rpm for 10min, and wash the thalline with deionized water;

[0025] (3) Take 5 g of the thallus in (2) and suspend in the extract of 100 mL of lithium acetate-SDS preheated in advance, wherein the concentration of lithium acetate is 50 mmol / L, and the mass fraction of SDS is 1%;

[0026] (4) Incubate at 70°C for 10 minutes, shake the Erlenmeyer flask every 2.5 minutes to make the extract fully contact with the bacteria, and obtain a crude extract of polysaccharides.

[0027] (5) Transfer the extract in the Erlenmeyer flask to a centrifuge tube, a...

Embodiment 2

[0031] Extraction of polysaccharides from fuel ethanol fermentation waste yeast:

[0032] (1) Take 10 g of fuel ethanol fermentation waste yeast and suspend it in the preheated 200 mL lithium acetate-CTAB extract, wherein the concentration of lithium acetate is 100 mmol / L, and the mass fraction of CTAB is 1%;

[0033] (2) Incubate at 50°C for 20 minutes, shake the Erlenmeyer flask every 2.5 minutes to make the extract fully contact with the bacteria, and obtain a crude extract of polysaccharides.

[0034] (3) Transfer the extract in the Erlenmeyer flask to a centrifuge tube, and centrifuge at 5000rpm for 10min to remove cell fragments.

[0035] (4) After collecting the supernatant, use 1 volume of 95% ethanol to precipitate the polysaccharide at 4-25° C., centrifuge at 10,000 rpm for 10 min, collect the precipitate (polysaccharide), and freeze-dry to obtain zymosan.

[0036] Through the above method, the extraction rate of polysaccharides in waste yeast for fuel ethanol ferme...

Embodiment 3

[0038] Extraction of polysaccharides from Rhodotorula viscosus:

[0039] (1) Using Rhodotorula viscosus preserved in a glycerol tube as a seed, the process of cultivating and collecting the thalline is the same as in Example 1;

[0040] (2) 5 g of Rhodotorula viscosus were suspended in 25 mL of lithium acetate-CTAB-SDS extract preheated in advance, wherein the concentration of lithium acetate was 300 mmol / L, and the mass fraction of CTAB and SDS was 1%;

[0041] (3) Incubate at 90° C. for 5 minutes, during which time the flask is shaken, so that the extract and the bacteria are in full contact, and the crude extract of polysaccharides is obtained.

[0042] (4) After extraction, the polysaccharide solution can be directly wound with a glass rod, scraped off, and freeze-dried to obtain zymosan powder.

[0043] Through the above method, the polysaccharide extraction rate of Rhodotorula viscosus can reach 17.3%, and the polysaccharide content measured by the phenol sulfuric acid ...

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Abstract

The invention provides a method for extraction of polysaccharide from yeast cell wall, cultured fresh yeast or industrial waste yeast as a raw material is suspended in an extraction liquid containing a lithium salt and a surface active agent for extraction of polysaccharide in a water bath; the method for extraction of polysaccharide has the advantages of short zymosan extraction time and high yield, can be used for scale extraction of yeast cell wall polysaccharides, and has great significance for yeast high value utilization.

Description

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Claims

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Application Information

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Owner JIANGSU UNIV
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